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1.
ACS Omega ; 8(19): 17017-17027, 2023 May 16.
Artigo em Inglês | MEDLINE | ID: mdl-37214735

RESUMO

A detailed understanding of the cell adhesion on polymeric surfaces is required to improve the performance of biomaterials. Quartz crystal microbalance with dissipation (QCM-D) as a surface-sensitive technique has the advantage of label-free and real-time monitoring of the cell-polymer interface, providing distinct signal patterns for cell-polymer interactions. In this study, QCM-D was used to monitor human fetal osteoblastic (hFOB) cell adhesion onto polycaprolactone (PCL) and chitosan (CH) homopolymer films as well as their blend films (75:25 and 25:75). Complementary cell culture assays were performed to verify the findings of QCM-D. The thin polymer films were successfully prepared by spin-coating, and relevant properties, i.e., surface morphology, ζ-potential, wettability, film swelling, and fibrinogen adsorption, were characterized. The adsorbed amount of fibrinogen decreased with an increasing percentage of chitosan in the films, which predominantly showed an inverse correlation with surface hydrophilicity. Similarly, the initial cell sedimentation after 1 h resulted in lesser cell deposition as the chitosan ratio increased in the film. Furthermore, the QCM-D signal patterns, which were measured on the homopolymer and blend films during the first 18 h of cell adhesion, also showed an influence of the different interfacial properties. Cells fully spread on pure PCL films and had elongated morphologies as monitored by fluorescence microscopy and scanning electron microscopy (SEM). Corresponding QCM-D signals showed the highest frequency drop and the highest dissipation. Blend films supported cell adhesion but with lower dissipation values than for the PCL film. This could be the result of a higher rigidity of the cell-blend interface because the cells do not pass to the next stages of spreading after secretion of their extracellular matrix (ECM) proteins. Variations in the QCM-D data, which were obtained at the blend films, could be attributed to differences in the morphology of the films. Pure chitosan films showed limited cell adhesion accompanied by low frequency drop and low dissipation.

2.
Biomed Mater Eng ; 34(5): 459-472, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37005873

RESUMO

BACKGROUND: Magnesium (Mg) enhances the bone regeneration, mineralization and attachment at the tissue/biomaterial interface. OBJECTIVE: In this study, the effect of Mg on mineralization/osseointegration was determined using (Ti,Mg)N thin film coated Ti6Al4V based plates and screws in vivo. METHODS: TiN and (Ti,Mg)N coated Ti6Al4V plates and screws were prepared using arc-PVD technique and used to fix rabbit femur fractures for 6 weeks. Then, mineralization/osseointegration was assessed by surface analysis including cell attachment, mineralization, and hydroxyapatite deposition on concave and convex sides of the plates along with the attachment between the screw and the bone. RESULTS: According to Scanning Electron Microscopy (SEM) and Energy Dispersive Spectroscopy (EDS) analyses; cell attachment and mineralization were higher on the concave sides of the plates from both groups in comparison to the convex sides. However, mineralization was significantly higher on Mg-containing ones. The mean gray value indicating mineralized area after von Kossa staining was found as 0.48 ± 0.01 and 0.41 ± 0.04 on Mg containing and free ones respectively. Similarly, Fourier Transform Infrared Spectroscopy (FTIR) and X-ray diffraction (XRD) analyses showed that hydroxyapatite growth was abundant on the Mg-containing and concave sides of the plates. Enhanced mineralization and strong attachment to bone were also detected in EDS and SEM analyses of Mg-containing screws. CONCLUSION: These findings indicated that (Ti,Mg)N coatings can be used to increase attachment at the implant tissue interface due to accelerated mineralization, cell attachment, and hydroxyapatite growth.


Assuntos
Magnésio , Titânio , Animais , Coelhos , Magnésio/farmacologia , Titânio/química , Materiais Revestidos Biocompatíveis/química , Osseointegração , Durapatita/química , Microscopia Eletrônica de Varredura , Fêmur/cirurgia , Propriedades de Superfície
3.
Talanta ; 259: 124471, 2023 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-37001401

