RESUMO
The number, size and shape of polymorphic plant mitochondria are determined at least partially by mitochondrial fission. Arabidopsis mitochondria divide through the actions of a dynamin-related protein, DRP3A. Another plant-specific factor, ELM1, was previously shown to localize DRP3A to mitochondrial fission sites. Here, we report that mitochondrial fission is not completely blocked in the Arabidopsis elm1 mutant and that it is strongly manifested in response to cold treatment. Arabidopsis has an ELM1 paralogue (ELM2) that seems to have only a limited role in mitochondrial fission in the elm1 mutant. Interestingly, cold-induced mitochondrial fragmentation was also observed in the wild-type, but not in a drp3a mutant, suggesting that cold-induced transient mitochondrial fragmentation requires DRP3A but not ELM1 or ELM2. DRP3A: GFP localized from the cytosol to mitochondrial fission sites without ELM1 after cold treatment. Together, these results suggest that Arabidopsis has a novel, cold-induced type of mitochondrial fission in which DRP3A localizes to mitochondrial fission sites without the involvement of ELM1 or ELM2.
Assuntos
Proteínas de Arabidopsis/metabolismo , Arabidopsis/fisiologia , Temperatura Baixa , Mitocôndrias/metabolismo , Sequência de Aminoácidos , Proteínas de Arabidopsis/química , Proteínas de Arabidopsis/genética , Mitocôndrias/genética , Dinâmica Mitocondrial , Mutação , FenótipoRESUMO
We used molecular simulation to study the static behavior of polymer droplets in vacuum and on solid surfaces, namely the size of the droplet and the contact angle, respectively. The effects of the polymer chain length and the total number of particles were calculated by the many-body dissipative particle dynamics method. For the spherical droplet containing the same number of particles, we show that its radius depends on the polymer chain length. The radius of the droplet is also proportional to one-third power of the total number of particles for all given chain lengths. For the hemispherical droplet, the contact angle increases with the number of particles in the droplet, and this effect is relatively strong, especially for longer polymer chains. The effect of wettability of the solid surface was also investigated by using polymerphobic (low-affinity) and polymerphilic (high-affinity) surfaces. As the chain length increases, the contact angle on the low-affinity surface decreases, while that on the hydrophilic surface increases. The simulation reveals that there is a critical affinity for the monomer on the solid surface; above and below which the wettability increases and decreases as the molecular length increases, respectively.
RESUMO
Mitochondrial fission is achieved partially by the activity of self-assembling dynamin-related proteins (DRPs) in diverse organisms. Mitochondrial fission in Arabidopsis thaliana is mediated by DRP3A and DRP3B, but the other genes and molecular mechanisms involved have yet to be elucidated. To identify these genes, we screened and analyzed Arabidopsis mutants with longer and fewer mitochondria than those of the wild type. ELM1 was found to be responsible for the phenotype of elongated mitochondria. This phenotype was also observed in drp3a plants. EST and genomic sequences similar to ELM1 were found in seed plants but not in other eukaryotes. ELM1:green fluorescent protein (GFP) was found to surround mitochondria, and ELM1 interacts with both DPR3A and DRP3B. In the elm1 mutant, DRP3A:GFP was observed in the cytosol, whereas in wild-type Arabidopsis, DRP3A:GFP localized to the ends and constricted sites of mitochondria. These results collectively suggest that mitochondrial fission in Arabidopsis is mediated by the plant-specific factor ELM1, which is required for the relocalization of DRP3A (and possibly also DRP3B) from the cytosol to mitochondrial fission sites.