RESUMO
Cyanobacteria, a group of microalgae are the potent organism having the ability to survive in the copper rich environment and recently gained too much attention for their profuse proliferation in such water bodies. Amongst the members of cyanobacteria, the current study was conducted on Leptolyngbya sp. GUEco1015, collected from hydrocarbon rich water bodies of Assam, India. Morphological images of treated samples showed a remarkable damage in the cell surface as well as the organelles over the control. Biochemical results revealed a significant increase of enzymatic and non-enzymatic antioxidants during oxidative damage of Cu2+. But, ascorbate in 1.2 ppm (p < 0.01), 1.5 ppm (p < 0.001) and catalase content 1.5 ppm (p < 0.05) showed a significant reduction after a certain level. The cells were optimized to evaluate the maximum Cu2+ removal potential by the cells related to growth. Initial metal concentration 0.1 ppm, pH 7.5, temperature 25 °C and shaking rate 100 rpm are the optimized abiotic parameters which showed maximum 83% of Cu2+ removal. FTIR spectroscopy and EDX data has identified a number of notable functional groups that were involved in Cu2+ binding mechanism and revealed a distinctive peak of Cu with 0.41 wt % which makes the species as one of the competent copper adsorbents.
RESUMO
In this study, the seed endosphere of a bacterial wilt tolerant chilli cv. Firingi Jolokia was explored in order to find effective agents for bacterial wilt disease biocontrol. A total of 32 endophytic bacteria were isolated from freshly collected seeds and six isolates were selected based on R. solanacearum inhibition assay. These isolates were identified as Bacillus subtilis (KJ-2), Bacillus velezensis (KJ-4), Leuconostoc mesenteroides (KP-1), Lactococcus lactis (LB-3), Bacillus amyloliquefaciens (WK-2), and Bacillus subtilis (WK-3) by 16S rRNA gene sequencing. In the in planta R. solanacearum inhibition assay carried out by seedling root bacterization method, Bacillus subtilis (KJ-2) exhibited highest biocontrol efficacy of 86.6 % on 7th day post R. solanacearum inoculation and a minimum biocontrol efficacy of 52.9 % was noted for Leuconostoc mesenteroides (KP-1). GC-HRMS analysis detected several known antimicrobial compounds in the extract of the culture supernatant of Bacillus subtilis (KJ-2); which may contribute to inhibition of R. solanacearum. In the growth promotion assay conducted using these isolates, only two of them namely Bacillus subtilis (KJ-2) and Bacillus amyloliquefaciens (WK-2) showed growth promotion in true leafed tomato plants. All the selected seed endophytic isolates were able to control bacterial wilt of tomato at the seedling stage and Bacillus subtilis (KJ-2) was found to be most effective in controlling the disease. The results of the present study highlighted that seed endosphere of bacterial wilt tolerant cultivar is a rich source of R. solanacearum antagonizing bacterial isolates.
