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BACKGROUND:Osteonecrosis of the femoral head is a common orthopedic disease,and hip preservation surgery with bone grafting is commonly used in the early stage,in which autologous bone and allograft bone are commonly used as bone grafting materials.However,autologous bone transplantation is highly traumatic and bone supply is limited,and allograft bone is rich in sources,but there are serious risks of immune rejection and absorption.In recent years,the tissue engineering technique based on mesenchymal stem cells is a new method for the treatment of femoral head necrosis,which is gradually widely used after basic experiments and clinical application. OBJECTIVE:To review the application and prospect of tissue engineering in the treatment of osteonecrosis of the femoral head to provide a new choice for the clinical treatment of osteonecrosis of the femoral head. METHODS:The PubMed database and CNKI database from 2013 to 2023 were searched by the first author with Chinese and English search terms"tissue engineering,mesenchymal stem cells,biological scaffolds,cytokines,osteonecrosis of the femoral head,bone graft,hip preservation".The articles on the treatment of osteonecrosis of the femoral head with tissue engineering technology were selected,and 55 representative articles were included for review after the initial screening of all articles according to the inclusion and exclusion criteria. RESULTS AND CONCLUSION:(1)With the continuous development of biotechnology and materials science,great progress has been made in the treatment of osteonecrosis of the femoral head by bone tissue engineering,such as the application of gene-modified mesenchymal stem cells to repair osteonecrosis,the combination of gene recombination technology and surface modification technology with bone tissue engineering in the treatment of osteonecrosis of the femoral head.(2)When applied to the necrotic femoral head,tissue engineering technology can promote the regeneration of necrotic bone tissue and the repair of the vascular system,provide biomechanical stability for the necrotic area,and use bioactive factors to accelerate the repair of seed cells to complete the regeneration of new bone in necrotic area.(3)However,most of these studies are still in the animal experiment stage,and there are still many unsolved problems and challenges in bone tissue engineering research.With the rapid development of nanotechnology,tissue engineering and clinical medicine,biomimetic replacement bone grafting materials with perfect performance are expected to come into being.(4)In the future,bone tissue engineering for osteonecrosis of the femoral head is expected to be a satisfactory treatment for patients with hip preservation.
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Objective To investigate the clinical effects and the degree of satisfaction of areola margin small incision and conventional incision in the treatment of patients with benign tumor of mammary glands. Methods 338 patients were selected and randomly divided into the observation group and the control group according to the digital table,169 patients in each group. The clinical effects and the degree of satisfaction of the patients were compared. Results Compared with the control group, the operation time was slightly longer in the observation group[(26. 2 ± 4. 8) min vs. (24. 5 ± 3. 7) min,t =13. 178,P =0. 000];The incidence rate of postoperative complications was significantly reduced (2. 37% vs. 34. 55%,χ2 =57. 911,P=0. 000);The scar score wassignificantlylower[(1.11±0.47)pointsvs.(4.14±0.45)points,t= -60.387,P=0.000];Thepostoperative satisfaction rate was significantly higher (98. 82% vs. 62. 13%,χ2 =72. 379,P =0. 000). Conclusion Areola margin small incision has high degree of satisfaction and small scar,provides safe and reliable effect in treatment of benign tumor of mammary glands. This method can be suitable for popularization and application in clinic.
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Objective To investigate the clinical effects and the degree of satisfaction of areola margin small incision and conventional incision in the treatment of patients with benign tumor of mammary glands. Methods 338 patients were selected and randomly divided into the observation group and the control group according to the digital table,169 patients in each group. The clinical effects and the degree of satisfaction of the patients were compared. Results Compared with the control group, the operation time was slightly longer in the observation group[(26. 2 ± 4. 8) min vs. (24. 5 ± 3. 7) min,t =13. 178,P =0. 000];The incidence rate of postoperative complications was significantly reduced (2. 37% vs. 34. 55%,χ2 =57. 911,P=0. 000);The scar score wassignificantlylower[(1.11±0.47)pointsvs.(4.14±0.45)points,t= -60.387,P=0.000];Thepostoperative satisfaction rate was significantly higher (98. 82% vs. 62. 13%,χ2 =72. 379,P =0. 000). Conclusion Areola margin small incision has high degree of satisfaction and small scar,provides safe and reliable effect in treatment of benign tumor of mammary glands. This method can be suitable for popularization and application in clinic.
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Objective To observe the effect of serum containing Qiangjijianli oral liquid in-vitro proliferation of rat mesenchymal stem cells (MSCs). Methods Rat mesenchymal stem cells dissociated from the bone marrow by density gradient method were cultured. MSCs were identified by marking of bromodeoxyuridine (Brdu) and staining of CD44, CD45. Experimental group was cultivated with serum containing Qiangjijianli oral liquid and control group with blank serum. Optical absorption value of MSCs was stained by methyl thiazolyl tetrazolium (MTT). Results Serum containing Qiangjijianli oral liquid at different concentrations could promote the proliferation of MSCs, the difference being significant in comparison with the control group (P
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0.05),but 5-Aza at the concentration of 15?mol/L inhibited the growth of MSCs(P
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[Objective] To explore the relationship between TCM syndrome patterns and physicochemical parameters of chronic obstructive pulmonary disease (COPD). [Methods] Five hundred hospitalized cases of COPD were investigated. The correlation of TCM syndrome patterns with pulmonary function, blood analysis and biochemical indexes were assayed by logistic forward regression analysis and linear multiple regression analysis, and then the above indexes were analyzed by independent- samples t test. [Results] Five equations were obtained by logistic regression. Positive correlation was found between the number of white blood cells (WBC) and the pattern of phlegm-heat blocking lung, hemoglobin (HGB) level and the pattern of yang deficiency with fluid retention, as well as red cell distribution width (RDW) and the pattern of kidney deficiency. Maximal expiratory flow rate in residual 75 % vital capacity ( MEF75), peak of expiratory flow (PEF) and mean corpuscular hemoglobin (MCH) were negatively correlated with the pattern of yang deficiency with fluid retention. Maximal expiratory flow rate in residual 25% vital capacity (MEF25) and granulocyte percentage (GP) were also negatively correlated with the pattern of spleen deficiency. So did potassium ions (K+ ) level and the pattern of kidney deficiency, t test showed the same results. [Conclusion] The above indexes have great value for TCM syndrome differentiation of COPD.