Effect of the treatment stage on the serum levels of selected cytokines and antioxidant enzymes in patients with tumors of the reproductive organs.

OBJECTIVE: Chronic inflammation along with concomitant oxidative stress contributes to an increased risk of cancer development. The aim of this study was to analyze selected cytokines and antioxidant enzymes in patients with ovarian and endometrial cancers, taking into account the stage of oncological treatment. PATIENTS AND METHODS: The study sample included 52 female patients with advanced endometrial cancer (n = 26.50%) and ovarian cancer (n = 26.50%), undergoing chemotherapy. Long-term observation at four time points was used in the subjects. Each of the women was blood sampled several times (before surgery, and then before the first, third, and sixth cycle of chemotherapy) in order to determine serum levels of pro- and anti-inflammatory cytokines and antioxidant enzymes. RESULTS: The levels of catalase (CAT), glutathione reductase (GR), interleukin (IL)-10, IL-1α, IL-4 differed significantly depending on the stage of therapy and the type of cancer. The serum levels of IL-4 and IL-10 in patients with ovarian cancer were statistically significantly higher than those observed in patients with endometrial cancer. The activity of the tested antioxidant enzymes varied depending on the chemotherapy cycle. Their highest activity in most cases was observed before the third cycle of chemotherapy, and it decreased before the sixth cycle, irrespective of the type of cancer. CONCLUSIONS: In the studied group of patients with ovarian and endometrial cancer, the applied chemotherapy significantly changed the concentration and activity of some interleukins and antioxidant enzymes. The type of tumor determined the levels of IL-4 and IL-10 before the treatment. Evaluation of inflammatory parameters and oxidative stress in women with cancer of the reproductive organ may help to understand physiological changes resulting from the applied therapy.

Diagnostic markers of insulin resistance to discriminate between prediabetes and diabetes in menopausal women.

OBJECTIVE: Menopause is an important transition period in a woman's reproductive life during which hormonal changes occur, resulting in an increased risk of cardiovascular disease and type 2 diabetes. In this study, we assessed the possibility of using surrogate measures of insulin resistance (IR) to predict the risk of insulin resistance in perimenopausal women. PATIENTS AND METHODS: The study involved 252 perimenopausal women living in the West Pomeranian Voivodeship. The methods employed in this study were diagnostic survey based on the original questionnaire, anthropometric measurement, and laboratory tests performed to determine the levels of selected biochemical parameters. RESULTS: In the entire study population, the highest area under the curve was found for the homeostasis model assessment-insulin resistance (HOMA-IR) and quantitative insulin sensitivity check index (QUICKI). Triglyceride-glucose index (TyG index) showed a higher diagnostic value as a distinction tool between prediabetes and diabetes in perimenopausal women than the other markers. HOMA-IR significantly positively correlated with fasting blood glucose (r = 0.72; p = 0.001), glycated hemoglobin (HbA1C, r = 0.74; p = 0.001), triglycerides (TG, r = 0.18; p < 0.005), and systolic blood pressure (SBP, r = 0.15; p= 0.021), and negatively with high-density lipoprotein (HDL, r = -0.28; p = 0.001). QUICKI negatively correlated with fasting blood (r = -0.051; p = 0.001), HbA1C (r = -0.51; p = 0.001), TG (r = -0.25; p = 0.001), low-density lipoprotein (LDL, r = -0.13; p= 0.045), and SBP (r = -0.16; p = 0.011), and positively with HDL (r = 0.39; p = 0.001). CONCLUSIONS: Anthropometric and cardiometabolic parameters were found to significantly correlate with IR markers. HOMA-beta, the McAuley index (McA), visceral adiposity index (VAI), and lipid accumulation product (LAP) may be useful as predictors of pre-diabetes and diabetes in postmenopausal women.

Self-efficacy and fatigue perceived by nursing students in Poland, Spain and Slovakia during the COVID-19 pandemic.

