RESUMO
A comparative study of the excess of Zn, Fe and Pb on the amount of goblet cells (GC) and localization of these cations in the chick small intestine epithelium was carried out. In chicks with additional dose of Zn (2 g/kg) and Pb (0.2 g/kg), compared to control chicks the amount of GC in the epithelium of various intestinal parts increased by 1.5-1.8 times. Under a high dose of Pb (2.0 g/kg), the amount of GC increased by 3-4 times, and degenerative changes in the epithelium were observed. A histochemical electron microscopical study of duodenal sections revealed an accumulation of Fe, Zn and Pb within GC and in their excretion, in addition to brush border and glycocalyx. The involvement of intestinal GC in cation excretion is proven.
Assuntos
Intestino Delgado/metabolismo , Ferro/metabolismo , Chumbo/metabolismo , Zinco/metabolismo , Animais , Contagem de Células/efeitos dos fármacos , Galinhas , Células Epiteliais , Epitélio/metabolismo , Histocitoquímica , Intestino Delgado/citologia , Ferro/administração & dosagem , Chumbo/administração & dosagem , Microscopia Eletrônica , Zinco/administração & dosagemRESUMO
A comparative study on the amount of goblet cells and excretion of endogenic calcium in the small intestine of chicks has been made. The amount of goblet cells (per brush area unit) significantly increased at the deficiency of vitamin D3 as well as at the surplus of calcium in the diet. Excretion intensity of endogenic calcium (determined by 45Ca) correlates directly with the amount of goblet cells. Histochemical and electron microscopical investigations show that precipitate accumulations containing calcium are absorbed in goblet cells and their secret on the surface of the cells. The significance of goblet cells in the intestinal excretion of endogenic calcium is discussed.