RESUMO

The objective of the study is, for the first time, to construct a new near infrared (NIR) fluorophore, spectrophotometric, colorimetric, ratiometric, and turn-on probe (CSME) based on chromenylium cyanine platform decorated with methionine biomolecule to provide an efficient solution for critical shortcoming to be encountered for analysis of hazardous Hg2+ in environment and living cell. The CSME structure and its interaction with Hg2+ ion were evaluated by NMR, FTIR, MS, UV-Vis and fluorescence methods as well as Density Functional Theory (DFT) calculations. The none fluorescence CSME having spirolactam ring only interacted with Hg2+ in aqueous solution including competing ions. This interaction caused the fluorescence CSME with opened spirolactam form which exhibited spectral and colorimetric changes in the NIR region. The probe based on UV-Vis and fluorescence techniques respond in 90 s, has wide linear ranges (for UV-Vis: 6.29 × 10-8 - 1.86 × 10-4 M; for fluorescence: 9.49 × 10-9 - 1.13 × 10-5 M), and has a lower Limit of Detection (LOD) value (for fluorescence: 4.93 × 10-9 M, 0.99 ng/mL) than the value predicted by the US Environmental Protection Agency (EPA) organization. Hg2+ analysis was performed in drinking and tap water with low Relative Standard Deviation (RSD) values and high recovery. Smartphone and living cell applications were successfully performed for colorimetric sensing Hg2+ in real samples and 3T3 cells, respectively.


Assuntos
Corantes Fluorescentes , Mercúrio , Camundongos , Animais , Corantes Fluorescentes/química , Metionina , Água/química , Racemetionina , Mercúrio/análise
4.
ACS Appl Bio Mater ; 6(4): 1594-1603, 2023 04 17.
Artigo em Inglês | MEDLINE | ID: mdl-36922721

RESUMO

Inspired by nature, we designed organohydrogels (OHGs) consisting of a silk fibroin (SF) hydrogel as the continuous phase and the hydrophobic microinclusions based on semicrystalline poly(n-octadecyl acrylate) (PC18A) as the dispersed phase. SF acts as a self-emulsifier to obtain oil-in-water emulsions, and hence, it is a versatile and green alternative to chemical emulsifiers. We first prepared a stable oil-in-water emulsion without an external emulsifier by dispersing the n-octadecyl acrylate (C18A) monomer in an aqueous SF solution. To stabilize the emulsions for longer times, gelation in the continuous SF phase was induced by the addition of ethanol, which is known to trigger the conformational transition in SF from random coil to ß-sheet structures. In the second step, in situ polymerization of C18A droplets in the emulsion system was conducted under UV light in the presence of a photoinitiator to obtain high-strength OHGs with shape-memory function, and good cytocompatibility. The incorporation of hydrophilic N,N-dimethylacrylamide and noncrystallizable hydrophobic lauryl methacrylate units in the hydrogel and organogel phases of OHGs, respectively, further improved their mechanical and shape-memory properties. The shape-memory OHGs presented here exhibit switchable viscoelasticity and mechanics, a high Young's modulus (up to 4.3 ± 0.1 MPa), compressive strength (up to 2.5 ± 0.1 MPa), and toughness (up to 0.68 MPa).


Assuntos
Fibroínas , Fibroínas/química , Seda/química , Emulsões/química , Hidrogéis/química , Água/química
5.
Spectrochim Acta A Mol Biomol Spectrosc ; 287(Pt 2): 122060, 2023 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-36395583

RESUMO

A new dual-channel probe based on rhodamine B derivative (MSB) was successfully designed, synthesized, characterized by Nuclear Magnetic Resonance (NMR) Spectroscopy, Fourier Transform Infrared Spectrophotometer (FTIR), Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS), X-ray Photoelectron Spectroscopy (XPS), and Single Crystal X-rayDiffraction, and the sensing abilities toward Fe3+ cation have been demonstrated and the probe was successfully utilized for fluorescence imaging of Fe3+ in living cells. The probe demonstrated quite fast, sensitive, and selective response to Fe3+ by causing an extreme enhancement in UV-vis and fluorescence spectroscopy techniques in the buffered aqueous media which makes MSB a dual-channel probe. While the color of MSB solution was initially light yellow, it turned pink in the presence of Fe3+, which provided highly selective naked-eye determination among several ions as alkaline, alkaline-earth, and transition metal ions. After that, the probe was easily applied to paper strips and real samples such as drinking waters and supplementary iron tablets for sensing Fe3+ in an aqueous solution. The detection limit (LOD) and the response time of the probe were determined as 4.85x10-9 M and 4 min, respectively, which are quite lower compared with other rhodamine based Fe3+ sensors in the literature. According to Job's plot, 1H NMR titration, MALDI-TOF MS, XPS, and DFT study techniques, the complexation ratio between MSB and Fe3+ was found as 1:1. Moreover, the spectral response was reversible with alternately addition of Fe3+ or Na2EDTA to the MSB solution. In addition, fluorescence imaging in NIH/3T3 mouse fibroblast cells and studies in real samples with a quite high recovery rate exhibited that the probe is qualified for detection of Fe3+ ion with multiple practical usages.


