1.
Mol Biochem Parasitol
; 76(1-2): 325-8, 1996.
Artigo
em Inglês
| MEDLINE
| ID: mdl-8920021
2.
Anal Biochem
; 133(1): 197-207, 1983 Aug.
Artigo
em Inglês
| MEDLINE
| ID: mdl-6638480
RESUMO
A version of the methylation analysis of complex carbohydrates by gas chromatography-mass spectrometry of the methylalditol acetates (H. Björndal, C. G. Hellerquist, B. Lindberg, and S. Svensson (1970) Angew. Chem. 82, 643-674) is described. With this version 100- to 500-pmol samples of N-glycosidically linked glycoprotein oligosaccharides may be analyzed. The method is based on the use of capillary columns which allow the separation of all partially methylated alditol acetates potentially obtained from this group of oligosaccharides and on their selective and sensitive detection by mass fragmentography after chemical ionization with ammonia.
Assuntos
Glicoproteínas/análise , Oligossacarídeos/análise , Cromatografia Gasosa/métodos , Cromatografia Gasosa-Espectrometria de Massas/métodos , Hidrólise , Metilação
3.
Anal Biochem
; 121(2): 263-74, 1982 Apr.
Artigo
em Inglês
| MEDLINE
| ID: mdl-7103056