RESUMO
Mutations in the rpoB, katG, inhA, oxyR/ahpC genes in rifampicin- and isoniazid-resistant M. tuberculosis strains isolated from residents of Moscow, Astrakhan', and Moldova Republic were studied by molecular biological methods (heteroduplex analysis, single strand conformational polymorphism, biochips). Twenty-five combinations of mutations were detected. Some differences in the type distribution of detected mutations were found. The use of biochips is the most perspective method for determining the type of mutation.
Assuntos
Técnicas de Tipagem Bacteriana , Isoniazida/farmacologia , Mycobacterium tuberculosis/metabolismo , Rifampina/farmacologia , Antibióticos Antituberculose/farmacologia , Antituberculosos/farmacologia , Códon , DNA/metabolismo , Resistência Microbiana a Medicamentos , Humanos , Mutação , Ácidos Nucleicos Heteroduplexes , Análise de Sequência com Séries de Oligonucleotídeos , Polimorfismo Conformacional de Fita Simples , Tuberculose/tratamento farmacológico , Tuberculose/microbiologiaRESUMO
Therapy of experimental leprosy with dried and grated horseradish root administered perorally in a dose of 300 mg/kg mixed food and treatment with purified horseradish peroxidase increased myeloperoxidase activity of blood neutrophils, enhanced antimicrobial functions of phagocytes, decreased leukocytosis, normalized total blood cell count, and produced no adverse effects on the functional state of the liver in mice.
Assuntos
Armoracia/química , Contagem de Células Sanguíneas , Hanseníase/imunologia , Fígado/efeitos dos fármacos , Fagócitos/efeitos dos fármacos , Raízes de Plantas/química , Animais , Relação Dose-Resposta a Droga , Hanseníase/fisiopatologia , Fígado/imunologia , Fígado/fisiopatologia , Camundongos , Camundongos Endogâmicos CBA , Fagócitos/imunologiaRESUMO
Therapy of experimental leprosy with dried and grated horseradish root administered perorally in a dose of 300 mg/kg mixed food and treatment with purified horseradish peroxidase increased myeloperoxidase activity of blood neutrophils, enhanced antimicrobial functions of phagocytes, decreased leukocytosis, normalized total blood cell count, and produced no adverse effects on the functional state of the liver in mice.
Assuntos
Animais , Camundongos , Armoracia/química , Camundongos Endogâmicos CBA , Contagem de Células Sanguíneas , Fagócitos , Fagócitos/imunologia , Fígado , Fígado/fisiopatologia , Fígado/imunologia , Hanseníase/fisiopatologia , Hanseníase/imunologia , Raízes de Plantas/química , Relação Dose-Resposta a DrogaAssuntos
Peroxidase do Rábano Silvestre/uso terapêutico , Hansenostáticos/uso terapêutico , Hanseníase/tratamento farmacológico , Mycobacterium leprae , Administração Oral , Animais , Dapsona/uso terapêutico , Relação Dose-Resposta a Droga , Contagem de Eritrócitos , Hemoglobinas/análise , Histocitoquímica , Imunidade Celular , Hansenostáticos/administração & dosagem , Hanseníase/imunologia , Hanseníase/microbiologia , Contagem de Leucócitos , Linfócitos/imunologia , Camundongos , Monócitos/imunologia , Neutrófilos/imunologia , Peroxidase/biossíntese , Peroxidase/imunologia , Fagócitos/imunologia , Fatores de TempoRESUMO
Therapeutic effect of lyophilized horseradish peroxidase in complex with the basic antileprosy drugs diaminodiphenylsulfone and rifampicin was studied in experimental leprosy. Oral therapy with drug complexes was more effective than monotherapy. Treatment with drug combinations activated myeloperoxidase in blood neutrophil, produced an antiinflammatory effect, stimulated cell immunity, and had no toxic effect on mouse liver.
Assuntos
Hansenostáticos/farmacologia , Hanseníase/tratamento farmacológico , Fígado/efeitos dos fármacos , Peroxidase/farmacologia , Fagócitos/efeitos dos fármacos , Animais , Anti-Inflamatórios/farmacologia , Dapsona/farmacologia , Combinação de Medicamentos , Peroxidase do Rábano Silvestre/metabolismo , Camundongos , Camundongos Endogâmicos CBA , Neutrófilos/efeitos dos fármacos , Rifampina/farmacologia , Fatores de TempoRESUMO
Lyophilized horseradish peroxidase (activity 100 U/mg) administered per os in a dose of 100-200 mg/kg fodder enhanced bactericidal activity of phagocytes in mice experimentally infected with Mycobacterium leprae, which manifested in suppression of M. leprae growth in comparison with untreated controls.