Assuntos
Imagem Óptica , Smartphone , Animais , Camundongos , Rodaminas , Espectroscopia Fotoeletrônica , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
6.
Biotechnol Appl Biochem ; 69(4): 1557-1566, 2022 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34297408

RESUMO

In this work, a biosensor based on surface plasmon field-enhanced florescence spectroscopy (SPFS) method was successfully constructed to detect the truncated form of cholera toxin, that is, its beta subunit (CTX-B). CTX-B is a relatively small molecule (12 kDa) and it was chosen as model analyte for the detection of protein toxins originated from waterborne pathogens. Recognition layer was prepared on gold-coated LaSFN9 glasses modified with 11-mercaptoundecanoic acid (11-MUA). Biotin-conjugated anti-CTX-B polyclonal antibody (B-Ab) was immobilized on streptavidin (SA) layer constructed on the 11-MUA-modified surface. CTX-B amount was determined with direct assay using B-Ab in surface plasmon resonance (SPR) mode and with sandwich assay in SPFS mode using Cy5-conjugated anti-CTX-B polyclonal antibody. Minimum detected CTX-B concentrations were 10 and 0.01 µg/ml with SPR and SPFS, respectively, showing the sensitivity of the SPFS system over the conventional one. The detection was done in 2-6 h, which was faster than both culture and polymerase chain reaction (PCR)-based methods. Stability tests were performed with SA-coated sensors (excluding B-Ab). In this form, the layer was stable after 30 days of storage in phosphate-buffered saline (PBS; 0.01 M, pH = 7.4) at +4°C. B-Ab layer was formed immediately on them before each measurement.


Assuntos
Técnicas Biossensoriais , Toxina da Cólera , Biotina/química , Ouro/química , Análise Espectral , Estreptavidina/química , Ressonância de Plasmônio de Superfície/métodos
7.
Carbohydr Polym ; 271: 118407, 2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34364550

RESUMO

In this study, three natural biomaterials, Locust bean gum (LBG), Xanthan gum (XG), and Mastic gum (MG), were combined to form cryogel scaffolds. Thermal and chemical characterizations revealed the successful blend formation from LBG-XG (LX) and LBG-XG-MG (LXM) polymers. All blends resulted in macro-porous scaffolds with interconnected pore structures under the size of 400 µm. The swollen cryogels had similar mechanical properties compared with other polysaccharide-based cryogels. The mean tensile and compressive modulus values of the wet cryogels were in the range of 3.5-11.6 kPa and 82-398 kPa, respectively. The sustained release of the small molecule Kartogenin from varying concentrations and ratios of cryogels was in between 32 and 66% through 21 days of incubation. Physical, mechanical, and chemical properties make LX and LXM polysaccharide-based cryogels promising candidates for cartilage and other soft tissue engineering, and drug delivery applications.


Assuntos
Criogéis/química , Preparações de Ação Retardada/química , Alicerces Teciduais/química , Anilidas/química , Animais , Sobrevivência Celular/efeitos dos fármacos , Criogéis/toxicidade , Preparações de Ação Retardada/toxicidade , Liberação Controlada de Fármacos , Galactanos/química , Galactanos/toxicidade , Mananas/química , Mananas/toxicidade , Resina Mástique/química , Resina Mástique/toxicidade , Células-Tronco Mesenquimais/efeitos dos fármacos , Camundongos , Células NIH 3T3 , Ácidos Ftálicos/química , Gomas Vegetais/química , Gomas Vegetais/toxicidade , Polissacarídeos Bacterianos/química , Polissacarídeos Bacterianos/toxicidade , Porosidade , Ratos Sprague-Dawley , Engenharia Tecidual/instrumentação , Engenharia Tecidual/métodos
8.
J Biomater Appl ; 35(6): 655-669, 2021 01.
Artigo em Inglês | MEDLINE | ID: mdl-33283583

