RESUMO
A comparative analysis was made of 3 conventional tests for tuberculosis (TB) versus a DNA probe technique among suspected TB patients at a reference centre in Greece. During 2004, we tested 2961 biological specimens from 2234 patients with the following methods: Ziehl-Neelsen staining, LöwensteinOJensen culture, BACTEC mycobacteria growth indicator tubes (MGIT) and the Gen-Probe AMPLIFIED Mycobacterium tuberculosis direct test (MTD). Of a total of 136 TB patients diagnosed and under anti-TB treatment, 133 of them (98%) were positive by amplified MTD. There were 112 TB (82%) detected by the MGIT method, 102 (75%) by Löwenstein-Jensen culture and 75 (55%) by Ziehl-Neelsen staining. Using MTD the positive result is ready within hours compared with days or weeks.
Assuntos
Técnicas Bacteriológicas/métodos , Sondas de DNA , Mycobacterium tuberculosis , Tuberculose/diagnóstico , Tuberculose/microbiologia , Técnicas Bacteriológicas/economia , Técnicas Bacteriológicas/normas , Análise Custo-Benefício , Meios de Cultura/economia , Sondas de DNA/economia , DNA Bacteriano/genética , Monitoramento de Medicamentos , Grécia , Humanos , Programas de Rastreamento , Mycobacterium tuberculosis/genética , Mycobacterium tuberculosis/crescimento & desenvolvimento , Técnicas de Amplificação de Ácido Nucleico/economia , Técnicas de Amplificação de Ácido Nucleico/métodos , Técnicas de Amplificação de Ácido Nucleico/normas , Sensibilidade e Especificidade , Fatores de Tempo , Tuberculose/tratamento farmacológicoRESUMO
A comparative analysis was made of 3 conventional tests for tuberculosis [TB] versus a DNA probe technique among suspected TB patients at a reference centre in Greece. During 2004, we tested 2961 biological specimens from 2234 patients with the following methods: Ziehl-Neelsen staining, LowensteinoJensen culture, BACTEC mycobacteria growth indicator tubes [MGIT] and the Gen-Probe AMPLIFIED[TM] Mycobacterium tuberculosis direct test [MTD]. Of a total of 136 TB patients diagnosed and under anti-TB treatment, 133 of them [98%] were positive by amplified MTD. There were 112 TB [82%] detected by the MGIT method, 102 [75%] by Lowenstein-Jensen culture and 75 [55%] by Ziehl-Neelsen staining. Using MTD the positive result is ready within hours compared with days or weeks
