Treatment of chronic osteomyelitis using the Papineau technique.

From 1984 to 1994, 41 cases of chronic osteomyelitis were treated in Kinshasa by the Papineau technique; 75.5% were men and 24.5% women. The mean age was 28 years (range: 7-77), and the average duration of the infection was 3 years (range: 1 month to 28 years). The aetiology was "trauma" in 53.5%, haematogenous in 44% and drepanocytosis in 2.5%. Infections of the femur and tibia each constituted 41.5% of the cases. Immobilisation was by external fixation in 44%, and by a cast in 39%. Wound healing was complete after an average of 3 months with spontaneous healing, and in 4.5 months after skin grafting. Control of the infection and bone healing were obtained in 89% after a period of 3-7 months. We discuss the Papineau technique and the modifications which were required in Kinshasa.

Knock down and survival of tsetse flies fed on cattle and pigs dipped in deltamethrin.

Glossina morsitans, G. pallidipes and G. fuscipes fuscipes were fed on cattle or pigs that had been dipped in 0.00375% deltamethrin in water, 0-31 days previously. The knock down and survival of the tsetse were then followed in the laboratory. Although mortality was generally less the longer after the dip the flies were fed, all those that fed on the animals within 7 days of the dipping were killed and all those that fed within 21 days were at least knocked down. Glossina morsitans was slightly more susceptible than the other two species tested.

Kasokero virus: a new human pathogen from bats (Rousettus aegyptiacus) in Uganda.

Two virus strains were isolated by mouse inoculation from blood of Rousettus aegyptiacus fruit-eating bats collected from Kasokero Cave in Uganda. Shortly after these strains were introduced in the laboratory, four additional strains were recovered from laboratory workers who had developed mild to severe illnesses presumably as a result of laboratory infection. Serological studies established that these six isolates are strains of the same virus. Serological tests showed also that this virus is related to Yogue, an unclassified virus.

Malignant catarrhal fever III. Experimental infection of sheep, domestic rabbits and laboratory animals with malignant catarrhal fever virus.

Five of 19 sheep became infected when inoculated with a virulent strain of malignant catarrhal fever virus isolated in Kenya One of the infected animals was killed in extremis; its blood and lymph node suspension reproduced the classical disease in three steers. Calves exposed to these sheep did not become infected during 89 days of close contact. The Kenya strain of malignant catarrhal fever virus infected rabbits, guinea pigs and hamsters, producing occular and nasal discharges, paralysis and death. The virus recovered from these animals in cell cultures produced disease in rabbits and steers. Neutralizing antibodies were found in the rabbit sera. Infant mice, chicken and duck embryos were refractory to infection with malignant catarrhal fever virus.

Malignant catarrhal fever. I. Response of American cattle to malignant catarrhal virus isolated in Kenya.

Fifty-three American cattle were inoculated with malignant catarrhal fever virus isolated from a wildebeest in Kenya. Three animals showed the mild form of the disease and recovered, and 47 showed the severe form of the disease. The other three did not react. Of the 47 cattle, 28 died, 16 were killed for the collection of specimens and three recovered. The incubation period for the 47 cattle ranged from 16 to 29 days and the course of the fatal disease for 28 cattle averaged three to 23 days. Virus titration of specimens from nine infected steers yielded a mean titer of 10(4)/TCID50 per gm for lymph nodes, 10(3) TCID50 per mL for buffy coats and 10(2.3) TCID50 per gm for spleens. Smaller amounts of virus were found in the liver, kidneys, adrenals and thyroids. Malignant catarrhal fever virus was also found in nasal secretions and saliva of viremic cattle. Viral infectivity was shown in bovine buffy coat cells stored at 4 degrees C for two days but was immediately destroyed upon freezing even when glycerine or dimethylsulfoxide was added. Viral particles were not found in infected animal tissues by electron microscopy. The disease was successfully transmitted in steers by intratracheal intubation and by aerosol inhalation but not by contact.

The production and use of rinderpest cell culture vaccine.

Description of preparation, safety and efficacy testing of a rinderpest cell culture vaccine which is observed in lyophilized form and should be used within two hours following its reconstitution. Excellent results have been obtained with this vaccine in Kenya, Uganda and Tanzania.

Immunisation of cattle against the herpesvirus of malignant catarrhal fever: failure of inactivated culture vaccines with adjuvant.

Attempts were made to immunise cattle against the herpesvirus of malignant catarrhal fever by inoculating living or formalinised preparations of the agent, propagated in cell cultures and combined with Freund's incomplete adjuvant. High and persistent levels of virus-neutralising antibody were regularly demonstrable, especially following two intramuscular inoculations at an interval of six to eight weeks. In spite of this no protection was demonstrable against parenteral challenge with virulent virus, whether in cell-free or cell-associated form. In a controlled field trial vaccinated cattle showed no evidence of protection against natural challenge by exposure to wildebeest herds. It was concluded that humoral mechanisms are probably not important in determining resistance to infection with virulent MCFV.

Serologic classification of feline caliciviruses by plaque-reduction neutralization and immunodiffusion.

Serologic classification of 14 isolated of feline caliciviruses was carried out, using plaque-reduction neutralization tests. Sixty to 200 plaque-forming units of virus were employed against 20 antibody units of hyperimmune goat antiserums prepared to individual isolates. The results established that these viruses were related in various degrees, but no 2 were identical. Arrangements in order of the greatest intergroup reactions yielded essentially 1 serotype with the exception of 1 virus showing 1-way neutralization reactions with heterologous isolates. The geographic origin, the site of viral isolation, or the plaque size had no relationship with the neutralization pattern. The F-9 isolate that showed the greatest intergroup reactions was proposed as the possible reference virus. The viruses were indistinguishable by using gel-diffusion analysis against cat antiserums.