RESUMO
The "Thrombin Reference Standard (Control 031", of National Institute of Health Sciences was prepared. The precision of filling into ampoule was about 11.5% as C.V. The content of alpha-thrombin was about 89%. The thrombin potency of the standard material was assayed against the Thrombin Reference Standard (Control 961) according to the method of JP-X IV and the potency was 692 +/- 35 units/ampoule. From the results, the potency of the proposed material for Thrombin Reference Standard was defined as 690 units per ampoule.
Assuntos
Órgãos Governamentais , Trombina/normas , Eletroforese em Gel de Poliacrilamida , Japão , Farmacopeias como Assunto/normas , Padrões de Referência , Trombina/análiseRESUMO
The "Lysozyme Reference Standard (Control 951031)" of the National Institute of Health Sciences was prepared. The lysozyme potency of the standard material was assayed against the Lysozyme Reference Standard (Control 951) by turbidimetric method two turbidimetric methods using the dried y-cells of Micrococcus luteus as a substrate. The potency of the standard material was in satisfactory agreement with that of Lysozyme Reference Standard (Control 951) and was defined as 1 mg [potency] per mg.
Assuntos
Órgãos Governamentais , Muramidase/normas , Aminoácidos/análise , Eletroforese em Gel de Poliacrilamida , Japão , Micrococcus luteus/citologia , Muramidase/análise , Muramidase/isolamento & purificação , Nefelometria e Turbidimetria/métodos , Farmacopeias como Assunto/normas , Padrões de ReferênciaRESUMO
Plasticizers in cap-sealing for bottled foods were analyzed. Twenty-three domestic samples and 80 imported samples, a total of 103 samples, were tested. Among them, 93 contained chloride, and 62 contained di(2-ethylhexyl) phthalate (DEHP), diisodecyl phthalate, O-acetyl tributyl citrate, diacetyllauroyl glycerol, di(2-ethylhexyl) adipate, diisononyl phthalate or dicyclohexyl phthalate. Twelve samples with DEHP-containing caps were further tested for plasticizer levels in the foods. Higher DEHP was detected in oily and fluid food specimens. However, the intake of plasticizers calculated based on usual food consumption did not exceed the tolerable daily intake level in any of the cases. A food sample containing the highest level of DEHP was stored under various conditions, and DEHP that migrated from the cap-sealing into the food was determined. Shaking the bottles increased migration of DEHP into foods.
