1.
Microsc Microanal
; 29(Supplement_1): 2068-2069, 2023 Jul 22.
Artigo
em Inglês
| MEDLINE
| ID: mdl-37612964
2.
Nat Methods
; 5(4): 311-3, 2008 Apr.
Artigo
em Inglês
| MEDLINE
| ID: mdl-18345005
RESUMO
The difficulty in localizing specific cellular proteins by immuno-electron microscopy techniques limits applications of electron microscopy to cell biology. We found that in vivo immunogold labeling improves epitope accessibility, ultrastructural preservation and three-dimensional visualization, and allows correlated light and electron microscopy. We detected large-scale chromatin folding motifs within intact interphase nuclei of CHO cells and visualized the ultrastructure of DNA replication 'factories' labeled with GFP-proliferating cell nuclear antigen (PCNA).