OBJECTIVE: The two-year long epidemic situation around the world has resulted in an increasing number of people experiencing "pandemic fatigue". The aim of this study was to examine the correlation between a sense of self-efficacy and fatigue experienced in everyday life by nursing students in Poland, Spain and Slovakia during the COVID-19 pandemic. PATIENTS AND METHODS: The study was conducted by the diagnostic survey method on a group of 756 nursing students of the first-cycle degree program, from 20 March to 15 May 2021, in the University of Warmia and Mazury in Olsztyn, Pomeranian Medical University in Szczecin (Poland), as well as in Murcia University (Spain) and in the Catholic University in Ruzomberok (Slovakia). RESULTS: The sense of self-efficacy was significantly lower among the students in Poland than in those in Spain (p < 0.0001) and in Slovakia (p < 0.0001). Students in Slovakia showed symptoms of general, physical, cognitive and psychosocial fatigue much less frequently than those in Poland (p < 0.001) and Spain (p < 0.001). Especially students in Spain acutely felt physical health issues as a result of COVID-19 pandemic-imposed restrictions. CONCLUSIONS: A high level of a generalized sense of self-efficacy among the nursing students participating in the study was identified. However, it was significantly differentiated with respect to the country of residence. The fatigue severity experienced by the students during the COVID-19 pandemic was determined by a sense of self-efficacy.

Comparison of the risk of cardiovascular diseases, stroke, and diabetes among the selected group of football referees and the group of general population men from Northern Poland - a pilot study.

OBJECTIVE: We aimed at comparing the total body fat and visceral adipose tissue content in football referees and in the control group of general population men. An assessment of compliance with health promoting behavior in both groups was carried out. PATIENTS AND METHODS: This study, conducted in Northern Poland, involved 112 men. The study group comprised 56 men, football referees. The control group consisted of randomly chosen general population men, not engaged in any sport activities. Assessment of compliance with health promoting behavior among football referees and general population men was based on ultrasound imaging using the BodyMetrix System device (IntelaMetrix, Poland). The study employed a survey questionnaire comprised of the original section and two standardized questionnaires: the Health Behavior Inventory (HBI) and the NEO-Five Factor Inventory (NEO-FFI): the Health Behavior Inventory (HBI) and the NEO-Five Factor Inventory (NEO-FFI). RESULTS: The visceral adipose tissue content in the study group (football referees) was found to be low, and the excess of body fat was 0-0.25 kg. In the control group, the trunk fat volume was found to be higher by more than 8% as compared with the study group. Also, the level of visceral adipose tissue was high, and the excess of body fat was 0-4 kg. CONCLUSIONS: Thanks to properly planned and systematically continued physical activity, despite non-compliance with certain pro-health principles (increased sweet supply and consumption of alcoholic beverages), football referees are characterized by the correct body fat volume and low level of visceral adipose tissue. The parameters were found to be markedly higher in the control group of randomly selected men from the general population. The risk of diabetes, stroke, and cardiovascular diseases among football referees was found to be very low.

Summary of five years of operation of the out-of-court system for pursuing patient claims in Poland - a retrospective analysis of applications for evaluating medical events.

OBJECTIVE: Providing health services involves a risk of medical events and adverse events. The transparency and quality of the healthcare system have a direct impact on patient's safety. One of the measures of the quality of health services is monitoring and reporting these irregularities, as well as analysing the causes of their occurrence. The aim of this study was to present the principles of the functioning of the Regional Commission for Evaluation of Medical Events in Szczecin and to analyse medical events in the West Pomeranian Voivodeship from 2012 to 2017. MATERIALS AND METHODS: The analysis included applications for evaluating medical events and documentation collected for the purpose of conducting cases by the Regional Commission for Evaluation of Medical Events in Szczecin. The study was retrospective. All applications for evaluating medical events that were received by the Regional Commission for Evaluation of Medical Events in Szczecin in 2012-2017 were analysed. The study was conducted from October 2017 to December 2018. RESULTS: The retrospective analysis of the years 2012-2017 revealed 42 medical events and 120 adverse events. The most common medical events were health disorders (33.3%) and bodily injuries (30.9%). Out of the 42 medical events, 34 (80.9%) were for surgical procedures and childbirth. The most common procedures were orthopedic (26.6%) and surgical (23.5%) procedures. CONCLUSIONS: Medical events and adverse events should be reported so that they can be analyzed, conclusions can be drawn, and remedial measures can be introduced.

Prevalence of subclinical uterine pathologies diagnosed by biopsy and cytological and bacteriological findings in cyclic bitches.

