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1.
Anal Chem ; 91(22): 14407-14416, 2019 11 19.
Artigo em Inglês | MEDLINE | ID: mdl-31638379

RESUMO

A challenge facing metabolomics in the analysis of large human cohorts is the cross-laboratory comparability of quantitative metabolomics measurements. In this study, 14 laboratories analyzed various blood specimens using a common experimental protocol provided with the Biocrates AbsoluteIDQ p400HR kit, to quantify up to 408 metabolites. The specimens included human plasma and serum from male and female donors, mouse and rat plasma, as well as NIST SRM 1950 reference plasma. The metabolite classes covered range from polar (e.g., amino acids and biogenic amines) to nonpolar (e.g., diacyl- and triacyl-glycerols), and they span 11 common metabolite classes. The manuscript describes a strict system suitability testing (SST) criteria used to evaluate each laboratory's readiness to perform the assay, and provides the SST Skyline documents for public dissemination. The study found approximately 250 metabolites were routinely quantified in the sample types tested, using Orbitrap instruments. Interlaboratory variance for the NIST SRM-1950 has a median of 10% for amino acids, 24% for biogenic amines, 38% for acylcarnitines, 25% for glycerolipids, 23% for glycerophospholipids, 16% for cholesteryl esters, 15% for sphingolipids, and 9% for hexoses. Comparing to consensus values for NIST SRM-1950, nearly 80% of comparable analytes demonstrated bias of <50% from the reference value. The findings of this study result in recommendations of best practices for system suitability, quality control, and calibration. We demonstrate that with appropriate controls, high-resolution metabolomics can provide accurate results with good precision across laboratories, and the p400HR therefore is a reliable approach for generating consistent and comparable metabolomics data.


Assuntos
Aminoácidos/sangue , Aminas Biogênicas/sangue , Análise Química do Sangue/estatística & dados numéricos , Lipidômica/estatística & dados numéricos , Lipídeos/sangue , Metabolômica/estatística & dados numéricos , Análise de Variância , Animais , Cromatografia Líquida de Alta Pressão/estatística & dados numéricos , Agregação de Dados , Feminino , Humanos , Limite de Detecção , Masculino , Espectrometria de Massas/estatística & dados numéricos , Metaboloma , Camundongos , Ratos , Reprodutibilidade dos Testes
2.
Anal Bioanal Chem ; 407(20): 6181-90, 2015 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-26100547

RESUMO

In Campylobacterales and related ε-proteobacteria with N-linked glycosylation (NLG) pathways, free oligosaccharides (fOS) are released into the periplasmic space from lipid-linked precursors by the bacterial oligosaccharyltransferase (PglB). This hydrolysis results in the same molecular structure as the oligosaccharide that is transferred to a protein to be glycosylated. This allowed for the general elucidation of the fOS-branched structures and monosaccharides from a number of species using standard enrichment and mass spectrometry methods. To aid characterization of fOS, hydrazide chemistry has often been used for chemical modification of the reducing part of oligosaccharides resulting in better selectivity and sensitivity in mass spectrometry; however, the removal of the unreacted reagents used for the modification often causes the loss of the sample. Here, we develop a more robust method for fOS purification and characterize glycostructures using complementary tandem mass spectrometry (MS/MS) analysis. A cationic cysteine hydrazide derivative was synthesized to selectively isolate fOS from periplasmic fractions of bacteria. The cysteine hydrazide nicotinamide (Cyhn) probe possesses both thiol and cationic moieties. The former enables reversible conjugation to a thiol-activated solid support, while the latter improves the ionization signal during MS analysis. This enrichment was validated on the well-studied Campylobacter jejuni by identifying fOS from the periplasmic extracts. Using complementary MS/MS analysis, we approximated data of a known structure of the fOS from Campylobacter concisus. This versatile enrichment technique allows for the exploration of a diversity of protein glycosylation pathways.