RESUMO

Bacterial infections and lack of osseointegration may negatively affect the success of titanium (Ti) implants. In the present study, a functional coating composed of chitosan (CS) microspheres and nano hydroxyapatite (nHA) was prepared to obtain antimicrobial Ti implants with enhanced bioactivity. First, the chitosan microspheres were fixed to Ti surfaces activated by alkali and heat treatment, then nHA coatings were precipitated onto these surfaces. Ciprofloxacin was loaded into the microspheres using two different procedures; encapsulation and diffusion. Scanning electron microscopy micrographs of the modified Ti surfaces showed that the coating was successfully deposited onto the Ti surfaces and stable for 30 days in PBS. The drug was completely released from free microspheres loaded by encapsulation in 21 days whereas only 89% release was observed after immobilization. The burst release also decreased from ca. 55% to ca. 35%. The release was further reduced following the nHA precipitation. The modified Ti surfaces showed antimicrobial activity based on the bacterial time-kill assay using S. aureus, but the efficiency was affected by both nHA precipitation and drug loading strategy. Highest antimicrobial activity was seen in the samples without nHA layer, and when the drug was loaded by diffusion. Fourier transform infrared spectroscopy and X-ray diffraction analyses revealed that nHA on the surface enhanced HA growth in simulated body fluid for 3 weeks, showing increased osseointegration potential. Therefore, the proposed coating may be used to prevent Ti implant failure originated from bacterial infection and/or low bioactivity.


Assuntos
Antibacterianos/química , Quitosana/química , Materiais Revestidos Biocompatíveis/química , Sistemas de Liberação de Fármacos por Nanopartículas/química , Titânio/química , Antibacterianos/farmacologia , Quitosana/metabolismo , Materiais Revestidos Biocompatíveis/metabolismo , Liberação Controlada de Fármacos , Durapatita/química , Humanos , Microesferas , Osseointegração , Próteses e Implantes , Staphylococcus aureus/efeitos dos fármacos , Propriedades de Superfície
9.
J Biomed Mater Res B Appl Biomater ; 109(2): 227-237, 2021 02.
Artigo em Inglês | MEDLINE | ID: mdl-32770599

RESUMO

Magnesium (Mg) based implants such as plates and screws are often preferred to treat bone defects because of the positive effects of magnesium in bone growth and healing. Their low corrosion resistance, however, leads to fast degradation and consequently failure before healing was completed. Previously, we developed Mg doped titanium nitrate (TiN) thin film coatings to address these limitations and demonstrated that <10 at% Mg doping led to enhanced mineralization in vitro. In the present study, in vivo performance of (Ti,Mg)N coated Ti6Al4V based plates and screws were studied in the rabbit model. Bone fractures were formed on femurs of 16 rabbits and then fixed with either (Ti,Mg)N coated (n = 8) or standard TiN coated (n = 8) plates and screws. X-ray imaging and µCT analyses showed enhanced bone regeneration on fracture sites fixed with (Ti,Mg)N coated plates in comparison with the Mg free ones. Bone mineral density, bone volume, and callus volume were also found to be 11.4, 23.4, and 42.8% higher, respectively, in accordance with µCT results. Furthermore, while TiN coatings promoted only primary bone regeneration, (Ti,Mg)N led to secondary bone regeneration in 6 weeks. These results indicated that Mg presence in the coatings accelerated bone regeneration in the fracture site. (Ti,Mg)N coating can be used as a practical method to increase the efficiency of existing bone fixation devices of varying geometry.


Assuntos
Ligas/química , Placas Ósseas , Parafusos Ósseos , Materiais Revestidos Biocompatíveis/química , Fraturas do Fêmur/cirurgia , Consolidação da Fratura , Magnésio/química , Titânio/química , Animais , Modelos Animais de Doenças , Masculino , Coelhos
10.
J Biomed Mater Res A ; 108(3): 760-759, 2020 03.
Artigo em Inglês | MEDLINE | ID: mdl-31788940

RESUMO

Hypothyroidism is an autoimmune disease associated with underactive thyroid gland. In this study, a dual effect polymeric system was designed to release Cepharanthine (CEP) to block T cell activation and Selenium (Se) to decrease the anti-thyroid peroxidase (TPOAb) concentration in order to treat hypothyroidism. For this purpose, poly(ethylene-vinyl acetate) (PEVA) and polyethylene glycol (PEG) nanoparticles (NPs) including CEP were synthesized by emulsion solvent evaporation method and they were loaded to polyurethane (PU)/PEG-PUSe-PEG block copolymer blends which were fabricated by particulate leaching technique as porous sponges. Fourier-Transform Infrared (FTIR), Raman, and Nuclear Magnetic Resonance (NMR) analysis showed successful synthesis of PEG-PUSe-PEG block copolymer. A long-term zero-order release profile was obtained for CEP. Se release rate from matrices showed an oxidative stress-mediated release which can be used to adjust Se amount. According to MTS results conducted by NIH 3T3 fibroblasts, both NPs and matrices have no adverse effect on cell viability. Fluorescence microscopy and SEM images confirm the MTS results. The dual release system has potential to be effectively used in long-term treatment of hypothyroidism by addressing both auto-immune response and hormone regulation.


