[Digestive enzyme functionality in pathology of the gastrointestinal tract with intestinal microbiocenosis disorders].

A change pattern in enzyme release function of digestive glands exerts the organism as a whole. Dysfunctions of hydrolysis and intestinal malabsorption (secondary malabsorption syndrome) are the first step towards nutrition and metabolism processes abnormality and that can play a role in genesis of pathological conditions. Recent researches arouse clinicians interest in determination of biofluid enzyme activity in different physiological and abnormal cases. Intestinal infections are followed by dysbacteriosis and obvious alterations in the hydrolase levels in the blood, urine and motions because of the changes of functional status of the liver, renal and intestinal barriers in relation to increted digestive enzymes. This causes an unfavorable course of recovery with the development of postinfectious digestion disorders as a result of previous acute diarrhea. Future researches are necessary to elaborate appropriate remodeling of developed pathosis with the help of enzymotherapy and probiotic diet.

[A procedure for determining the proteolytic activity of biological substrates at various pH values].

The proteolytic activity of biosubstrates and biological fluids at different pH values was determined in the third trimester of pregnancy and at labor. The nature of their proteolytic activity was specified for each biological fluid and it can be associated with pepsin or other proteases.

Crystallography of biological fluid as a method for evaluating its physicochemical characteristics.

Using an integral qualitative and quantitative approach to the studies of initiation of the biological material crystallogenesis, we showed in experiments with normal human saliva that the external characteristics of biological fluid (pH, osmolality, and environmental temperature) determine the results of crystallization (tesigraphic facies). The main external (macroenvironment) and inner (microenvironment) factors of biological fluid crystal formation, determining specific features of the tesigraphic facies, were distinguished and classified. The informative value of differential analysis of biomaterial properties by means of modulating the environmental conditions is established.

[Anabolic effects of parenterally administered gastric gland hydrolases]. / Anabolicheskie vliianiia parenteral'no vvodimykh gidrolaz pishchevaritel'nykh zhelez.

In rats, i.v. administration of amylase, pepsinogen, and tripsinogen in microdoses increased the incretion of radiomethyonin from the blood into the tissue proteins of some organs. The incretion of pepsinogen stimulated the protein-production function of pancreas, tripsinogen stimulated the function of stomach glands, amylase--the function of the liver and the small intestine mucosa. The labeled pepsinogen 125I concentrated mainly in secretory organs.

[Secretion of pepsinogen, amylase and alkaline phosphatase by human sweat glands]. / Vydelenie potovymi zhelezami cheloveka pepsinogena, amilazy i shchelochnoi fosfatazy

Human sweat contains pepsinogen, amylase, and alkaline phosphatase excretion of which depends on functional state of the digestive and sweat glands and on the topography of latter. Pepsinogen excreted with sweat has the gastric origin. Different food irritants activate amylase and alkaline phosphatase excretion with sweat. A high positive correlation exists between amylase and pepsinogen excretion by kidneys and sweat glands in healthy subjects.