Assuntos
Campylobacter/química , Cisteína/análogos & derivados , Oligossacarídeos/análise , Espectrometria de Massas em Tandem/métodos , Cisteína/química , Oligossacarídeos/isolamento & purificação , Extração em Fase Sólida/métodos , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos
3.
Proteomics ; 14(4-5): 489-97, 2014 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-24339419

RESUMO

Epigenetic regulation of gene expression is, at least in part, mediated by histone modifications. PTMs of histones change chromatin structure and regulate gene transcription, DNA damage repair, and DNA replication. Thus, studying histone variants and their modifications not only elucidates their functional mechanisms in chromatin regulation, but also provides insights into phenotypes and diseases. A challenge in this field is to determine the best approach(es) to identify histone variants and their PTMs using a robust high-throughput analysis. The large number of histone variants and the enormous diversity that can be generated through combinatorial modifications, also known as histone code, makes identification of histone PTMs a laborious task. MS has been proven to be a powerful tool in this regard. Here, we focus on bottom-up, middle-down, and top-down MS approaches, including CID and electron-capture dissociation/electron-transfer dissociation based techniques for characterization of histones and their PTMs. In addition, we discuss advances in chromatographic separation that take advantage of the chemical properties of the specific histone modifications. This review is also unique in its discussion of current bioinformatic strategies for comprehensive histone code analysis.


Assuntos
Histonas/metabolismo , Espectrometria de Massas/métodos , Processamento de Proteína Pós-Traducional , Biologia Computacional , Dano ao DNA/genética , Replicação do DNA/genética , Histonas/genética , Histonas/isolamento & purificação , Humanos
4.
J Proteome Res ; 12(7): 3071-86, 2013 Jul 05.
Artigo em Inglês | MEDLINE | ID: mdl-23642296

RESUMO

Mass-spectrometry-based proteomics has evolved as the preferred method for the analysis of complex proteomes. Undoubtedly, recent advances in mass spectrometry instrumentation have greatly enhanced proteomic analysis. A popular instrument platform in proteomics research is the LTQ-Orbitrap mass analyzer. In this tutorial, we discuss the significance of evaluating and optimizing mass spectrometric settings on the LTQ-Orbitrap during CID data-dependent acquisition (DDA) mode to improve protein and peptide identification rates. We focus on those MS and MS/MS parameters that have been systematically examined and evaluated by several researchers and are commonly used during DDA. More specifically, we discuss the effect of mass resolving power, preview mode for FTMS scan, monoisotopic precursor selection, signal threshold for triggering MS/MS events, number of microscans per MS/MS scan, number of MS/MS events, automatic gain control target value (ion population) for MS and MS/MS, maximum ion injection time for MS/MS, rapid and normal scan rate, and prediction of ion injection time. We furthermore present data from the latest generation LTQ-Orbitrap system, the Orbitrap Elite, along with recommended MS and MS/MS parameters. The Orbitrap Elite outperforms the Orbitrap Classic in terms of scan speed, sensitivity, dynamic range, and resolving power and results in higher identification rates. Several of the optimized MS parameters determined on the LTQ-Orbitrap Classic and XL were easily transferable to the Orbitrap Elite, whereas others needed to be reevaluated. Finally, the Q Exactive and HCD are briefly discussed, as well as sample preparation, LC-optimization, and bioinformatics analysis. We hope this tutorial will serve as guidance for researchers new to the field of proteomics and assist in achieving optimal results.


Assuntos
Peptídeos/isolamento & purificação , Proteínas/isolamento & purificação , Proteômica/instrumentação , Espectrometria de Massas em Tandem/instrumentação , Biologia Computacional , Humanos , Proteômica/métodos , Espectrometria de Massas em Tandem/métodos
5.
Anal Chem ; 85(7): 3501-7, 2013 Apr 02.
Artigo em Inglês | MEDLINE | ID: mdl-23448339

