RESUMO
A Gram-negative, strictly aerobic, motile, filamentous and viscous exopolymer-producing bacterium, designated strain YT21(T), was isolated from fresh water. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain YT21(T) lies within a cluster containing the established genera Segetibacter, Terrimonas, Niastella and Chitinophaga in the phylum Bacteroidetes. However, the isolate represented a lineage distinct from these genera, with sequence similarities ranging from 88.9 to 91.8 %. The genomic G+C content was 45.2 mol%. The predominant isoprenoid quinone was MK-7. The major fatty acids were 15 : 0 iso, 17 : 0 iso 3-OH and 15 : 1 iso G. On the basis of morphological features and phenotypic and phylogenetic data, strain YT21(T) represents a novel genus and species, for which the name Filimonas lacunae gen. nov., sp. nov. is proposed. The type strain of Filimonas lacunae is strain YT21(T) (=NBRC 104114(T) =DSM 21054(T)).
Assuntos
Bacteroidetes/classificação , Água Doce/microbiologia , Técnicas de Tipagem Bacteriana , Bacteroidetes/genética , Bacteroidetes/isolamento & purificação , Bacteroidetes/fisiologia , Composição de Bases , DNA Bacteriano/análise , DNA Ribossômico/análise , Ácidos Graxos/análise , Genes de RNAr , Dados de Sequência Molecular , Fenótipo , Filogenia , Quinonas/análise , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Especificidade da EspécieRESUMO
Although some bacteria require an atmosphere with high CO(2) levels for their growth, CO(2) is not generally supplied to conventional screening cultures. Here, we isolated 84 bacterial strains exhibiting high-CO(2) dependence. Their phylogenetic affiliations imply that high-CO(2) culture has potential as an effective method to isolate unknown microorganisms.
