RESUMO
The main function of the Kis-Balaton Water Protection System is to retain nutrients and total suspended solids, thus protecting the water quality of Lake Balaton. In this paper, the toxic nature of the sediment in the 2nd reservoir of the KBWPS has been characterised, using a battery of tests: Vibrio fischeri acute bioassay on whole sediment samples, and V. fischeri bioassay on pore water and elutriate samples. The latest version of the V. fischeri bioluminescence inhibition was applied, the Flash assay which uses a kinetic mode and is able to detect the toxicity of solid, turbid/coloured samples. Whole sediment toxicity showed a clear spatial distribution of toxicity, in parallel with elutriate toxicity. However, no pore water toxicity was detected, leading to the conclusion that contaminants are not water soluble.
Assuntos
Sedimentos Geológicos/análise , Hidrocarbonetos Policíclicos Aromáticos/análise , Aliivibrio fischeri , Hungria , Lagos , Hidrocarbonetos Policíclicos Aromáticos/toxicidade , Qualidade da ÁguaRESUMO
Fluorochromes in combination with flow cytometry can be used for laboratory assessment of semen quality in humans and domestic animals. Some studies have reported the potential toxicity of these fluorochromes toward the cells analyzed, but not toward the laboratory technician who operates the analytical instrument. We tested the genotoxic potential of three fluorochromes, SYBR-14, propidium iodide, and Hoechst 33342, using the colorimetric SOS ChromoTest(™). The test revealed no genotoxic potential for any of the three fluorochromes within the dilution ranges investigated. We conclude that occasional direct contact with these fluorescent probes does not necessarily pose a genotoxic hazard.