RESUMO

Middle-down mass spectrometry (MS) combined with electron capture dissociation (ECD) represents an attractive method for characterization of proteins and their post-translational modifications (PTMs). Coupling online chromatographic separation with tandem mass spectrometry enables a high-throughput analysis, while improving sensitivity of the electrosprayed peptides and reducing sample amount requirements. However, middle-down ECD has not been thus far coupled with online chromatographic separation. In this work, we examine the feasibility of coupling middle-down ECD with online nanoflow-liqiud chromatography (nano-LC) for the analysis of large, >3 kDa, and highly modified polypeptides in a data-dependent acquisition mode. We evaluate the effectiveness of the method by analyzing peptides derived from Asp-N and Glu-C digestions of unfractionated histones from calf thymus and acid-extracted histones from HeLa, MCF-7, and Jurkat cells. Our results demonstrate that middle-down ECD is compatible with online chromatographic separation, providing high peptide and protein sequence coverage while allowing precise mapping of PTM sites. The high mass accuracy, obtained by the ICR mass analyzer, for both the precursor and product ions greatly increases confidence in peptide identification, particularly for modified peptides. Overall, for all samples examined, several histone variants were identified and modification sites were successfully localized, including single, multiple, and positional isomeric PTM sites. The vast majority of the identified peptides were in the mass range from 3 to 9 kDa. The data presented here highlight the feasibility and utility of nano-LC-ECD-MS/MS for high-throughput middle-down analysis.


Assuntos
Cromatografia Líquida/métodos , Histonas/química , Peptídeos/análise , Espectrometria de Massas em Tandem/métodos , Sequência de Aminoácidos , Animais , Bovinos , Linhagem Celular , Humanos , Modelos Moleculares , Dados de Sequência Molecular
6.
J Am Soc Mass Spectrom ; 23(10): 1729-40, 2012 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-22855421

RESUMO

Hydrogen-deficient peptide radical cations exhibit fascinating gas phase chemistry, which is governed by radical driven dissociation and, in many cases, by a combination of radical and charge driven fragmentation. Here we examine electron capture dissociation (ECD) of doubly, [M + H](2+•), and triply, [M + 2H](3+•), charged hydrogen-deficient species, aiming to investigate the effect of a hydrogen-deficient radical site on the ECD outcome and characterize the dissociation pathways of hydrogen-deficient species in ECD. ECD of [M + H](2+•) and [M + 2H](3+•) precursor ions resulted in efficient electron capture by the hydrogen-deficient species. However, the intensities of c- and z-type product ions were reduced, compared with those observed for the even electron species, indicating suppression of N-C(α) backbone bond cleavages. We postulate that radical recombination occurs after the initial electron capture event leading to a stable even electron intermediate, which does not trigger N-C(α) bond dissociations. Although the intensities of c- and z-type product ions were reduced, the number of backbone bond cleavages remained largely unaffected between the ECD spectra of the even electron and hydrogen-deficient species. We hypothesize that a small ion population exist as a biradical, which can trigger N-C(α) bond cleavages. Alternatively, radical recombination and N-C(α) bond cleavages can be in competition, with radical recombination being the dominant pathway and N-C(α) cleavages occurring to a lesser degree. Formation of b- and y-type ions observed for two of the hydrogen-deficient peptides examined is also discussed.


Assuntos
Hidrogênio/química , Espectrometria de Massas/métodos , Peptídeos/química , Sequência de Aminoácidos , Cátions/química , Elétrons , Radicais Livres/química , Gases/química , Dados de Sequência Molecular
7.
J Am Soc Mass Spectrom ; 23(2): 244-63, 2012 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-22101468

