Expression and significance of miR-126 and VEGF in proliferative diabetic retinopathy / 中国医师杂志

Objective To investigate the expression and clinical significance of miR-126 and vascular endothelial growth factor (VEGF) in proliferative diabetic retinopathy (PDR).Methods 226 cases of diabetic retinopathy (DR) patients admitted in our hospital were studied,including 110 cases of PDR (group PDR),116 non proliferative diabetic retinopathy (NPDR) (group NPDR).80 patients with diabetes mellitus without retinopathy (NDR) were enrolled in DR group at the same period,another 80 healthy subjects (control group) were selected as control group.The plasma miR-126 level of all subjects was detected by real-time fluorescence quantitative polymerase chain reaction (qRT-PCR).The enzyme linked immunosorbent assay (ELISA) method was used to detect plasma VEGF level.The clinical diagnostic value of miR-126 and VEGF in PDR patients was further analyzed.Results Plasma levels of miR-126 in PDR group,NPDR group and NDR group were lower than those in control group (P ＜ 0.05),PDR group was lower than NPDR group and NDR group (P ＜ 0.05);plasma levels of VEGF in PDR group,NPDR group and NDR group were higher than those in control group (P ＜ 0.05),PDR group was higher than NPDR group and NDR group (P ＜ 0.05).Total cholesterol (TC),triglyceride (TG) and glycated hemoglobin (HbAlc) in PDR group were higher than those in NPDR group and NDR group (P ＜0.05),low density lipoprotein cholesterol (LDL-C) in PDR group,NPDR group and NDR group were higher than those in control group (P ＜ 0.05),and LDL-C in PDR group was higher than that in NPDR group and NDR group (P ＜0.05).High density lipoprotein cholesterol (HDL-C) in PDR group and NDR group was lower than that in control group (P ＜ 0.05),C-reactive protein (CRP) in PDR group,NPDR group and NDR group was higher than that in control group (P ＜ 0.05);plasma miR-126 levels in PDR group were negatively correlated with TC,LDL-C,CRP and HbA1c (P ＜ 0.05),but positively correlated with HDL-C (P ＜ 0.05),and no correlation with TG;the plasma levels of VEGF in PDR patients were positively correlated with TC,TG,LDL-C,CRP and HbAlc (P ＜0.05),but negatively correlated with the expressions of miR126 (r =-0.573,P =0.000);the AUC of miR-126 was 0.861,and when the cut-off value was ＜0.64,the diagnostic sensitivity and specificity were 82.50％,83.64％;the area under curve (AUC) of VEGF was 0.889,and when the cut-off value was ＜ 7.000,the sensitivity and specificity of diagnosis were 82.73％,86.25％;0.847 for the AUC of HbA1c,and the sensitivity and specificity 81.82％,87.50％ respectively.Conclusions Plasma miR-126 is low-expressed and VEGF is high-expressed in PDR patients,there is a negative correlation between the two indexes.They may be involved in the course of PDR through abnormal lipid metabolism and inflammatory reaction and may be a potential biomarker for early diagnosis of PDR.

Synthesis of carboxyl-introduced chitosan with C2 amine groups protected and its use in copper (II) removal.

A route for the formation of carboxyl-introduced chitosan (CI-CS) with C2 amine groups protected (CIAP-CS) was investigated to improve copper (II) adsorption. First, the C2 amine groups of the chitosan (CS) were protected via a Schiff-base reaction by benzaldehyde. Then the product was obtained by the introduction of pyromellitic dianhydride to the C6 hydroxyl groups on CS via epichlorohydrin. The last product was obtained by removing the Schiff base with dilute hydrochloride solution. CI-CS without C2 amine groups protected was directly synthesized as well. The adsorbents were characterized by Fourier transform infrared (FTIR), X-ray diffraction (XRD) and X-ray photoelectron spectrometer (XPS). The adsorption properties for copper (II) were investigated. FTIR spectroscopy and XPS clearly showed that most of the amine groups in CS were converted to -N = CH2 groups after the benzaldehyde treatment and that no cross-linking reactions with CS were involved; the HCl treatment after the cross-linking reaction effectively released nitrogen atoms protected into the form of the primary amine again. The results confirm that the CIAP-CS cross-linked with the new method had significantly greater adsorption capacities than the CI-CS cross-linked directly with CS. Mechanism study revealed that the increased adsorption performance is attributed to the large number of primary carboxyl and amine groups available on the surfaces of the CIAP-CS. The adsorption mechanism is based on ion exchange and chelating action, and the adsorption process is mainly chemisorption.

Effect of HNO3 concentration on a novel silica-based adsorbent for separating Pd(II) from simulated high level liquid waste.

A new kind of silica-based (Crea + TODGA)/SiO2-P adsorbent with high selectivity adsorption for palladium (Pd) was synthesized to examined the applicability for partitioning process of high level liquid waste (HLLW). Adsorption behavior of Pd(II) towards (Crea + TODGA)/SiO2-P adsorbent and stability of adsorbent against HNO3 solution were investigated by batch method. The degradation parts of (Crea + TODGA)/SiO2-P dissolved in liquid phase were estimated by total organic carbon (TOC) analyzer. (Crea + TODGA)/SiO2-P adsorbent showed good selectivity adsorption for Pd(II) and reached equilibrium within 24 hr. The adsorption ability of (Crea + TODGA)/SiO2-P for Pd(II) and the content of TOC leaked decreased with the increasing of HNO3 concentration. In 3 M HNO3, the average of K d values were 85.03 cm3/g and 26.10 cm3/g after contact time one to 28 days at 298 K and 323 K, respectively. While the content of TOC leaked from the adsorbent after 28 days were 1095 ppm (298 K) and 2989 ppm (323 K), respectively. Therefore, the adsorbent showed good stability at 298 K after contact with nitric acid for a long time. All results indicated (Crea + TODGA)/SiO2-P can be proposed as an applicable and efficient absorbent for separation of Pd(II) in 3 M HNO3 at 298 K.