The study was performed on 45 bitches in different cycle phases that were divided into the following groups: anoestrus (I, n=15), heat (pro-oestrus (n=7) or estrous (n=8) (II, n=15) and metestrus (III, n=15). Moreover, all experimental dogs were grouped according to their age: younger than 5 years (Y, n=35) and older than 5 years (O, n=10). The endometrial status was evaluated using cytological, bacteriological and biopsy samples obtained after ovariohysterectomy. The main uterine pathology diagnosed by biopsy was endometritis, since 40%-66% of bitches, independent of the experimental group, developed this condition. No significant differences were found among the cycle phase groups (p>0.05). By contrast, significant differences were found in the age groups; the prevalence of this pathology was higher in older bitches (p=0.0019). The general prevalence of cystic endometrial hyperplasia (CEH) and a normal endometrium (NE) was lower (6.7-26.7% vs 26.7-53.3%) in all groups, and no statistically significant differences were found between certain groups (p>0.05). The percentage of polymorphonuclear neutrophilic leukocytes (PMNs) in endometrial cytology was generally low (⟨ 2%) and did not differ significantly among the experimental groups (p=0.142). In general, a low degree of correlation was found between the diagnostic results by endometrial cytology and biopsy (Kappa Coefficient= 0.046). Positive bacteriological findings were found in approximately 50% of the bitches, independent of the cycle phase and health status of the endometrium. No correlation was found between the bacteriological and histopathological findings (p=0.883). In conclusion, uterine cytology is not a reliable diagnostic method to detect the subclinical inflammatory and degenerative uterine pathologies in cyclic bitches.

Stenotrophomonas maltophilia isolated from prostatic fluid as an infertility factor in a male dog.

The aim of this case was to describe very rare infection of canine prostate gland with Stenotrophomonas maltophilia which had influence on male fertility. The bacterium was cultured from third fraction of the ejaculate collected by manual manipulation. The sperm concentration and motility parameters were evaluated by Hamilton-Thorne Sperm Analyser, version IVOS 12.3, sperm morphology by Diff-Quick staining and live/dead spermatozoa by eosin/nigrosin staining. After 3 weeks of treatment with targeted antibiotic, ciprofloxacin, there was no bacterial growth in prostate fluid. Semen parameters were improved after 60 days from the end of treatment, and females were successfully mated.

The role of the saliva antioxidant barrier to reactive oxygen species with regard to caries development.

OBJECTIVES: The aim of this study was to evaluate the role of the antioxidant barrier in the saliva of children with caries, and its impact on the colonization of cariogenic bacteria. METHODS: This is a cross-sectional study of 81 children aged 1-5 years. Antioxidant levels and salivary bacterial profiles were measured. Patients were divided into two groups as follows: initial stage decay, termed non-cavitated (1-2 in International Caries Detection and Assessment System (ICDAS)), and extensive decay, termed cavitated lesions (5-6 in ICDAS). The control group includes children without caries. RESULTS: The linear regression model demonstrated that the GSH, GSSG, GSH/GSSG, and total antioxidant capacity levels are influenced (P < 0.05) by: the stage of caries and the dominant bacterial strain. Compared with the other groups (P < 0.001), the highest antioxidant parameters were recorded in the saliva of patients with cavitated lesions. DISCUSSION: Our results indicate that the high levels of antioxidants in saliva increase significantly in children in line with the salivary cariogenic bacterial profiles and caries progression.

Occurrence of climacteric symptoms in postmenopausal women after prophylactic bilateral ovariectomy.

PURPOSE OF INVESTIGATION: To analyse the quality of life in postmenopausal women after prophylactic bilateral ovariectomy depending on the time from menopause. MATERIALS AND METHODS: The study involved 252 postmenopausal women grouped according to the time from last menstruation: one to five years (group A), five to ten years (group B), and > ten years (group C). All women were ovariectomized during laparotomy performed for benign diseases of the uterus. Climacteric symptoms were measured with the Kupperman Index one day before and three months after surgery. RESULTS: Highly significant age differences and no substantial BMI differences were demonstrated among the study groups. Before and after surgery climacteric symptoms were reported by 17.06% and 57.8% of women, respectively. After surgery, group A women significantly more often had hot flushes, sweating, nervousness, and sleep disorders, the women in group B significantly more often reported sleep disorders, nervousness, and sweating, and the women in group C significantly more often complained of nervousness. CONCLUSION: In postmenopausal women, ovaries play the most important role during the first ten years from the last menstruation.

Equine Chorionic Gonadotropin and Human Chorionic Gonadotropin Stimulation Increase the Number of Luteinized Follicles and the Progesterone Level Compared with Cabergoline Stimulation in Anoestrus Bitches.