Assuntos
Anti-Inflamatórios não Esteroides/administração & dosagem , Benzilisoquinolinas/administração & dosagem , Preparações de Ação Retardada/química , Hipotireoidismo/tratamento farmacológico , Polietilenoglicóis/química , Selênio/administração & dosagem , Animais , Anti-Inflamatórios não Esteroides/uso terapêutico , Benzilisoquinolinas/uso terapêutico , Camundongos , Células NIH 3T3 , Nanopartículas/química , Poliuretanos/química , Polivinil/química , Selênio/uso terapêutico
11.
Colloids Surf B Biointerfaces ; 176: 18-26, 2019 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-30590345

RESUMO

Supported lipid bilayers (SLB) functionalized with bioactive molecules can be effectively used to study the interaction of cells with different molecules for fundamental research or to develop biosynthetic systems for various biomedical applications. In this study, RGD and Osteocalcin mimetic (OSN) peptides were used as model molecules for functionalization of otherwise passive SLBs to evaluate cell-surface interactions via real-time monitoring in quartz crystal microbalance with dissipation. Similar platforms were also used in cell culture environment. It was seen that low density of mobile RGD peptides on SLB platforms preserved their biological activity and promoted cell adhesion more efficiently than high number of immobile, physisorbed peptides. Even though nonspecific protein and cell attachment was promoted, cells did not spread well on OSN-coated control surfaces. The stability of SLBs produced with different lipids were evaluated in various medium conditions. Enrichment with different lipids increased the stability of SLB to pure PC bilayer.


Assuntos
Bicamadas Lipídicas/química , Peptídeos/química , Adesão Celular , Membrana Celular , Células Cultivadas , Humanos , Oligopeptídeos/química , Imagem Óptica , Osteocalcina/química , Técnicas de Microbalança de Cristal de Quartzo , Propriedades de Superfície
12.
Biomed Mater Eng ; 29(4): 427-438, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30282341

RESUMO

BACKGROUND: In vitro evaluation of cell-surface interactions for hard tissue implants have mostly been done using osteoblasts. However, when an implant is placed in the body, mesenchymal stem cells (MSCs) play a major role in new bone formation. Therefore, using MSCs in cell-surface investigations may provide more reliable information on the prediction of in vivo behavior of implants. OBJECTIVE: In this study, Mg doped TiN coatings ((Ti,Mg)N) were prepared and tested for their effect on MSC differentiation and mineralization. METHODS: MSCs were isolated from rat bone marrow (rBMSCs) and seeded onto bare Ti, TiN and Mg containing (Ti,Mg)N surfaces. Cell proliferation, osteogenic differentiation (collagen type 1, alkaline phosphatase activity), calcium phosphate deposition (von Kossa staining, Scanning Electron Microscopy) analysis were conducted. RESULTS: Differentiation towards osteoblast lineage was significantly improved with the increment in Mg presence. Collagen type I deposition, mineralization, and the ALP activity were higher on high Mg containing (>10 at% Mg) surfaces but differentiation of rBMSCs were found to be delayed. CONCLUSIONS: Mg presence affected rBMSCs proliferation and differentiation positively in a dose-dependent manner. However, high Mg amounts delayed both proliferation and differentiation.


Assuntos
Magnésio , Células-Tronco Mesenquimais/efeitos dos fármacos , Titânio , Animais , Diferenciação Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Colágeno Tipo I/metabolismo , Relação Dose-Resposta a Droga , Magnésio/farmacologia , Teste de Materiais , Células-Tronco Mesenquimais/citologia , Células-Tronco Mesenquimais/metabolismo , Osteoblastos/citologia , Osteogênese/efeitos dos fármacos , Ratos , Engenharia Tecidual , Titânio/farmacologia
13.
Artif Cells Nanomed Biotechnol ; 46(3): 607-618, 2018 May.
Artigo em Inglês | MEDLINE | ID: mdl-28562085

