RESUMO
To promote the use of marine microalgae for nutraceuticals, we aimed to characterize extracts of Nannochloropsis oculata, Porphyridium cruentum, and Skeletonema costatum, all of which harbor numerous bioactive substances. Chlorophylls and carotenoids were identified as the primary pigments in N. oculata and S. costatum extracts. Furthermore, the total phenolic and total flavonoid contents in the three microalgae ranged 20.32-21.96 mg GAE/g and 0.3-2.1 mg QE/g, respectively. Notably, the extract of N. oculata exhibited the most significant radical scavenging activity in both 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assays, with flavonoids and pigments identified as the main contributors to antioxidant activities. Our results revealed variations in metabolite profiles among the microalgal extracts: N. oculata extract (43 types), P. cruentum (13 types), and S. costatum (21 types). Hexadecanamide emerged as the major metabolite detected in all microalgae. Collectively, the results of the present study may open new avenues of microalgae for various applications.
