Differences in predator-avoidance behavior between two invasive gobies and their native competitors.

Globally, fish are frequently introduced beyond their native range. Some, like Ponto-Caspian gobies, are becoming invasive, achieving high colonization rates and constituting frequent prey for native predators. However, little is known about the effectiveness of antipredator behaviors of the invaders, which may shape their role in the invaded community and contribute to the invasion success. We compared antipredator behaviors of invasive gobies and native fish species after their detection by the predator, when the danger becomes direct. We studied 2 fish pairs, each consisting of an invasive and native species co-occurring in the environment and belonging to the same prey guild: (1) the racer goby Babka gymnotrachelus versus European bullhead Cottus gobio, (2) the monkey goby Neogobius fluviatilis versus gudgeon Gobio gobio, facing a naïve predator (the Eurasian perch Perca fluviatilis). We analyzed behaviors of single prey individuals (escaping, staying in shelter, and activity) and single predators (activity, searching, following, capturing, and latency to prey consumption). In the predator presence, the bullhead was less active and more often managed to escape after capture than the racer goby. The gudgeon escaped before the capture more often than the monkey goby. The predator succeeded later with the bullhead compared to racer goby, whereas no differences in ingestion time occurred between the gudgeon and monkey goby. The results suggest that, in terms of hunting effort of native predators, the invasive gobies are equivalent to or more profitable prey than their native analogs, which can facilitate the integration of the gobies into local food webs.

Disruptive effect of artificial light at night on leaf litter consumption, growth and activity of freshwater shredders.

Artificial light at night (ALAN) is a globally widespread phenomenon potentially affecting ecosystem processes, such as leaf litter breakdown, which is a source of organic matter in fresh waters. Here, we conducted a long-term experiment to test the effects of ALAN (2 lx) differing in spectral composition: white LEDs and high pressure sodium lamps (HPS) on leaf consumption, growth and activity of two macroinvertebrate species of shredders: Gammarus jazdzewskii and Dikerogammarus villosus (Crustacea, Amphipoda), compared to the undisturbed light-dark cycle. We also tested if the nocturnal illumination would influence the algal community colonising leaves, which is an important component of the leaf-shredder diet. We found that LED light increased the consumption of leaves by both species, which was nearly twice as high as in other treatments, and supressed the growth rate of G. jazdzewskii, whereas the growth of D. villosus was not affected by either light type. Moreover, D. villosus reduced its activity when exposed to ALAN of both types. As ALAN-induced changes in shredder growth and consumption were not associated with their increased activity or decreased food quality, we suggest that LED light may be a source of physiological stress for shredders, raising their energy expenditure, which was compensated by increased food intake. We have shown that LED illumination induces greater effects on wildlife than alternative, narrow wavelength spectrum light sources, such as HPS lamps, and may potentially alter the litter breakdown in aquatic ecosystems. It may accelerate the turnover of leaves by shredders, but on the other hand, it may negatively affect the fitness of macroinvertebrates and thus disturb the leaf processing over a longer term.

All Shades of Shrimp: Preferences of Colour Morphs of a Freshwater Shrimp Neocaridina davidi (Decapoda, Atyidae) for Substrata of Different Colouration.

An ornamental freshwater shrimp, Neocaridina davidi, is popular as an aquarium hobby and, therefore, a potentially invasive species. There is a growing need for proper management of this species to determine not only their optimum breeding conditions, but also their ability to colonise novel environments. We tested habitat preferences of colour morphs (brown, red, white) of N. davidi for substratum colour (black, white, grey shades, red) and fine or coarse chess-board patterns to recognise their suitable captivity conditions and predict their distribution after potential release into nature. We conducted laboratory choice experiments (n = 8) with three individuals of the same morph exposed for two hours to a range of backgrounds. Shrimp preferred dark backgrounds over light ones irrespective of their own colouration and its match with the background colour. Moreover, the brown and red morphs, in contrast to the white morph, preferred the coarse background pattern over the finer pattern. This suggests that the presence of dark, uniform substrata (e.g., rocks, macrophytes) will favour N. davidi. Nevertheless, the polymorphism of the species has little effect on its total niche breadth, and thus its invasive potential.

Combined effects of nocturnal exposure to artificial light and habitat complexity on fish foraging.

Due to the widespread use of artificial light, freshwater ecosystems in urban areas at night are often subjected to light of intensities exceeding that of the moonlight. Nocturnal dim light could modify fish behaviour and benefit visual predators because of enhanced foraging success compared to dark nights. However, effects of nocturnal light could be mitigated by the presence of structured habitats providing refuges for prey. We tested in laboratory experiments whether nocturnal light of low intensity (2 lx) increases foraging efficiency of the Eurasian perch (Perca fluviatilis) on invertebrate prey (Gammarus fossarum). The tests were conducted at dusk and night under two light regimes: natural cycle with dark nights and disturbed cycle with artificially illuminated nights, in habitats differing in structural complexity: sand and woody debris. We found that nocturnal illumination significantly enhanced the consumption of gammarids by fish compared to dark nights. In addition, the perch was as effective predator in illuminated nights (2 lx) as at dusk (10 lx). Woody debris provided an effective refuge only in combination with undisturbed darkness, but not in illuminated nights. Our results suggest that nocturnal illumination in aquatic ecosystems may contribute to significant reductions in invertebrate population sizes through fish predation. The loss of darkness reduces the possibility of using shelters by invertebrates and hence the effects of elevated light levels at night could not be mitigated by an increased habitat complexity.

Zebra mussel beds: an effective feeding ground for Ponto-Caspian gobies or suitable shelter for their prey?