In this study, ovarian morphologies and blood progesterone concentrations following oestrous induction in bitches were examined. Fifty-three clinically healthy anoestrus bitches received cabergoline at a daily dose of 5 µg/kg of body weight per os for 21 days (group I) or subcutaneous equine chorionic gonadotropin at a dose of 20 IU/kg of body weight for five consecutive days with an additional 500 IU s.c. per bitch of human chorionic gonadotropin on the last day of treatment (group II). Twenty bitches that spontaneously displayed oestrous signs were left untreated and served as controls (group III). The induced oestrous rates and ovulation rates in groups I and II were 60.0% vs 64.3% and 86.7% vs 83.3%, respectively. Morphological assessments of the ovarian structures after ovariohysterectomy revealed an increase in the number of luteinized follicles and cysts in group II compared with the two other groups (p < 0.001). In contrast, the numbers of corpora lutea and follicles were similar in all groups. In accordance with the above-mentioned alteration, the progesterone concentration in the gonadotropin group (II) was increased (p < 0.001) in the periovulatory period compared with the other two groups. During the entire sampling period, the progesterone profiles in the cabergoline (I) and control (III) groups were similar and typical of normally cycling bitches. In conclusion, gonadotropin treatment is associated with an increased progesterone level during the periovulatory period that probably originates from luteinized follicles, whereas cabergoline treatment induces cycles with both physiological progesterone concentrations and ovarian morphologies.

Formation of the early canine CL and the role of prostaglandin E2 (PGE2) in regulation of its function: an in vivo approach.

The mechanisms governing corpus luteum (CL) function in domestic dogs remain not fully elucidated. The upregulated expression of cyclooxygenase 2 and prostaglandin (PG) E2 synthase (PGES) at the beginning of the canine luteal phase indicated their luteotrophic roles, and the steroidogenic activity of PGE2 in the early canine CL has been confirmed in vitro. Recently, by applying a cyclooxygenase 2 (COX2)-specific inhibitor (firocoxib [Previcox]; Merial) from the day of ovulation until the midluteal phase, the luteotrophic effects of PGs have been shown in vivo. This is a follow-up study investigating the underlying endocrine mechanisms associated with the firocoxib-mediated effects on the canine CL. Experimental groups were formed with ovariohysterectomies performed on Days 5, 10, 20, or 30 of firocoxib treatments (10 mg/kg bw/24h; TGs = treated groups). Untreated dogs served as controls. A decrease of steroidogenic acute regulatory (STAR) protein expression was observed in TGs. The expression of PGE2 synthase was significantly suppressed in TGs 5 and 10, and both PGE2 and PGF2α levels were decreased in luteal homogenates, particularly from CL in TG 5. Similarly, expression of the prolactin receptor (PRLR) was diminished in TGs 5 and 20. The expression of PGE2 receptors PTGER2 (EP2) and PTGER4 (EP4), the PG- transporter (PGT), and 15-hydroxy PG dehydrogenase (HPGD) was not affected in TGs. Our results substantiate a direct luteotrophic role of PGs in the early canine CL, i.e., by upregulating the steroidogenic machinery. Additionally, the possibility of an indirect effect on PRL function arises from the increased prolactin receptor expression in response to PGE2 treatment in canine lutein cells observed in vitro.

Progesterone level does not distinguish the different course of canine ovulation determined by ultrasonography.

The aim of this study was to compare progesterone concentrations in bitches during ovulation with a different course of follicular collapse as determined by ultrasonography. The study was conducted on 36 animals of different breeds, from 2 to 7 years old, and from 2 to 44 kg of body weight. Ultrasound examinations of ovaries were conducted in the peri-ovulatory period until ovulation was detected. Based on USG scans the animals were divided into two groups: group A with fast follicular collapse within 1 day, and group B with gradual follicular collapse within 2-3 days. Progesterone measurements were performed in both groups on the day when the beginning of ovulation was diagnosed by USG. Follicular collapse in group A was observed in 27.8% of animals (n = 10) and in group B in 72.2% (n = 26). There were no statistical differences (p < 0.05) in progesterone concentrations between groups A and B (6.68 +/- 0.92 ng/ml and 6.81 +/- 0.57 ng/ml respectively). From 31 bred bitches (natural mating or insemination) pregnancy was confirmed in 29 females (93.5%). These results provide information about the sufficient stability of progesterone concentration during ovulation regardless of the follicular collapse speed. Ultrasonography during the peri-ovulatory period combined with progesterone measurements allows progress in ovulation management.