RESUMO

Stem cells of dental origin emerged as a new source for the regeneration of tissues with advantages mainly including non-invasive collection procedures and lack of ethical contraversies with their harvest or use. In this study, porcine TGSCs (pTGSCs) were isolated from mandibular third molar tooth germs of 6-month-old domestic pigs. This is the first study that reports the isolation and characterization of TGSCs from porcine third molars and their differentiation depending on STRO-1 expression. PTGSCs were sorted according to their STRO-1 expression as STRO-1(+) and STRO-1(-). Sorted and unsorted heterogenous cells (US) were characterized by their osteogenic, chondrogenic and adipogenic differentiation capabilities. STRO-1(+) cells exhibited a higher proliferation rate owing to their clonogenic properties. All three groups of cells were found differentiated into osteogenic lineage as shown by ALP activity, calcium deposition assay, detection of osteogenic mRNAs and, proteins and mineralization staining. According to differentiation analysis, STRO-1(+) cells did not show a better performance for osteogenesis compared to STRO-1(-) and US cells. This might indicate that STRO-1(+) cells might require a heterogeneous population of cells including STRO-1(-) in their niche to perform their proposed role in osteogenesis.


Assuntos
Antígenos de Superfície , Osso e Ossos/metabolismo , Dente Molar/metabolismo , Osteogênese , Células-Tronco/metabolismo , Engenharia Tecidual , Animais , Osso e Ossos/citologia , Células Cultivadas , Citometria de Fluxo , Células-Tronco/citologia , Suínos
14.
Biointerphases ; 13(1): 011001, 2017 Dec 12.
Artigo em Inglês | MEDLINE | ID: mdl-29232957

RESUMO

Quartz crystal microbalance with dissipation (QCM-D) is one of the powerful techniques, which allow real time, quantitative and noninvasive analysis of the interaction of different cell types with various modified surfaces. In this study, the dynamic adhesion behavior of human fetal osteoblastic bone (hfOB) cell lines was first monitored on untreated and hydrophilically treated gold sensor surfaces as reference substrates. Adhesion was also observed under light microscopy to facilitate the evaluation. Cells increased their surface contact area and spread more on hydrophilic surfaces, and showed distinct profile with an increased rigidity at the interfacial layer, which is assigned to extracellular matrix remodeling. Further, the adhesion strength and kinetics were characterized on cell adhesive (poly-l-lysine and fibronectin) and repellent (bovine serum albumin) surfaces. The overall results indicated that protein-mediated specific interactions contributed mostly to the dissipation changes (ΔD) or acoustic ratio (ΔD/Δf). Finally, the potential of QCM-D to distinguish healthy and cancerous cells were evaluated by comparing the results of hfOB cells with that of SaOS-2 (osteosarcoma) cancerous cells. Cancerous cells interacted more strongly and showed more viscoelastic characteristic than the healthy cells.


Assuntos
Técnicas Biossensoriais/métodos , Adesão Celular , Técnicas Citológicas/métodos , Osteoblastos/fisiologia , Técnicas de Microbalança de Cristal de Quartzo , Linhagem Celular , Humanos
15.
Colloids Surf B Biointerfaces ; 160: 117-125, 2017 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-28918188

RESUMO

Most of the model lipid membrane studies on gold involve the usage of various surface-modification strategies to rupture liposomes and induce lipid bilayer formation since liposomes with polar surfaces do not interact with bare, hydrophobic gold. In this study, a thiol-modified phospholipid, 1,2-Dipalmitoyl-sn-Glycero-3-Phosphothioethanol (DPPTE) was incorporated into phosphatidylcholine (PC) based liposomes to form supported lipid bilayer (SLB) on gold surfaces without further modification. The binding kinetics of liposomes with different DPPTE ratio (0.01 to 100%mol/mol) and diameters were monitored by Quartz Crystal Microbalance with Dissipation (QCM-D). The dissipation change per frequency change, i.e. acoustic ratio, which is evaluated as a degree of the viscoelasticity, considerably decreased with the presence of DPPTE (from 162.3GHz-1 for flattened PC liposomes to ca. 89.5GHz-1 for 100% DPPTE liposomes) when compared to the results of two reference rigid monolayers and two viscoelastic layers. To assess the quality of SLB platform, the interpretation of QCM-D data was also complemented with Surface Plasmon Resonance. The optimum thiolated-lipid ratio (1%, lower thiol ratio and higher rigidity) was then used to determine the dry-lipid mass deposition, the water content and the thickness values of the SLB via viscoelastic modelling. Further surface characterization studies were performed by Atomic Force Microscopy with high spatial resolution. The results suggested that model membrane was almost continuous with minimum defects but showed more dissipative/soft nature compared to an ideal bilayer due to partially fused liposomes/overlapped lipid bilayers/multilayer islands. These local elevations distorted the planarity and led the increase of overall membrane thickness to ∼7.0nm.