RESUMO

Gas phase fragmentation of hydrogen deficient peptide radical cations continues to be an active area of research. While collision induced dissociation (CID) of singly charged species is widely examined, dissociation channels of singly and multiply charged radical cations in infrared multiphoton dissociation (IRMPD) and electron induced dissociation (EID) have not been, so far, investigated. Here, we report on the gas phase dissociation of singly, doubly and triply charged hydrogen deficient peptide radicals, [M + nH]((n+1)+·) (n=0, 1, 2), in MS(3) IRMPD and EID and compare the observed fragmentation pathways to those obtained in MS(3) CID. Backbone fragmentation in MS(3) IRMPD and EID was highly dependent on the charge state of the radical precursor ions, whereas amino acid side chain cleavages were largely independent of the charge state selected for fragmentation. Cleavages at aromatic amino acids, either through side chain loss or backbone fragmentation, were significantly enhanced over other dissociation channels. For singly charged species, the MS(3) IRMPD and EID spectra were mainly governed by radical-driven dissociation. Fragmentation of doubly and triply charged radical cations proceeded through both radical- and charge-driven processes, resulting in the formation of a wide range of backbone product ions including, a-, b-, c-, y-, x-, and z-type. While similarities existed between MS(3) CID, IRMPD, and EID of the same species, several backbone product ions and side chain losses were unique for each activation method. Furthermore, dominant dissociation pathways in each spectrum were dependent on ion activation method, amino acid composition, and charge state selected for fragmentation.


Assuntos
Hidrogênio/química , Espectrometria de Massas/métodos , Peptídeos/química , Sequência de Aminoácidos , Aminoácidos Aromáticos/química , Cátions/química , Radicais Livres/química , Dados de Sequência Molecular
8.
Proteomics ; 12(1): 21-31, 2012 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-22065615

RESUMO

The success of a shotgun proteomic experiment relies heavily on the performance and optimization of both the LC and the MS systems. Despite this, little consideration has, so far, been given to the importance of evaluating and optimizing the MS instrument settings during data-dependent acquisition mode. Moreover, during data-dependent acquisition, the users have to decide and choose among various MS parameters and settings, making a successful analysis even more challenging. We have systematically investigated and evaluated the effect of enabling and disabling the preview mode for FTMS scan, the number of microscans per MS/MS scan, the number of MS/MS events, the maximum ion injection time for MS/MS, and the automatic gain control target value for MS and MS/MS events on protein and peptide identification rates on an LTQ-Orbitrap using the Saccharomyces cerevisiae proteome. Our investigations aimed to assess the significance of each MS parameter to improve proteome analysis and coverage. We observed that higher identification rates were obtained at lower ion injection times i.e. 50-150 ms, by performing one microscan and 12-15 MS/MS events. In terms of ion population, optimal automatic gain control target values were at 5×10(5) -1×10(6) ions for MS and 3×10(3) -1×10(4) ions for MS/MS. The preview mode scan had a minimal effect on identification rates. Using optimized MS settings, we identified 1038 (±2.3%) protein groups with a minimum of two peptide identifications and an estimated false discovery rate of ∼1% at both peptide and protein level in a 160-min LC-MS/MS analysis.


Assuntos
Fragmentos de Peptídeos/química , Espectrometria de Massas em Tandem/métodos , Cromatografia Líquida , Interpretação Estatística de Dados , Técnicas Analíticas Microfluídicas/métodos , Técnicas Analíticas Microfluídicas/normas , Melhoria de Qualidade , Reprodutibilidade dos Testes , Saccharomyces cerevisiae , Proteínas de Saccharomyces cerevisiae/química , Proteínas de Saccharomyces cerevisiae/metabolismo , Espectrometria de Massas em Tandem/normas
9.
J Biomol Tech ; 22(4): 122-6, 2011 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-22131886

RESUMO

Visualization tools that allow both optimization of instrument's parameters for data acquisition and specific quality control (QC) for a given sample prior to time-consuming database searches have been scarce until recently and are currently still not freely available. To address this need, we have developed the visualization tool LogViewer, which uses diagnostic data from the RAW files of the Thermo Orbitrap and linear trap quadrupole-Fourier transform (LTQ-FT) mass spectrometers to monitor relevant metrics. To summarize and visualize the performance on our test samples, log files from RawXtract are imported and displayed. LogViewer is a visualization tool that allows a specific and fast QC for a given sample without time-consuming database searches. QC metrics displayed include: mass spectrometry (MS) ion-injection time histograms, MS ion-injection time versus retention time, MS(2) ion-injection time histograms, MS(2) ion-injection time versus retention time, dependent scan histograms, charge-state histograms, mass-to-charge ratio (M/Z) distributions, M/Z histograms, mass histograms, mass distribution, summary, repeat analyses, Raw MS, and Raw MS(2). Systematically optimizing all metrics allowed us to increase our protein identification rates from 600 proteins to routinely determine up to 1400 proteins in any 160-min analysis of a complex mixture (e.g., yeast lysate) at a false discovery rate of <1%. Visualization tools, such as LogViewer, make QC of complex liquid chromotography (LC)-MS and LC-MS/MS data and optimization of the instrument's parameters accessible to users.