The severity of depressive symptoms vs. serum Mg and Zn levels in postmenopausal women.

The aim of this study was to assess the severity of depressive symptoms in postmenopausal women, depending on serum Mg and Zn levels. The study involved 171 postmenopausal women from Poland, who were not using menopausal hormone therapy (MHT). The intensity of depressive symptoms was evaluated using a standard research technique, the Beck Depression Inventory (BDI). The plasma Mg and Zn concentrations were measured. Depressive symptoms of different severity levels were diagnosed in 36.8 % of the women. The mean serum Mg level was 1.53 ± 0.28 mg/dL, and Zn level was 72 ±14 µg/dL. The women with higher serum Mg and Zn levels had less depressive symptoms, and this observation is a precious information which can be used when planning depressive disorder prevention programmes.

The virulence of Streptococcus mutans and the ability to form biofilms.

In some diseases, a very important role is played by the ability of bacteria to form multi-dimensional complex structure known as biofilm. The most common disease of the oral cavity, known as dental caries, is a top leader. Streptococcus mutans, one of the many etiological factors of dental caries, is a microorganism which is able to acquire new properties allowing for the expression of pathogenicity determinants determining its virulence in specific environmental conditions. Through the mechanism of adhesion to a solid surface, S. mutans is capable of colonizing the oral cavity and also of forming bacterial biofilm. Additional properties enabling S. mutans to colonize the oral cavity include the ability to survive in an acidic environment and specific interaction with other microorganisms colonizing this ecosystem. This review is an attempt to establish which characteristics associated with biofilm formation--virulence determinants of S. mutans--are responsible for the development of dental caries. In order to extend the knowledge of the nature of Streptococcus infections, an attempt to face the following problems will be made: Biofilm formation as a complex process of protein-bacterium interaction. To what extent do microorganisms of the cariogenic flora exemplified by S. mutans differ in virulence determinants "expression" from microorganisms of physiological flora? How does the environment of the oral cavity and its microorganisms affect the biofilm formation of dominant species? How do selected inhibitors affect the biofilm formation of cariogenic microorganisms?

The pathogenicity of the Streptococcus genus.

Streptococcus infections are still one of the important problems facing contemporary medicine. As the World Health Organization (WHO) warns, Streptococcus pneumoniae is responsible for the highest number of pneumonia cases all over the world. Despite an increasing number of pneumococcal vaccinations, incidences of disease connected to this pathogen's infection stay at the same level, which is related to a constantly increasing number of infections caused by nonvaccinal serotypes. Unfortunately, the pathogenicity of bacteria of the Streptococcus genus is also connected to species considered to be physiological flora in humans or animals and, additionally, new species exhibiting pathogenic potential have been discovered. This paper presents an opinion concerning the epidemiology of streptococci infections based on case studies and other publications devoted to this problem. It also sheds new light based on recent reports on the prevention of protective vaccinations application in the case of streptococci infections.

Oral folic acid supplementation decreases palate and/or lip cleft occurrence in Pug and Chihuahua puppies and elevates folic acid blood levels in pregnant bitches.

The aim of this study was to compare the frequency of the occurrence of lip and/or palate cleft (CL/CP) in new-borns of two breeds, Pugs and Chihuahuas, and to measure the folic acid blood levels in bitches during gestations both with and without folic acid oral supplementation. Bitches of 13 Pugs and 17 Chihuahuas with CL/CP cases were used in the study. In trial 1, the animals of the experimental group (n=25) were given additional folic acid from the onset of heat till the 40th day of gestation. The females of the control group (n=12) were fed a traditional diet. From all the animals blood was collected at the onset of heat, 14 days later and on the 30th day of the gestation to estimate folic acid concentration. In trial 2, the prevalence of CP/CL cases in litters from pregnancies before and after supplementation was compared. The percentage of puppies with CL/CP after supplementation decreased in both Pugs and Chihuahua puppies (10.86% and 15.78% vs. 4.76% and 4.8% respectively). On Day 0, the concentrations of folic acid were at a low physiological level (around 8 ng/ml) in all the animals. In bitches of the experimental group the blood level of folic acid on day 14th and 30th of the treatment showed an increase in both breeds (13.65 +/- 4.27 ng/ml in Pugs, 10.79 +/- 2.84 ng/ml in Chihuahuas, and 14.94 +/- 3.22 ng/ml in Pugs, 12.95 +/- 3.58 in Chihuahuas, respectively) while in the control group, this level decreased with time of gestation both in Pugs and in Chihuahuas (around 6 ng/ml). Folic acid supplementation seems to be a simple, effective preventive method to reduce the risk of CL/CP, especially in the predisposed breeds.

