Molecular epidemiology of Sporothrix schenkii isolates in Malaysia.

Sporothrix schenkii is a dimorphic fungus that causes infections in both humans and animals. We report on 25 S. schenkii isolates collected in 2017 from humans and cats clinically diagnosed with sporotrichosis, in Malaysia. These isolates were phenotypically identified as S. schenkii sensu lato and further defined as S. schenckii sensu stricto based on partial calmodulin gene sequence. Isolates from both humans and cats were genotypically identical but displayed phenotypic variation. Phylogenetic analyses based on partial calmodulin sequence showed that the Malaysian isolates clustered with global S. schenkii sensu stricto strains, in particular, of the AFLP type E. This analysis also revealed that partial calmodulin sequence alone was sufficient for classifying global S. schenckii sensu stricto strains into their respective AFLP types, from A to E. The genetically conserved S. schenkii sensu stricto species isolated from humans and cats is suggestive of a clonal strain present in Malaysia. To the best of our knowledge, this is the first report on molecular identification of Sporothrix schenkii strains from human infections in Malaysia. Further studies are required in order to elucidate the clonal nature of Malaysian S. schenkii isolates. Our findings indicate the presence of a predominant S. schenkii genotype in the environment, causing infections in both cats and humans in Malaysia.

The effect of increasing the illumination on operators' visual performance in the control-room of a combined cycle power plant

BACKGROUND: Lighting is one of the environmental factors affecting the performance of the control room operators. Therefore, the aim of this study was to compare the control room operators’ visual performance in two different illumination conditions at a combined cycle power plant through field-work. METHODS: Sixteen operators in day shift were evaluated with the Freiburg Visual Acuity test (FrACT) software at two lighting systems with different intensities. It includes the first phase with fluorescent illumination system (Power: 40 W, Color Temperature: 4000 Kelvin, Luminous Flux: 2780 Lumen and Model: Pars Shahab) and the second phase with a combined illumination system includes fluorescent and LED (Power: 48 W, Color Temperature: 4000 Kelvin, Luminous Flux: 5400 Lumen and Model: Mazinoor). RESULTS: Based on the results, visual performance index and visual acuity significantly increased after the intervention (p < 0.001). As to contrast, more lighting significantly reduced the percentage of recognized contrast (p < 0.001) and increased the contrast performance index (p < 0.001). CONCLUSIONS: The results of this study showed that increasing the intensity of light from the values below the allowable limit to the values above the allowable limit would increase the visual indicators in individuals.

Distribution of ticks (Acari: Ixodidae) infesting domestic ruminants in mountainous areas of Golestan province, Iran

<p><b>OBJECTIVE</b>To determine the prevalence of ticks on cattle in the mountainous areas of Golestan province and their geographical distribution.</p><p><b>METHODS</b>In total, 498 animals from 25 herds were selected to search for ticks in 2009-2010. Tick collection was carried out during four seasons, twice per season over a period of 12 month from March 2009 through February 2010 in two districts, Azadshahr and Ramian. Meteorological data were obtained from Iran Meteorological Organization. The geographical points recorded using a Garmin eTrex®H GPS.</p><p><b>RESULTS</b>A total of 255 ticks were collected from a total of 219 ruminants including 44 sheep, 63 goats, 99 cows and 13 camels in two districts of the mountainous area of Golestan province, including Azadshahr and Ramian. Five species of ixodid ticks were identified: Rhipicephalus sanguineus (66.5%), Rhipicephalus bursa (4.6%), Hyalomma marginatum (19.9%), Hyalomma anatolicum (6%) and Hyalomma asiaticum (4%). The densities of infestations were calculated for sheep, goats, cows and camels 0.9, 0.79, 0.16 and 0.43 respectively. Seasonal activity of each ixodid tick infesting domestic ruminants was determined. The distribution maps showed ixodid ticks on domestic ruminants, and Rhipicephalus sanguineus were dominant species in the area.</p><p><b>CONCLUSIONS</b>Such research provides necessary information for human and animal health service mangers to have a better understanding of prevention and control of vector borne diseases especially during the outbreaks.</p>