[Application of bacterial thermo- and phagelysates for suppression of malignant tumor growth in experimental studies: 2 comparative analysis of anticancer efficacy of thermo- and phagelysates of Ps. aeruginosa and E. coli].

In the presented work antitumor effects of thermo- and phagelysates of Ps.aeruginosa and E.coli on Ehrlich carcinoma growth in mice have been studied. The treatment efficacy was evaluated according to the dynamic changes in volume of cancer tissue, cancer growth inhibition percent and calculations using Semi-empirical mathematical model describing cancer volume variations in relation to time passed after carcinoma inoculation. It was shown that at the early stage of cancer growth all tested bacterial preparations significantly inhibit cancer growth. Antitumor treatment effects were better expressed in animal studies using bacterial pagelysates in comparison to that of thermolysates. Comparative analysis of anticancer treatment efficacy of Ps.aeruginosa and E.coli phagelysates have shown that E.coli phagelysates reveal stronger and more prolonged anticancer treatment properties than phagelysates of Ps.aeruginosa.

[Application of bacterial thermo-and phagelysates for suppression of malignant tumor growth in experimental studies: 1. Anticancer efficacy of thermo- and phagelysates of E.coli].

Immunotherapy is considered as one of the promising treatment strategies for patients with malignant tumors. In the presented work antitumor effects of E.coli thermo- and phagelysates on Ehrlich carcinoma growth in mice have been studied. The treatment efficacy was evaluated by estimation of dynamic changes in volume of cancer tissue and by the percentage of cancer growth inhibition. Semi-empirical mathematical model, describing cancer volume variations in relation to time, passed after Ehrlich carcinoma inoculation was elaborated. It was shown that at the early stage of cancer growth both tested bacterial preparations significantly inhibit cancer growth. Antitumor treatment effects were better expressed in animal studies using bacterial phagelysates in comparison to that of thermolysates. After cessation of administration of bacterial preparations a prolonged antitumor activity in animals treated with E. coli phagelysates was demonstrated.

[New solution for cells and organs tissue conservation].

One of the major problems in organ and tissue transplantation is the conservation of donor's material from the moment of its impressments till its grafting in the recipient's organism. The new direction in transplantation is a method of liver tissue micro-fragments transplantation in bio-container Kakabadze, which was elaborated at the Department of Clinical Anatomy of Georgian State Medical Academy. In this work the results of investigation of hepatocytes' viability in micro-fragments were studied. The micro-fragments were placed in different medias, including the one elaborated by us on the bases of natural sugar-containing preservative. The decrease of percentage of viable hepatocytes in dependence of preservation time in different media was investigated. In all cases the dependence can be approximated by exponential function. On the bases of experimental data semi-empirical mathematical model of processes taking place in liver tissue micro-fragments during their preservation was created. Two parameters are introduced: one of them A depends on the method of micro-dissection, and the other T (1/2) is characteristic of the media and represents the time, in which the percentage of viable hepatocytes halves. The percentage of nonviable hepatocytes and also the percentage hepatocytes, which were in condition of apoptosis, were investigated. The investigations had shown that in all cases 50% barrier did not exceed 17 hours. This barrier in solution that is elaborated by us reached 46, 4 hours. Besides, if we assume, that the half of hepatocytes, which were in apoptosis condition, will become viable after transplantation, then the barrier will run up to 125 hours. The investigations of intra-cell glycogen showed that in all medias we had used, resources of glycogen were exhausted during one hour. In the case of solution elaborated by us glycogen was maintained in cells during 7-8 days.

[Organization of simple sequences in the Drosophilia melanoga ter genome]. / Organizatsiia prostykh posledovatel'nostei v genome Drosophilia melanogaster.

Fragments of Drosophila melanogaster DNA that intensively hybridize with simple sequences poly[(dG-dT).(dC-dA)], poly[(dA).(dT)] and poly[(dG-dA).(dC-dT)] were cloned. The first two types of simple sequences are organized in these clones as separated stretches of moderate length, repeated many times within 12-15 kb. Each cluster contains only one type of the simple sequences and originates from a unique in the genome. In contrast, poly[(dG-dA).(dC-dT)] occurs in the genome as several isolated motifs.

Nuclear proteins from Drosophila melanogaster embryos which specifically bind to simple homopolymeric sequences poly [(dT-dG).(dC-dA)].

The binding of nuclear proteins from Drosophila melanogaster embryos to simple homopolymeric DNA sequences was studied. Nuclear proteins were electrophoresed, transferred onto nitrocellulose and incubated with labelled synthetic homopolymers or natural fragment containing simple sequences. Several protein bands were found in the 65-72 KDa region, which specifically bind both poly [(dG-dT).(dA-dC)] and a natural fragment containing 40 bp of this sequence. These proteins do not bind to homopolymers poly [(dA).(dT)] and poly [(dG-dA).(dC-dT)], or other foreign DNAs.

[Nuclear proteins from Drosophila melanogaster specifically binding to simple homopolymeric sequences of poly[d(T-G)].poly[d(C-A)] type]. / Iadernye belki Drosophila melanogaster, spetsificheski sviazyvaiushchie prostye gomopolimernye posledovatel'nosti tipa poli[d(T-G)].poli[d(C-A)]

Binding of simple homopolymeric sequences to Drosophila melanogaster nuclear proteins has been studied. Proteins with Mr 65-72 kDa have been found, which specifically bind to synthetic poly[d(T-G)].poly[d(C-A)], as well as to D. melanogaster DNA containing a block of poly[d(T-G)].poly[d(C-A) 40 b.p. in length. It has been shown, that these proteins bind only to poly[d(T-G).poly[d(C-A)] and not to other types of simple sequences, for example poly[d(G-A)].poly[d(T-C)] and poly[d(A-T)].

Strand-specific transcription of the simple sequences poly[dG-dT):(dC-dA)] and poly[(dG-dA):(dC-dT)] in D. melanogaster embryos revealed by S1 nuclease treatment.

The transcription of different strands of the simple sequences poly[(dG-dT):(dC-dA)] and poly[(dG-dA):(dT-dC)] in Drosophila melanogaster embryos was studied. After selective labelling of one strand, the polymers were hybridized with immobilized RNA and hybrids were treated on filters with S1 nuclease. Appropriate control has shown that this treatment destroys sandwich-like complexes and leaves only true hybrids. Transcription of poly[(dG-dA):(dT-dC)] proved to be nearly symmetric, while poly(dG-dT) is transcribed 3-times more intensively than poly(dA-dC). Our approach allowed us to estimate the relative content of simple sequences in RNA.