Assuntos
Ouro/química , Bicamadas Lipídicas/química , Fosfolipídeos/química , Compostos de Sulfidrila/química , Elasticidade , Bicamadas Lipídicas/metabolismo , Lipossomos/química , Lipossomos/metabolismo , Lipídeos de Membrana/química , Lipídeos de Membrana/metabolismo , Microscopia de Força Atômica , Fosfatidilcolinas/química , Fosfatidilcolinas/metabolismo , Fosfolipídeos/metabolismo , Técnicas de Microbalança de Cristal de Quartzo , Compostos de Sulfidrila/metabolismo , Ressonância de Plasmônio de Superfície , Propriedades de Superfície , Triglicerídeos/química , Triglicerídeos/metabolismo , Viscosidade
16.
IEEE Trans Nanobioscience ; 14(6): 572-80, 2015 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-26151941

RESUMO

Many neurodegenerative diseases arise from the malfunctioning neurons in the pathway where the signal is carried. In this paper, we propose neuron specific TDMA/multiplexing and demultiplexing mechanisms to convey the spikes of a receptor neuron over a neighboring path in case of an irreversible path fault existing in its original path. The multiplexing mechanism depends on neural delay box (NDB) which is composed of a relay unit and a buffering unit. The relay unit can be realized as a nanoelectronic device. The buffering unit can be implemented either via neural delay lines as employed in optical switching systems or via nanoelectronic delay lines, i.e., delay flip flops. Demultiplexing is realized by a demultiplexer unit according to the time slot assignment information. Besides, we propose the use of neural interfaces in the NDBs and the demultiplexer unit for detecting and stimulating the generation of spikes. The objective of the proposed mechanisms is to substitute a malfunctioning path, increase the number of spikes delivered and correctly deliver the spikes to the intended part of the somatosensory cortex. The results demonstrate that significant performance improvement on the successively delivered number of spikes is achievable when delay lines are employed as neural buffers in NDBs.


Assuntos
Modelos Neurológicos , Nanotecnologia/métodos , Redes Neurais de Computação , Neurônios/fisiologia , Potenciais de Ação/fisiologia , Eletrônica , Humanos
17.
N Biotechnol ; 32(6): 747-55, 2015 Dec 25.
Artigo em Inglês | MEDLINE | ID: mdl-25556119

RESUMO

TiN and (Ti,Mg)N thin film coatings were deposited on titanium substrates by using cathodic arc physical vapor deposition (arc-PVD) technique with magnesium contents of 0, 4.24 at% (low Mg) and 10.42 at% (high Mg). The presence of magnesium on both normal (hFOB) and cancer (SaOS-2) osteoblast cell behavior was investigated in (Ti,Mg)N surfaces with or without prior hydroxyapatite (HA) deposition (in simulated body fluid, SBF). Mg incorporation on TiN films was found to have no apparent effect on the cell proliferation in bare surfaces but cell spreading was better on low Mg content surface for hFOB cells. SaOS-2 cells, on the other hand, showed an increased extra cellular matrix (ECM) deposition on low Mg surfaces but ECM deposition almost disappeared when Mg content was increased above 10 at%. HA deposited surfaces with high Mg content was shown to cause a significant decrease in cell viability. While the cells were flattened, elongated and spread over the surface in contact with each other via cellular extensions on unmodified and low Mg doped surfaces, unhealthy morphologies of cells with round shape with a limited number of extended arms was visualized on high Mg containing samples. In summary, Mg incorporation into the TiN coatings by arc-PVD technique and successive HA deposition led to promising cell responses on low Mg content surfaces for a better osteointegration performance.


Assuntos
Materiais Revestidos Biocompatíveis/síntese química , Durapatita/química , Magnésio/química , Nitrogênio/química , Osteoblastos/fisiologia , Titânio/química , Ligas/química , Substitutos Ósseos/química , Adesão Celular/fisiologia , Linhagem Celular , Proliferação de Células/fisiologia , Matriz Extracelular/metabolismo , Humanos , Teste de Materiais , Osteoblastos/citologia
18.
Biochem Biophys Rep ; 2: 115-122, 2015 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-29124152

RESUMO

To investigate drug-membrane protein interactions, an artificial tethered lipid bilayer system was constructed for the functional integration of membrane proteins with large extra-membrane domains such as multi-drug resistance protein 1 (MDR1). In this study, a modified lipid (i.e., 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino (polyethylene glycol)-2000] (DSPE-PEG)) was utilized as a spacer molecule to elevate lipid membrane from the sensor surface and generate a reservoir underneath. Concentration of DSPE-PEG molecule significantly affected the liposome binding/spreading and lipid bilayer formation, and 0.03 mg/mL of DSPE-PEG provided optimum conditions for membrane protein integration. Further, the incorporation of MDR1 increased the local rigidity on the platform. Antibody binding studies showed the functional integration of MDR1 protein into lipid bilayer platform. The platform allowed to follow MDR!-statin-based drug interactions in vitro. Each binding event and lipid bilayer formation was monitored in real-time using Surface Plasmon Resonance and Quartz Crystal Microbalance-Dissipation systems, and Atomic Force Microscopy was used for visualization experiments.

