A metabolic role for CD47 in pancreatic ß cell insulin secretion and islet transplant outcomes.

Diabetes is a global public health burden and is characterized clinically by relative or absolute insulin deficiency. Therapeutic agents that stimulate insulin secretion and improve insulin sensitivity are in high demand as treatment options. CD47 is a cell surface glycoprotein implicated in multiple cellular functions including recognition of self, angiogenesis, and nitric oxide signaling; however, its role in the regulation of insulin secretion remains unknown. Here, we demonstrate that CD47 receptor signaling inhibits insulin release from human as well as mouse pancreatic ß cells and that it can be pharmacologically exploited to boost insulin secretion in both models. CD47 depletion stimulated insulin granule exocytosis via activation of the Rho GTPase Cdc42 in ß cells and improved glucose clearance and insulin sensitivity in vivo. CD47 blockade enhanced syngeneic islet transplantation efficiency and expedited the return to euglycemia in streptozotocin-induced diabetic mice. Further, anti-CD47 antibody treatment delayed the onset of diabetes in nonobese diabetic (NOD) mice and protected them from overt diabetes. Our findings identify CD47 as a regulator of insulin secretion, and its manipulation in ß cells offers a therapeutic opportunity for diabetes and islet transplantation by correcting insulin deficiency.

Dry fruit image dataset for machine learning applications.

Dry fruits are convenient and nutritious snacks that can provide numerous health benefits. They are packed with vitamins, minerals, and fibres, which can help improve overall health, lower cholesterol levels, and reduce the risk of heart disease. Due to their health benefits, dry fruits are an essential part of a healthy diet. In addition to health advantage, dry fruits have high commercial worth. The value of the global dry fruit market is estimated to be USD 6.2 billion in 2021 and USD 7.7 billion by 2028. The appearance of dry fruits is utilized for assessing their quality to a great extent, requiring neat, appropriately tagged, and high-quality images. Hence, this dataset is a valuable resource for the classification and recognition of dry fruits. With over 11500+ high-quality processed images representing 12 distinct classes, this dataset is a comprehensive collection of different varieties of dry fruits. The four dry fruits included in this dataset are Almonds, Cashew Nuts, Raisins, and Dried Figs (Anjeer), along with three subtypes of each. This makes it a total of 12 distinct classes of dry fruits, each with its unique features, shape, and size. The dataset will be useful for building machine learning models that can classify and recognize different types of dry fruits under different conditions, and can also be beneficial for dry fruit research, education, and medicinal purposes. Due to their nutritional value and health advantages, dry fruits have been consumed for a very long time. One of the best strategies to improve general health is to include dry fruits in the diet.

No Time to Die-How Islets Meet Their Demise in Transplantation.

Islet transplantation represents an effective treatment for patients with type 1 diabetes mellitus (T1DM) and severe hypoglycaemia unawareness, capable of circumventing impaired counterregulatory pathways that no longer provide protection against low blood glucose levels. The additional beneficial effect of normalizing metabolic glycaemic control is the minimisation of further complications related to T1DM and insulin administration. However, patients require allogeneic islets from up to three donors, and the long-term insulin independence is inferior to that achieved with solid organ (whole pancreas) transplantation. This is likely due to the fragility of islets caused by the isolation process, innate immune responses following portal infusion, auto- and allo-immune-mediated destruction and ß-cell exhaustion following transplantation. This review covers the specific challenges related to islet vulnerability and dysfunction that affect long-term cell survival following transplantation.

Thrombospondin-1 CD47 Signalling: From Mechanisms to Medicine.

Recent advances provide evidence that the cellular signalling pathway comprising the ligand-receptor duo of thrombospondin-1 (TSP1) and CD47 is involved in mediating a range of diseases affecting renal, vascular, and metabolic function, as well as cancer. In several instances, research has barely progressed past pre-clinical animal models of disease and early phase 1 clinical trials, while for cancers, anti-CD47 therapy has emerged from phase 2 clinical trials in humans as a crucial adjuvant therapeutic agent. This has important implications for interventions that seek to capitalize on targeting this pathway in diseases where TSP1 and/or CD47 play a role. Despite substantial progress made in our understanding of this pathway in malignant and cardiovascular disease, knowledge and translational gaps remain regarding the role of this pathway in kidney and metabolic diseases, limiting identification of putative drug targets and development of effective treatments. This review considers recent advances reported in the field of TSP1-CD47 signalling, focusing on several aspects including enzymatic production, receptor function, interacting partners, localization of signalling, matrix-cellular and cell-to-cell cross talk. The potential impact that these newly described mechanisms have on health, with a particular focus on renal and metabolic disease, is also discussed.