Assuntos
Análise de Fourier , Proteômica/métodos , Software , Espectrometria de Massas em Tandem/métodos , Biologia Computacional , Células HeLa , Humanos , Íons/química , Proteínas/química , Proteômica/instrumentação , Controle de Qualidade , Saccharomyces cerevisiae/química , Sensibilidade e Especificidade , Espectrometria de Massas em Tandem/instrumentação
10.
J Am Soc Mass Spectrom ; 22(12): 2209-21, 2011 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-21952776

RESUMO

Dissociation of singly charged species is more challenging compared with that of multiply charged precursor ions because singly charged ions are generally more stable. In collision activated dissociation (CAD), singly charged ions also gain less kinetic energy in a fixed electric field compared with multiply charged species. Furthermore, ion-electron and ion-ion reactions that frequently provide complementary and more extensive fragmentation compared with CAD typically require multiply charged precursor ions. Here, we investigate electron induced dissociation (EID) of singly deprotonated peptides and compare the EID fragmentation patterns with those observed in negative ion mode CAD. Fragmentation induced upon electron irradiation and collisional activation is not specific and results in the formation of a wide range of product ions, including b-, y-, a-, x-, c-, and z-type ions. Characteristic amino acid side chain losses are detected in both techniques. However, differences are also observed between EID and CAD spectra of the same species, including formation of odd-electron species not seen in CAD, in EID. Furthermore, EID frequently results in more extensive fragmentation compared with CAD. For modified peptides, EID resulted in retention of sulfonation and phosphorylation, allowing localization of the modification site. The observed differences are likely due to both vibrational and electronic excitation in EID, whereas only the former process occurs in CAD.


Assuntos
Espectrometria de Massas/métodos , Peptídeos/química , Sequência de Aminoácidos , Colecistocinina/química , Elétrons , Íons/química , Dados de Sequência Molecular , Prótons , Substância P/química
11.
Mol Biosyst ; 6(9): 1668-81, 2010 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-20485823

RESUMO

Electron capture dissociation (ECD), which provides more extensive sequence coverage compared to "slow heating" tandem mass spectrometric techniques such as collision-activated dissociation, constitutes a promising method for de novo sequencing of peptides and proteins. We have previously examined and characterized the ECD fragmentation behavior of small to medium size doubly and triply protonated peptides from trypsin, chymotrypsin and Glu C digestion. Here, we extend that work to longer, more highly charged proteolytic peptides from Lys N, Lys C and Glu C digestion to compare the extent of fragmentation obtained from each type of proteolytically derived peptide and also to examine the effectiveness of each enzyme in terms of peptide sequence coverage in ECD. Our findings demonstrate that medium size peptides (i.e., 1600-4800 Da) at high charge states (+3 to +6) exhibit very similar ECD fragmentation behavior independent of which proteolytic enzyme was used for digestion. The average peptide sequence coverage we obtained for Lys N and Glu C generated peptides was 81%, and for Lys C proteolytic peptides we obtained an average sequence coverage of 80%. Most of the peptides we examined, 82%, showed high ECD sequence coverage ranging from 70-100%, whereas 13% of the peptides showed a moderate sequence coverage ranging from 50-60%. Only 5% of the peptides showed an average sequence coverage below 50%. Furthermore, the extent of fragmentation, measured by the total number of backbone c- and z-type product ions, was very similar for Lys N, Lys C and Glu C derived peptides.