Aggregations of the Ponto-Caspian invasive zebra mussel (Dreissena polymorpha) constitute a suitable habitat for macroinvertebrates, considerably increasing their abundance and providing effective antipredator protection. Thus, the overall effect of a mussel bed on particular predator species may vary from positive to negative, depending on both prey density increase and predator ability to prey in a structurally complex habitat. Alien Ponto-Caspian goby fish are likely to be facilitated when introduced into new areas by zebra mussels, provided that they are capable of utilizing mussel beds as habitat and feeding grounds. We ran laboratory experiments to find which prey (chironomid larvae) densities (from ca. 500 to 2,000 individuals m-2) in a mussel bed make it a more beneficial feeding ground for the racer goby Babka gymnotrachelus (RG) and western tubenose goby Proterorhinus semilunaris (WTG) compared to sandy and stone substrata (containing the basic prey density of 500 ind. m-2). Moreover, we checked how food availability affects habitat selection by fish. Mussel beds became more suitable for fish than alternative mineral substrata when food abundance was at least two times higher (1,000 vs. 500 ind. m-2), regardless of fish size and species. WTG was associated with mussel beds regardless of its size and prey density, whereas RG switched to this habitat when it became a better feeding ground than alternative substrata. Larger RG exhibited a stronger affinity for mussels than small individuals. WTG fed more efficiently from a mussel bed at high food abundances than RG. A literature review has shown that increasing chironomid density, which in our study was sufficient to make a mussel habitat an attractive feeding ground for the gobies, is commonly observed in mussel beds in the field. Therefore, we conclude that zebra mussels may positively affect the alien goby species and are likely to facilitate their establishment in novel areas, contributing to an invasional meltdown in the Ponto-Caspian invasive community.

Histological, histochemical and ultrastructural analysis reveals functional division of the oesophagogastric segment in freshwater tubenose goby Proterorhinus semilunaris Heckel, 1837.

Histological and histochemical features of the oesophagogastric segment of the alimentary canal as well as ultrastructure of gastric gland cells of freshwater tubenose goby Proterorhinus semilunaris were examined. The studies revealed that despite the lack of anatomical distinction, the oesophagogastric segment is histologically divided into the oesophagus, oesogaster and stomach, which provides evidence for the functional compartmentation of this organ. The oesophagus was characterised by the presence of numerous goblet cells secreting mainly a mixture of neutral and acid mucopolysaccharides. In the stomach, the apical zone of the surface epithelial cells contained neutral mucopolysaccharides. Numerous proliferating cells were scattered throughout the surface epithelium. In the lamina propria of the stomach, a well-developed layer of gastric glands was observed. The glands were of the alveolar type and occupied nearly the entire length of the stomach except the pyloric region. The gastric gland cells were varied into light and dark; however, their ultrastructure was identical. All cells had numerous mitochondria and a well-developed tubulovesicular system typical for the oxynticopeptic cells, but pepsinogen granules were not present in the cytoplasm of these cells. These findings contribute new evidence to literature reports that not all gobiid fish are stomachless. Moreover, they suggest higher adaptation of the species to utilise protein-rich food compared to stomachless fish, and its ability to adjust the alimentary canal quickly to changing diet. How this may facilitate establishment of P. semilunaris in invaded environments remains an open question.

Gyrodactylus proterorhini Ergens, 1967 (Monogenoidea, Gyrodactylidae) in gobiids from the Vistula River--the first record of the parasite in Poland.

During a parasitological survey of two non-native fish species-racer goby Neogobius gymnotrachelus and monkey goby Neogobius fluviatilis-in the Wloclawek Reservoir on the lower Vistula River, the monogenean Gyrodactylus proterorhini was recorded for the first time in Poland, and for the first time, the racer goby was listed as the parasite host. Specimens of G. proterorhini were detected on body surface, fins, and gills of the fish studied. In 2006, the monkey goby was infected with the prevalence 41%, racer goby with the prevalence of 47%, at maximal intensity of 6 and 9 parasites per fish, (respectively). The infection level in relation to the fish size and sampling season was also discussed. The presented study supports the hypothesis of progressive introduction of the parasite with gobiids to the colonized areas.

First record of Nippotaenia mogurndae Yamaguti and Miyata, 1940 (Cestoda, Nippotaeniidae), a parasite introduced with Chinese sleeper to Poland.

A parasitological survey of an Asian fish, Chinese sleeper Perccottus glenii, inhabiting the Wloclawek Reservoir on the lower Vistula River (central Poland) revealed the occurrence of an exotic cestode Nippotaenia mogurndae Yamaguti and Miyata, 1940, which spreads spontaneously with a host to European waters. This paper documented the first record of the parasite in Poland. Specimens were described, and the effect of fish size and season on the infection level was analyzed. Within the 3 years, 112 fish were examined. Samples were collected by electrofishing in the upper part of the Wloclawek Reservoir three times (seasonally) in 2006 and 2007 and once in autumn 2008. Parasitological indices (prevalence, intensity of infection, and abundance) in particular samples as well as in two size groups of fish were calculated. Statistical tests were applied to analyze the results (Spearman's correlation coefficient and Mann-Whitney's U test). The infection level of Chinese sleeper with N. mogurndae in the Wloclawek Reservoir (prevalence 54.7%, mean intensity of infection 7.2, maximum 29) was comparable to infection noted in the area of the natural range of the host. The infection of smaller fish (less than 80 mm, TL) increased gradually with increasing body size. The infection of bigger specimens was in general higher, but the parasite recruitment was of a more incidental character. The spreading of N. mogurndae with Chinese sleeper is still in progress. The Wloclawek Reservoir provides suitable conditions for parasite development.