[Development of a method of concentrating enteroviruses for virologic studies of meat]. / Razrabotka metoda kontsentratsii enterovirusov pri virusologicheskikh issledovaniiakh miasa.

A method for concentration of enteroviruses in meat extracts was developed using polyvinylpyrrolidone (molecular mass 10,000), polyethylene glycol (molecular mass 15,000), both in 10% concentration, silicagel with a granule size of 200 mesh in a concentration of 5%, aluminum hydroxide in an amount of centrifuged pellet of 5 ml of original suspension per 10 ml meat extract. When the optimal conditions of meat extract preparation (meat mass: Hanks' solution ration 1: 10) were observed, followed by virus concentration in polymers of silicagel 2--4 PFU of enteroviruses in 1 g of meat could be determined in all the experiments. Virus doses of 0.2--0.5 PFU per 1 g of meat could be detected in some experiments.

A method of isolating enteroviruses from meat. / K metodu vydeleniia enterovirusov iz miasa

A method of isolating the virus ECHO II in specimens of stewed beef and cutlets is assessed. The procedure involves obtaining extracts of the meat with the saline (in ratios of 1:2 and 1:4) in combination with processing with freon 113. This method failed to detect in the meat samples analyzed 20-30 CPD50 of the virus in 1 g. Experiments demonstrated that eluation of the poliovirus from the meat into an aqueous saline depended on the proportion of meat and the eluating solution. It was shown that the optimal extraction of the poliovirus from meat into the solution was seen to occur with the proportions of meat and the eluating solution of 1:8-1:9.

[Experimental data on the virological study of pasteurized milk]. / Eksperimental'nye materialy virusologicheskogo issledovaniia pasterizovannogo moloka

Virological analyses of sterile milk per se enabled the poliovirus to be disclosed with a concentration of 30 infection units in 1 ml. To determine such doses of the viruses in pasteurized milk the viruses have to be concentrated, for prior to cytopathogenic manifestations subsequent to these viral doses there occurs bacterial germination. In achieving poliovirus concentration in pasteurized milk are suitable polyethyleneglycol, molecular weight 15 000, and polyvinylpyrolidon, molecular weight 10 000, in a 10% concentration. Dilution of milk with Hanks' solution in the ratio of 1:4 and 1:9 improves the process of the poliovirus sorption-eluation with the polymer, enabling it to detect 3 BOU of the virus in 1 ml of milk. The AB-17-8 anionite and Ky-21-8 cationite are capable of adequately sorbing the poliovirus in pasteurized milk, but it is only from the cationite, and then only in a small amount, that eluation of the virus can be accomplished.