Assuntos
Peptídeos/química , Espectrometria de Massas em Tandem/métodos , Quimotripsina/química , Ácido Glutâmico/química , Lisina/química , Tripsina/química
12.
Appl Environ Microbiol ; 75(5): 1381-7, 2009 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-19114522

RESUMO

The peptide antibiotic nisin A belongs to the group of antibiotics called lantibiotics. They are classified as lantibiotics because they contain the structural group lanthionine. Lanthionines are composed of a single sulfur atom that is linked to the beta-carbons of two alanine moieties. These sulfur atoms are vulnerable to environmental oxidation. A mild oxidation reaction was performed on nisin A to determine the relative effects it would have on bioactivity. High-mass-accuracy Fourier transform ion cyclotron resonance mass spectrometry data revealed the addition of seven, eight, and nine oxygens. These additions correspond to the five lanthionines, two methionines, and two histidines that would be susceptible to oxidation. Subsequent bioassays revealed that the oxidized form of nisin A had a complete loss of bactericidal activity. In a competition study, the oxidized nisin did not appear to have an antagonistic affect on the bioactivity of nisin A, since the addition of an equal molar concentration of the oxidized variant did not have an influence on the bactericidal activity of the native antibiotic. Electron microscopy data revealed that the oxidized forms were still capable of assembling into large circular complexes, demonstrating that oxidation does not disrupt the lateral assembly mechanism of the antibiotic. Affinity thin-layer chromatography and fluorescence microscopy experiments suggested that the loss of activity is due to the inability of the oxidized form of nisin to bind to the cell wall precursor lipid II. Given the loss of bioactivity following oxidation, oxidation should be an important factor to consider in future production, purification, pharmacokinetic, and pharmacodynamic studies.


Assuntos
Alanina/análogos & derivados , Antibacterianos/metabolismo , Antibacterianos/farmacologia , Nisina/metabolismo , Nisina/farmacologia , Sulfetos/metabolismo , Alanina/metabolismo , Substâncias Macromoleculares , Espectrometria de Massas , Testes de Sensibilidade Microbiana , Micrococcus luteus/efeitos dos fármacos , Microscopia Eletrônica , Modelos Moleculares , Oxirredução , Staphylococcus aureus/efeitos dos fármacos , Streptococcus pneumoniae/efeitos dos fármacos , Uridina Difosfato Ácido N-Acetilmurâmico/análogos & derivados , Uridina Difosfato Ácido N-Acetilmurâmico/metabolismo
13.
J Proteome Res ; 7(7): 2834-44, 2008 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-18549259

RESUMO

In bottom-up proteomics, proteolytically derived peptides from proteins of interest are analyzed to provide sequence information for protein identification and characterization. Electron capture dissociation (ECD), which provides more random cleavages compared to "slow heating" techniques such as collisional activation, can result in greater sequence coverage for peptides and proteins. Most bottom-up proteomics approaches rely on tryptic doubly protonated peptides for generating sequence information. However, the effectiveness, in terms of peptide sequence coverage, of tryptic doubly protonated peptides in ECD remains to be characterized. Herein, we examine the ECD fragmentation behavior of 64 doubly- and 64 triply protonated peptides (i.e., a total of 128 peptide ions) from trypsin, Glu-C, and chymotrypsin digestion in a Fourier transform ion cyclotron resonance mass spectrometer. Our findings indicate that when triply protonated peptides are fragmented in ECD, independent of which proteolytic enzyme was used for protein digestion, more c- and z-type product ions are observed, and the number of complementary fragment pairs increases dramatically (44%). In addition, triply protonated peptides provide an increase (26%) in peptide sequence coverage. ECD of tryptic peptides, in both charge states, resulted in higher sequence coverage compared to chymotryptic and Glu-C digest peptides. The peptide sequence coverage we obtained in ECD of tryptic doubly protonated peptides (64%) is very similar to that reported for electron transfer dissociation of the same peptide type (63%).


Assuntos
Quimotripsina/química , Fragmentos de Peptídeos/química , Serina Endopeptidases/química , Tripsina/química , Sequência de Aminoácidos , Animais , Análise de Fourier , Dados de Sequência Molecular , Prótons , Espectrometria de Massas em Tandem
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