RESUMO
Mangabeys, macaques, and baboons persistently infected with human immunodeficiency virus (HIV)-2 NIH-DZ demonstrated no signs of immunodeficiency disease after 6-11 months following seroconversion. Thus Old World monkeys provide an animal model to investigate the effects of passive immunization (anti-HIV-2 antibodies) on HIV infection in primates.
Assuntos
Síndrome da Imunodeficiência Adquirida/microbiologia , Cercopithecidae , Modelos Animais de Doenças , HIV-2/isolamento & purificação , Síndrome da Imunodeficiência Adquirida/prevenção & controle , Animais , Western Blotting , Ensaio de Imunoadsorção Enzimática , Anticorpos Anti-HIV/análise , Antígenos HIV/análise , HIV-2/imunologia , HIV-2/ultraestrutura , Imunização Passiva , Macaca mulatta , PapioRESUMO
Six monkeys of three different species (mangabey, macaque and baboon) were infected with human immunodeficiency type 2 (HIV-2) NIH-DZ using intraperitoneal or intravenous injections of cell-free HIV-2 or autologous HIV-2-infected cells with no prior immunostimulation. Viral expression was demonstrated by reverse transcriptase activity in cells after coculture with human peripheral blood lymphocytes or by electron microscopy. Serum was analyzed by western blot, enzyme-linked immunosorbent assay (detection of antigen and antibody), and neutralization assay carried out using immunofluorescence techniques. The 6 inoculated animals seroconverted during the 1st month after inoculation and remained persistently infected after 6-11 months. We also observed proviral DNA by genomic analysis in the six tested samples. No sign of immunodeficiency disease has been observed so far. The data suggest that HIV-2 infection of nonhuman primates provides an acceptable animal model to investigate vaccination or specific immunotherapeutic procedures.
