RESUMO
Three phospholipids (4-6) and three aromatic amines (1-3) were obtained from the methanol extract of Bombycis corpus. Based on spectral data, their structures have been elucidated as nicotiamide (1), cytidine (2), adenine (3), 1-O-(9Z-octadecenoyl)-2-O-(8Z,11Z-octadecadienoyl) sn-glycero-3-phosphorylcholine (4), 1,2-di-O-hexadecanoyl-sn-glycero-3-phosphorylcholine (5) and 1,2-di-O-9Z-octadecenoyl-sn-glycero-3-phosphorylcholine (6). We examined the effects of compounds on synthesis of NGF in cultured astrocytes. By RT-PCR analysis, expresison of NGF mRNA in astrocytes cultured in serum-starvation increased after the addition of phospholipid (10 microM). The NGF content in the culture medium was significantly increased by compound 5, compared with the control value. These results suggest that three phospholipid compounds isolated from the methanol extract of Bombycis corpus may exert neurotrophic effects by stimulation of NGF synthesis in astrocytes.
Assuntos
Fatores de Crescimento Neural/farmacologia , Fosfolipídeos/farmacologia , Preparações de Plantas/farmacologia , Animais , Fatores de Crescimento Neural/química , Fatores de Crescimento Neural/isolamento & purificação , Células PC12 , Fosfolipídeos/química , Fosfolipídeos/isolamento & purificação , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Extratos Vegetais/farmacologia , Preparações de Plantas/química , Preparações de Plantas/isolamento & purificação , RatosRESUMO
Three new (2-4) and one known (1) sphingolipid were identified in the MeOH extract of Bombycis Corpus 101A. Their structures were elucidated as (4E,2S,3R)-2-N-octadecanoyl-4-tetradecasphingenine (1), (4E,6E,2S,3R)-2-N-eicosanoyl-4,6-tetradecasphingadienine (2), (4E,2S,3R)-2-N-eicosanoyl-4-tetradecasphingenine (3), and (4E,6E,2S,3R)-2-N-docosanoyl-4,6-tetradecasphingadienine (4) on the basis of spectroscopic data. Their neurotrophic effects were evaluated by examining PC12 cell neurite outgrowth.
