Zinc affects nuclear factor kappa b and DNA methyltransferase activity in C3H cancer fibroblast cells induced by a 2100 MHz electromagnetic field.

The use of mobile phones is becoming widespread with the development of technology, and as a result, its effects on human health are becoming more and more important every day. Studies have reported that the electromagnetic field (EMF) emitted by mobile phones may have adverse effects on the biological systems. In order to evaluate the effect of zinc (Zn) on C3H cancer fibroblast cells exposed to 2100 MHz EMF, we analyzed cell viability%, nuclear factor kappa b (NF-κB) and DNA methyltransferase (DNMT) activities. Cells were divided to following groups: Control, sham control, 2100 MHz EMF, 50 µM Zn + 2100 MHz EMF, 100 µM Zn + 2100 MHz EMF, and 200 µM Zn + 2100 MHz EMF for 2 h. We measurement cell viability, NF-κB and DNMT activities. There was increased cell viability % in the 2100 MHz EMF group compared to the control group, while the cell viability % was decreased in the 50, 100 and 200 µM Zn + 2100 MHz EMF groups compared to 2100 MHz EMF. NF-κB and DNMT activities were a significant increase in the 2100 MHz EMF group compared to the control group, although were statistically decreased in the 50, 100 and 200 µM Zn + 2100 MHz EMF groups compared to the 2100 MHz EMF group. Our results demonstrate that 2100 MHz EMF exposure in cancer fibroblast cells induce NF-κB and DNMT activities, whereas zinc supplementation reduce NF-κB and DNMT activities-induced 2100 MHz EMF.

The 50 Hz (10 mT) sinusoidal magnetic field: effects on stress-related behavior of rats.

PURPOSE: The purpose of this study was to investigate the behavioral changes induced by 50 Hz, 10 mT flux density Sinusoidal Magnetic Field (MF). MATERIAL AND METHODS: Seventy-six young adult male Wistar albino rats were used in the study. They were separated into two groups: control group (C) n=38; MF group n=38. C animals were left under the same conditions with the MF group for 21 days but with prevented or avoided exposure to MF. Anxiety and stress-related behavioral changes were investigated by elevated plus-maze and hole-board systems. Just before being tested in the maze, each animal was tested by means of the hole-board method in order to separate the directed exploration behavior and locomotion activity changes from anxiety-related behavior. RESULTS: In the hole-board system parameters there were no statistically significant differences between the two groups. There was a statistically significant difference between MF and C groups when the ratio of time spent on open arms to the total time spent on all arms was evaluated (0.12±0.08 and 0.34±0.18 respectively and p <0.01). CONCLUSION: Our results suggest that after 21 days, a continuous exposure to extremely low frequency of magnetic field (50 Hz, 10 mT) has no significant effect on activity and exploration activity but significantly induces stress and anxiety-related behavior in rats (Tab. 2, Fig. 9, Ref. 19).