19.
Mater Sci Eng C Mater Biol Appl ; 33(7): 4337-42, 2013 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-23910351

RESUMO

In this study, formation of magnesium substituted hydroxyapatite (Ca10-xMgx(PO4)6(OH)2) on (Ti,Mg)N and TiN coating surfaces were investigated. The (Ti1-x,Mgx)N (x=0.064) coatings were deposited on titanium substrates by using cathodic arc physical vapor deposition technique. TiN coated grade 2 titanium substrates were used as reference to understand the role of magnesium on hydroxyapatite (HA) formation. The HA formation experiments was carried out in simulated body fluids (SBF) with three different concentrations (1X SBF, 5X SBF and 5X SBF without magnesium ions) at 37 °C. The coatings and hydroxyapatite films formed were characterized by scanning electron microscope (SEM), X-ray diffraction (XRD) and FTIR Spectroscopy techniques. The energy dispersive X-ray spectroscopy (EDS) analyses and XRD investigations of the coatings indicated that magnesium was incorporated in the TiN structure rather than forming a separate phase. The comparison between the TiN and (Ti, Mg)N coatings showed that the presence of magnesium in TiN structure facilitated magnesium substituted HA formation on the surface. The (Ti,Mg)N coatings can potentially be used to accelerate the HA formation in vivo conditions without any prior hydroxyapatite coating procedure.


Assuntos
Materiais Revestidos Biocompatíveis/química , Durapatita/química , Compostos de Magnésio/química , Magnésio/química , Teste de Materiais/métodos , Compostos de Nitrogênio/química , Titânio/química , Líquidos Corporais/química , Eletrodos , Humanos , Microscopia Eletrônica de Varredura , Espectroscopia de Infravermelho com Transformada de Fourier , Difração de Raios X
20.
J Tissue Eng Regen Med ; 7(2): 149-60, 2013 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-22081628

RESUMO

The effects of double release of insulin-like growth factor I (IGF-I) and growth factor ß1 (TGF-ß1) from nanoparticles on the growth of bone marrow mesenchymal stem cells and their differentiation into cartilage cells were studied on PLGA scaffolds. The release was achieved by using nanoparticles of poly(lactic acid-co-glycolic acid) (PLGA) and poly(N-isopropylacrylamide) (PNIPAM) carrying IGF-I and TGF-ß1, respectively. On tissue culture polystyrene (TCPS), TGF-ß1 released from PNIPAM nanoparticles was found to have a significant effect on proliferation, while IGF-I encouraged differentiation, as shown by collagen type II deposition. The study was then conducted on macroporous (pore size 200-400 µm) PLGA scaffolds. It was observed that the combination of IGF-I and TGF-ß1 yielded better results in terms of collagen type II and aggrecan expression than GF-free and single GF-containing applications. It thus appears that gradual release of a combination of growth factors from nanoparticles could make a significant contribution to the quality of the engineered cartilage tissue.


Assuntos
Cartilagem/efeitos dos fármacos , Fator de Crescimento Insulin-Like I/farmacologia , Engenharia Tecidual/métodos , Fator de Crescimento Transformador beta1/farmacologia , Acrilamidas/farmacologia , Resinas Acrílicas , Agrecanas/metabolismo , Animais , Bovinos , Proliferação de Células/efeitos dos fármacos , Colágeno/metabolismo , Colágeno Tipo II/metabolismo , Matriz Extracelular/efeitos dos fármacos , Matriz Extracelular/metabolismo , Glicosaminoglicanos/metabolismo , Ácido Láctico/farmacologia , Masculino , Microscopia Confocal , Nanopartículas/ultraestrutura , Tamanho da Partícula , Ácido Poliglicólico/farmacologia , Copolímero de Ácido Poliláctico e Ácido Poliglicólico , Polímeros/farmacologia , Ratos , Ratos Sprague-Dawley , Reação em Cadeia da Polimerase em Tempo Real , Soroalbumina Bovina/metabolismo , Alicerces Teciduais/química
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