RESUMO
Whether a commercially available scanner might be used as a vertical photometer of 96-well microplates for enzyme immunoassay (EIA) was studied. The analytical characteristics of the scanner in this regard were assessed, by comparing the findings with the respective photometric data obtained by a "VICTOR" EIA analyzer. It is concluded that the scanner registration system in combination with "Expert-Lab EIA" may be used in all types of EIA. The scanner system provides additional possibilities as compared with the conventional photometric analyzers.
Assuntos
Técnicas Imunoenzimáticas/instrumentação , Microquímica/métodos , Processamento de Sinais Assistido por Computador/instrumentação , Biomarcadores/sangue , HumanosRESUMO
The present paper describes the use of the scanner "Expert-Lab Agglutination" system to record the results of latex-agglutination tests in making a reaction on the lids of 96-well immunoassay plates. The described system provides a possibility of having a primary image of all tests carried out on a carrier. Software offers the prospect of contrasting the images of individual and control samples, increasing the size of images, and comparing the latter. The use of special image treating techniques may also yield objective figures characterizing the rate of a reaction. The possibility of archiving primary information and retrospectively appraising the correctness of the result of an analysis at any required moment is of fundamental importance to the latex-agglutination test.