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Maralfalfa (Cenchrus purpureus (Schumach.) Morrone) is a productive fodder crop in tropical regions that has been evaluated for forage nutritional value in a Mediterranean climate. To assess the nutritional value, parameters including dry matter content (DM), ash, ether extract (EE), protein (CP), fiber contents (NDF and ADF), and the amino acids profile were determined at eight harvest times (HTs) in a non-fertilized and non-irrigated crop based in Silla (Valencia, Spain). The results showed significant differences in most of the parameters studied. While CP and ash significantly decreased over the eight HTs, NDF and ADF increased. In contrast, EE and the ratio of essential amino acids/total amino acids remained constant. Values of CP remained higher than 15% during the first two HTs (16 and 28 days). According to the analyses performed, the optimum HT can be stated at 28 days as it combines high levels of CP (including an optimal combination of essential amino acids) with low levels of fibers (NDF = 57.13%; ADF = 34.76%) and a considerable amount of dry matter (15.40%). Among the essential amino acids (EA) determined, lysine and histidine showed similar values (Lys ≈ 6%, His ≈ 1.70%) when comparing the composition of these EA to other forage species and cultivars studied, whereas methionine showed lower values. This work establishes the basis for the appropriate HT of maralfalfa according to the nutritional parameters measured. Further studies could be aimed to optimize the nutritional and phytogenic properties of maralfalfa to improve its value as a fodder crop, and to finally introduce it for sustainable livestock production in Mediterranean countries.
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Tetraclinis articulata (Vahl) Masters is an endangered tree growing in coastal and arid environments that is widely exploited by the timber and resin industry, among other applications. In this context, the use of in vitro techniques is highly encouraged for its propagation. We present a protocol for micropropagation using twigs from adult trees as a source of explants. The Schenk and Hildebrandt basal medium (SH) supplemented with 30 g L-1 sucrose, 6.5 g L-1 plant agar, 4.0 mg L-1 6-benzyladenine (BA), and 0.05 mg L-1 1-naphthaleneacetic acid (NAA) provided the optimum multiplication rate (90.48 ± 9.52 explants with basal shoots and 2.58 ± 0.29 basal shoots per explant). Application of activated charcoal (AC) or ½ Knop solution in a liquid overlay produced significantly longer shoots. Supplementation of solid media with indole-3-butyric acid (IBA) or NAA gave low rooting percentages (<17%). Addition of 0.9 g L-1 AC improved rooting (40%) but rooting performance was optimal (66.7%) after a pulse treatment consisting of 4 h immersion in liquid SH medium without growth regulators, followed by 8 weeks of cultivation. Rooted microplants were successfully acclimatized (93.33%) in a peat moss and vermiculite mixture (1:1 v/v ratio). The genetic stability of the in vitro regenerated plantlets was confirmed using the randomly amplified polymorphic DNA (RAPD) technique. Explant survival and growth remained higher than 90% after 28 weeks of cold storage at both 4 °C and 10 °C. The protocol presented here allows for largescale T. articulata production and could be applied for both ex situ conservation strategies and industrial purposes.
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Opuntia spp. are cacti with high ecological, economic and conservation interest in semiarid environments, particularly in Mexico. We conducted a systematic search of the existing peer-reviewed literature about the state of knowledge of pollination ecology on these plants. We documented the most studied Opuntia species worldwide with an emphasis on Mexico. We found that only 15% of Opuntia species described have been investigated so far, and studies were mainly focused on comprehension of the biology of a single species. Despite the economic and cultural importance of Opuntia, there is a significant lack of knowledge about the flower-visiting insects and their taxonomic identity. We provide a checklist of the insect species associated with Opuntia spp. Through a circular network, we visualize the complex Opuntia flower-visiting insect relationship, and we detected a set of key species constituting the generalist core of the networks constructed. Since pollination is crucial for crop production, a better understanding of ecological interactions would inform management measures to strengthen biodiversity and agriculture sustainability as well as productivity in arid and marginal lands. Further research on pollination ecology is needed to improve the conservation status of the insects associated with Opuntia species.
RESUMO
ETHNOPHARMACOLOGICAL RELEVANCE: Species of the genera Hieracium and Pilosella have been used in folk medicine for centuries in many parts of the world. The most wiedly used species is P. officinarum Vaill., included in the British and French Pharmacopoeias and sold as part of different commercial products. AIM OF THE STUDY: This review critically appraises the state-of-art of ethnopharmacology, specialised metabolites, bioactivities, and toxicity of members of Hieracium and Pilosella. Thus, gaps in scientific knowledge can be identified, also focusing on the development of products with pharmacological applications. MATERIALS AND METHODS: Literature data of Hieracium and Pilosella species were mainly retrieved using different electronic databases such as Web of Science, Google Scholar, SciFinder, and PubMed. Other electronic resources included worldwide databases on ethnobotany, ethnopharmacology, and phytochemistry as well as government reports. Additionally, ancient texts and local information such as PhD and MSc theses, and books were consulted. RESULTS: A comprehensive analysis of the above mentioned sources revealed that only 34 out of the about 850 described species within the genera Hieracium and Pilosella have been reported in the context of traditional medicinal and ethnobotanical knowledge. The most often mentioned species is P. officinarum which has been widely used due to its diuretic effects. Other popular uses of Hieracium and Pilosella species include the treatment of skin, gastric, and intestinal diseases as well as respiratory and vascular ailments. Moreover, taxa of the two genera have been used as antiobiotics, antiseptics, antidiabetics, tonics, antiepileptics, antiphlogistics, emetics, wound healing drugs, astringents, haemostatics, and detoxificants. Finally, uses as a wild vegetable, fodder, plant for hunting and for charming rituals have also been mentioned. Phytochemical research revealed a richness in phenolic compounds and flavonoids. Moreover, coumarins, sesquiterpene lactones, terpenoids, and phytosterols were found in Hieracium and Pilosella. Experimental research conducted to support traditional uses mainly include in vitro tests, while assays based on in vivo models (including humans) are rather limited. Also, the vast majority of the studies did not identify the compounds responsible for the detected bioactivities. These established bioactivities include antidiabetic, anti-inflammatory, antibacterial, antimycotic, antiviral, cytotoxic and antiproliferative, diuretic, gastroprotective, antiepileptic, hypotensive, anti-obesity, arthropodicidal, and skin rejuvenating activities. Finally, limited toxicity studies have been conducted on members of Hieracium and Pilosella. CONCLUSION: Taxa belonging to Hieracium and Pilosella have been confirmed to exert diuretic, anti-inflammatory, and antimicrobial effects, which is in line with their long traditional use. Moreover, the above mentioned fields of application hint to the most promising routes for the development of new marketable products. Nonetheless, additional data from an in-depth research on bio-active specialised metabolites such as sesquiterpenoids, sesquiterpene lactones, and coumarines, their bioactivities and toxicity, and their biosynthesis are still warranted.
