Are pufferfishes a viable source of nutritional value for human consumption-an investigation on recently commercialized marine pufferfishes (Tetraodontidae: Tetraodontiformes).

Estimation of the nutritional profile of edible fishes is essential, and thus, we have evaluated nutritional composition and amino and fatty acids profiling of recently commercialized marine pufferfishes from the coast of Mandapam in the Gulf of Mannar. Proximate analyses including assessment of ash, moisture, proteins, carbohydrates, and lipids in the muscles of five edible marine pufferfishes were carried out. Major fatty acids profile of five fish species disclosed the presence of saturated fatty acids (SFA, 6.22-16.78%), monounsaturated fatty acids (MUFAs, 3.01-10.59%), and polyunsaturated fatty acids (PUFAs, 8.4-20.01%). The overall percentage of omega fatty acids ranged between ω3 and ω6 (5.63-14.01% and 2.77-6.1%), and the ratio of ω3/ω6 was 3.08-2.03% and ω6/ω3 was 0.32-0.49%. Moreover, n-3 PUFA was more than n-6 PUFA. In contrast, major amino acids in five fish species were glycine (4.77%), lysine (3.57%), methionine (2.91%), arginine (2.04%), and threonine (1.81%). The results obtained from this study can act as baseline data for evaluating the nutritional profiles of various fishes in the future. This is the first report of fatty and amino acids analysis on recently commercialized marine pufferfishes in the Gulf of Mannar region, and this finding may lead to new insights for further research.

Synergistic effect of novel chitosan combined metformin drug on streptozotocin-induced diabetes mellitus rat.

Metformin is widely used as a frontline medicine of type-II diabetes. Frequent overdose side-effects and their consequent adversative need to be reduced. The novel source of marine hydrozoa, Thyroscyphus ramosus derived chitosan combined metformin drug was administrated to evaluate the antidiabetic potential on a high-fat diet (HFD) with streptozotocin (STZ) induced diabetic rats. The primary analysis of In vitro antioxidant activity was tested for various solvent dissolved chitosan. Based on their IC50 dose values, CsnAA was chosen for further experiments. The chitosan was combined with metformin by sonication and confirmed through XRD, FTIR and SEM analysis. The enhanced activity was observed in 100 mg/kg metformin combined with CSN500mg/kg. The level of serum bilirubin, total protein, SGH, liver glycogen, Glucose-6-phosphatase and fructose-1,6- phosphatase values were significantly similar to metformin 300 mg/kg treated group. With this beneficiary, the novel chitosan was recommended to administrate with metformin to improve the drug efficacy and reduction of overdose lethal effects.

Muscle extract of Arothron immaculatus regulates the blood glucose level and the antioxidant system in high-fat diet and streptozotocin induced diabetic rats.

In the present study pufferfish, Arothron immaculatus muscle methanol extract (AIME) was used to evaluate the antidiabetic activity against the high-fat diet (HFD) in streptozotocin (STZ) induced diabetic rat models. Initially, the In vitro antioxidant activity of the different muscle extract was evaluated which showed that AIME has higher efficiency to scavenge the free radicals. The animal study results revealed that the AIME could decrease the blood glucose level after 14 days of oral treatment and recover the animal from the severe progression of the disease. The LC-ESI/MS analysis of AIME extract revealed the presence of compounds such as docosahexaenoic acid, adrenic acid, docosanol, codeine and metoprolol. Among these compounds, docosahexaenoic acid, adrenic acid and docosanol are reported for its antidiabetic studies. Hence, the muscle is recommended to consume by humans as natural food in order to overcome the development of diabetes. This is the first study on the muscle extract of marine pufferfish which is used as antidiabetic agent to treat the diabetes-induced in the animal model.