[Changes of microcirculation in healthy volunteers and patients with septic shock in Xining].

OBJECTIVE: The purpose of this study is to investigate the characteristic of microcirculation in healthy volunteers and patients with septic shock in both Xining (2 260 m) and Nanjing (10 m). METHODS: A total of 62 cases, 33 healthy volunteers, 22 cases in Xining, (2 260 m above sea level) and 11 cases in Nanjing (10 m above sea level); and 29 septic shock, 13 cases in Xining and 16 cases in Nanjing were collected. The total vessel density (TVD), perfused vessel density (PVD), proportion of perfused vessel (PPV) and microcirculation flow index (MFI) of both healthy volunteers and septic shock had been investigated by using sidestream dark field (SDF). Analyzed and managed the image data by using AVA3.0 software. RESULTS: In the healthy volunteers in Xining area (22 cases),the volume of TVD (15.59 ±2.58 mm/mm2), PVD (15.58 ±2.58 mm/mm2) and PPV(96.60% ±4.63%) were significant higher than the volume of TVD (10.0 ±2.10 mm/mm2), PVD (10.81 ±2.38 mm/mm2) and PPV (84.24% ±8.00%) of the volunteers (11 cases) in Nanjing (11 cases). But the MFI (2.17 ±0.31) of the healthy volunteers in the Xining was significant lower (P<0.05) than the MFI (3.21 ±0.34) in the healthy volunteers of Nanjing. In the septic shock group (13 cases) in the Xining, the volume of TVD (5.44 ±1.94 mm/mm2), PVD (4.18 ±1.61 mm/mm2), PPV (42.14%±5.38%) and MFI (1.05 ±0.32) compared with the volume of the healthy volunteers in Xining, the TVD (15.59 ±2.58 mm/mm2), PVD (5.58 ±2.58 mm/mm2), PPV (96.60% ±4.63%) and MFI (2.17 ±0.30) were significant lower (P<0.05). In the healthy volunteers compare with septic shock group in Nanjing area, the TVD(6.80±1.72 vs 10.00±2.10, P<0.05), PVD(5.86±1.58 vs10.81±2.38,P<0.05), PPV(45.42±4.86 vs 84.24±4.86, P<0.05), MFI(1.28±0.28 vs 3.21±0.34 P<0.05), there was significant decreased. In the septic shock group in the Xining compared with the septic shock in Nanjing, there was no significant difference. 10 of 13 patients with septic shock were survived in Xining. 13 of 16 patients with septic shock were survived in Nanjing. CONCLUSIONS: The changes of physiological and pathophysiological characteristic in microcirculation induced by hypoxia would be useful for clinical treatment of septic shock at high altitude.

Fabrication and up-conversion luminescence of Er3+:Y2O3 nanoparticles.

Y2O3 nanoparticles doped with different concentrations of Er3+ were prepared by the co-precipitation method. X-ray diffraction and transmission electron microscopy results show that Er3+ dissolves completely in the Y2O3 cubic phase. The Er3+:Y2O3 nanoparticles are homogeneous in size and nearly spherical, and the average diameter of the particles after being calcined at 1,000 degrees C for 2 h is in the range of 40-60 nm. When Er3+:Y2O3 nanoparticles are excited under a 980 nm diode laser, there are two main emission bands: green emission centered at 562 nm corresponding to the 4S3/2/2H11/2 --> 4115/2 radiative transitions and red emission centered at 660 nm corresponding to the 4F9/2 --> 4I15/2 radiative transitions. By changing the doping concentration of Er3+ ions, the up-conversion luminescence can be gradually tuned from green to red.

Overexpression of pulmonary surfactant protein A like molecules in inflammatory bowel disease tissues / 中南大学学报(医学版)

OBJECTIVE@#To investigate the distribution of pulmonary surfactant protein A (SP-A) like molecules and the bridge of frontier host defense and adaptive immune response cell of CD68 positive macrophages in inflammatory bowel disease (IBD).@*METHODS@#Surgical specimens derived from involved areas and normal area of the colon with Crohn disease (CD) and ulcerative colitis (UC) were obtained from Department of Pathology, Rhode Island Hospital, Brown University Medical Center. The distribution of SP-A like molecule in intestine of IBD was detected by immunohistochemistry.@*RESULTS@#SP-A like molecule located in epithelia of intestine, the surface of intestine villi, blood vessels of connective tissue, and some inflammatory cells. The number of macrophages with both SP-A like molecule and CD68 positive was dramatically increased in the inflammatory area than the normal area. Some CD68 positive macrophages expressed SP-A like immunoreactivity by immunofluorescence double labeling.@*CONCLUSION@#SP-A is an important host defense molecule in lung, and SP-A expression in large intestine may reflect a close relation between 2 organs in immune response towards inflammation.