RESUMO
Background: Gingivitis in response to biofilm formation may exhibit different trajectories. The purposes of the present study were to characterize the composition of the supragingival microbiota and salivary cytokine and protein levels in healthy individuals with different gingivitis patterns, to test the hypothesis that manifestations of gingivitis associate with specific profiles in terms of supragingival microbiota, salivary cytokines, and proteins. Methods: Forty orally and systemically healthy individuals refrained from all oral hygiene procedures for a period of 14 days, followed by a resolution period of 14 days with regular oral care. Supragingival plaque level and bleeding on probing (BOP) were recorded, and supragingival plaque as well as saliva samples were collected at baseline, day 14, and day 28. Based on change in BOP% from baseline to day 14, rapid (n = 15), moderate (n = 10), and slow (n = 15) responders were identified. Supragingival microbiota composition, salivary cytokine, and protein levels were compared between groups at baseline, day 14, and day 28. Results: A significantly higher baseline abundance of Capnocytophaga, Eikenella, and Campylobacter species were recorded in rapid responders, whereas a significantly higher baseline abundance of Streptococcus species were detected in slow responders. Slow responders expressed a high degree of resilience, with minimal difference in microbial composition at baseline and after 14 days of resolution (day 28). On the contrary, significant differences in relative abundance of members of the core microbiota, Streptococcus, Actinomyces, and Rothia species, was noted in baseline samples versus day 28 samples in rapid responders. Comparable baseline cytokine and protein levels were recorded in all groups. Conclusion: Supragingival microbiota composition, but not saliva cytokine and protein profiles, seems to influence the extent of the inflammatory response during development of gingivitis in systemically healthy individuals.
Baseline composition of the supragingival microbiota might predict different gingivitis trajectories.Microbial resilience after gingivitis might augment oral homeostasis in individuals with a slow gingivitis trajectory.
RESUMO
No disponible
Objectives: Treatment of dental discolorations with external bleaching is becoming very common in dentistry, however, possible irreversible alterations on enamel surface due to bleaching procedures is a topic of discussion. The aim of this study was to evaluate the clinical effects of bleaching by measuring the dental plaque accumulation on human enamel and tooth discoloration in vivo. Study Design: Forty-four teeth in eleven patients not revealing any restorations or periodontal problems were enrolled in this study. Bleaching agent applied only to labial surfaces of incisors using commercial 35 % hydrogen peroxide gel. Dental plaque and tooth color measured in the same group of participants, at the end of non-brushing periods lasting 3 and 5 days, respectively, before and after bleaching.Results: The results of the comparison of pre- and post-bleaching measurements showed that, after a non-brushingperiod lasting 3 day, discoloration scores and plaque accumulation scores for bleached surfaces were lower than the non-bleached surface scores. However, at the end of a non-brushing period lasting 5 day, even the color measurement score in post-bleaching period was lower than the pre-bleaching counterpart, plaque index measurements showed higher plaque accumulation scores in the bleached group.Conclusions: According to these results, bleaching with 35 % hydrogen peroxide seem to favor plaque accumulation after non-brushing period lasting 5 day and tooth discoloration after bleaching is not in correlation with the amount of plaque accumulation
