Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 2 de 2
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Liver Transpl ; 25(9): 1375-1386, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-31121085

RESUMO

Organ transplantation is the treatment of choice against terminal and irreversible organ failure. Optimal preservation of the graft is crucial to counteract cold ischemia effects. As we developed an N,N-bis-2-hydroxyethyl-2-aminoethanesulfonic acid-gluconate-polyethylene glycol (BGP)-based solution (hypothermic machine perfusion [HMP]), we aimed to analyze the use of this solution on static cold storage (SCS) of rat livers for transplantation as compared with the histidine tryptophan ketoglutarate (HTK) preservation solution. Livers procured from adult male Sprague Dawley rats were preserved with BGP-HMP or HTK solutions. Liver total water content and metabolites were measured during the SCS at 0°C for 24 hours. The function and viability of the preserved rat livers were first assessed ex vivo after rewarming (90 minutes at 37°C) and in vivo using the experimental model of reduced-size heterotopic liver transplantation. After SCS, the water and glycogen content in both groups remained unchanged as well as the tissue glutathione concentration. In the ex vivo studies, livers preserved with the BGP-HMP solution were hemodynamically more efficient and the O2 consumption rate was higher than in livers from the HTK group. Bile production and glycogen content after 90 minutes of normothermic reperfusion was diminished in both groups compared with the control group. Cellular integrity of the BGP-HMP group was better, and the histological damage was reversible. In the in vivo model, HTK-preserved livers showed a greater degree of histological injury and higher apoptosis compared with the BGP-HMP group. In conclusion, our results suggest a better role of the BGP-HMP solution compared with HTK in preventing ischemia/reperfusion injury in the rat liver model.


Assuntos
Transplante de Fígado/métodos , Soluções para Preservação de Órgãos/administração & dosagem , Preservação de Órgãos/métodos , Perfusão/métodos , Traumatismo por Reperfusão/prevenção & controle , Ácidos Alcanossulfônicos/química , Aloenxertos/irrigação sanguínea , Aloenxertos/patologia , Animais , Isquemia Fria/efeitos adversos , Modelos Animais de Doenças , Gluconatos/administração & dosagem , Gluconatos/química , Glucose/administração & dosagem , Humanos , Fígado/irrigação sanguínea , Fígado/patologia , Transplante de Fígado/efeitos adversos , Masculino , Manitol/administração & dosagem , Soluções para Preservação de Órgãos/química , Polietilenoglicóis/administração & dosagem , Polietilenoglicóis/química , Cloreto de Potássio/administração & dosagem , Procaína/administração & dosagem , Ratos , Traumatismo por Reperfusão/diagnóstico , Traumatismo por Reperfusão/etiologia , Traumatismo por Reperfusão/patologia , Fatores de Tempo
2.
Cryobiology ; 72(3): 191-7, 2016 06.
Artigo em Inglês | MEDLINE | ID: mdl-27164058

RESUMO

Although primary neuronal cells are routinely used for neuroscience research, with potential clinical applications such as neuronal transplantation and tissue engineering, a gold standard protocol for preservation has not been yet developed. In the present work, a slow cooling methodology without ice seeding was studied and optimized for cryopreservation of rat cerebellar granular cells. Parameters such as cooling rate, plunge temperature and cryoprotective agent concentration were assessed using a custom built device based on Pye's freezer idea. Cryopreservation outcome was evaluated by post thawing cell viability/viable cell yield and in culture viability over a period of 14 days. The best outcome was achieved when 10% of Me2SO as cryoprotective agent, a cooling rate of 3.1 ± 0.2 °C/min and a plunge temperature of -48.2 ± 1.5 °C were applied. The granular cells cryopreserved under these conditions exhibited a cell viability of 82.7 ± 2.7% and a viable cell yield of 28.6 ± 2.2%. Moreover, cell viability in culture remained above 50%, very similar to not cryopreserved cells (control). Our results also suggest that post-thaw viability (based on membrane integrity assays) not necessarily reflects the quality of the cryopreservation procedure and proper functionality tests must be carried out in order to optimize both post thaw viability/cell yield and in culture performance.


Assuntos
Criopreservação/métodos , Neurônios , Animais , Sobrevivência Celular/efeitos dos fármacos , Cerebelo/citologia , Crioprotetores/farmacologia , Feminino , Masculino , Ratos Sprague-Dawley , Temperatura
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...