Clinical forms of infections in neonates hospitalized in clinic of obstetrics and perinatology within the space of one year.

Because of their specificity, infections in neonatal units form one of the main clinical problems. Our research involved all neonates (1019) hospitalized in Clinic of Obstetrics and Perinatology within the space of one year. Clinically manifested infections were diagnosed in the total number of 47 (4.6%) newborns, including 23 (2.4%) neonates from the neonatal unit (NU) and 24 (46.2%)--from the Neonatal Intensive Care Unit (NICU). In both units, the most commonly observed were general infections (59.6%) and pneumonias (21.3%); cerebrospinal meningitis and necrotic enteritis were diagnosed in a few cases. Urinary system infections were only found in neonates hospitalized in the NU (30.5%). The course of infection was mild in most cases.

Clinical and microbiological characteristics of hospital infections in the neonatal intensive care unit.

Neonates hospitalized in intensive care units, are exposed to a higher risk of infectious complications. The research involved 52 neonates hospitalized in the Neonatal Intensive Care Unit (NICU), Chair and Clinic of Obstetrics and Perinatology over a span of one year. The incidence of hospital infections as well as etiological factors were analyzed. Clinically manifested hospital infections were diagnosed in 38.5% of babies with very low or extremely low birth weight, in boys twice as often as in girls. Generalised invasive infections prevailed; in most cases they were caused by Gram-negative rods, mainly Klebsiella spp.

Comparative analysis of informative support in lactation in lying-in women hospitalized in rooming-in system.

PURPOSE: The aim of the study was to assess the expected and received informative support in lactation in hospitalized lying-in women. Such variables as the number of deliveries and participation in antenatal classes were taken into consideration. MATERIAL AND METHODS: The research was conducted from May to September 2005, and involved 202 lying-in women staying in maternity wards in Chair and Clinic of Obstetrics and Perinatology, Pomeranian Medical University (PAM) in Szczecin, and Obstetrics and Gyneacology Unit in Independent Public Specialistic Health Care Centre Zdroje Szczecin. The diagnostic survey was carried out; it was based on the questionnaire of author's design. RESULTS: The obtained results suggest that primiparas significantly more frequently than multiparas show demand for all elements of informative support in lactation (p < 0.001), while women who did not attend antenatal classes considerably more often need information on the half of elements of informative support connected with lactation. Informative support that lying-in women receive does not satisfy the demand for it. CONCLUSIONS: 1. Professional support provided by midwives/nurses should be particularly directed on primiparas and women who did not attend antenatal classes. 2. Participation of future parents in antenatal classes causes them to be better prepared to breastfeeding, and be less needing the mentioned information while their stay in a mother-baby ward. 3. It is necessary that midwives/nurses constantly perfect their professional skills in order to improve the quality of obstetrical care.

Estimation of the declared knowledge of anaesthesiology nurses concerning some chosen procedures and actions being within the scope of professional competence.

Together with introducing legal regulations as well as modem standards of postgraduate education, the professional position of a nurse has strengthened and also her responsibility for professional activity has increased. Performing the profession of an anaesthesiology nurse involves necessity of possessing some adequate professional qualifications. The aim of the study was an attempt to answer the question on how the knowledge of an anaesthesiology nurse concerning the activities being within her professional qualifications shapes. The research was carried out in 2005 among 123 anaesthesiology nurses working in some Polish hospitals chosen at random. Method of diagnostic opinion poll was applied and a questionnaire of own authorship was a research tool. Analysing the obtained results the following conclusions have been drawn: 1. The level of knowledge of an anaesthesiology nurses on the activities being within their qualifications is unsatisfactory; 2. Obtaining competence in anaesthesiology nursing involves the necessity of continual raising of qualifications by nurses, especially being on specialization level; 3. A necessity of differentiation between particular competence levels of anaesthesiology nurses during different forms of education should be underlined; 4. Possibility of realization of their competences on a particular level by anaesthesiology nurses is connected with the necessity of improving whole therapeutic team work.