Linkage of blood pressure, obesity and diabetes mellitus with angiotensinogen gene (AGT 704T>C/rs699) polymorphism in hypertensive patients.

AIMS: The study aim was to analyse the frequency of polymorphic variants of angiotensinogen gene polymorphism (AGT 704T>C, rs699) in essential arterial hypertension (EAH) patients. METHODS: Seventy-two individuals with EAH and hypertension­mediated organ damage (stage 2), moderate, high or very high cardiovascular risks were involved in the case-control study. Among them, 70.84 % (51) were females and 29.16 % (21) were males; mean age was 59.87±7.98 y. The control group consisted of fifty practically healthy individuals at relevant age (49.13±6.28 y) and with relevant sex distribution (62 % were females, 38 % were males). AGT (704T>C) gene polymorphism was examined by RT-PCR. RESULTS: The distribution of genotypes in the study group was as follows: TT - 14 %, TC - 60 %, CC - 26 %, which corresponded to the distribution in the control group - 16 %, 54 % and 30 %, respectively, and did not deviate from the Hardy-Weinberg equilibrium. Smoking, type 2 diabetes mellitus (DM2) and obesity increased the relative risk of EAH in the examined population 2.5 times [OR=2.81; p=0.049], 3.75 times [OR=4.68; p=0.005] and almost twofold [OR=2.90; p=0.004], respectively. The probability of EAH increases fourfold with the angiotensin II elevation in the serum. Genotypes and alleles of the AGT (704T>C) gene were not significant risk factors for EAH and DM2 in the studied population. However, the TC-genotype (lesser T-allele) increases the risk of obesity in EAH patients more than 1.5 times [OR=2.93; p=0.03]. In addition, the T-allele increases the risk for blood pressure (BP) to elevate up to grade 2-3 [OR=3.64; p < 0.001]. CONCLUSIONS: One-way ANOVA analysis confirmed the AGT (704T>C) gene polymorphism to be associated with systolic and diastolic BP elevation (F=7.80; p < 0.001 and F=4.90; p=0.01, respectively), especially in TT-genotype carriers (p < 0.05), and with body mass index increase, albeit only in women (F=13.94; p < 0.001) (Tab. 4, Fig. 3, Ref. 26). Text in PDF www.elis.sk Keywords: angiotensinogen gene (AGT 704T>C), diabetes mellitus type 2, arterial hypertension, obesity, risk.

[Peculiarities of microbiological and immunological indices of human nose mucosa in conditions of man-made pollution].

Possible influence of man-made load on formation and function of microbiocenosis of the nose mucosa in practically healthy people of industrial city has been studied. Microbiota composition, content of secretory immunoglobulin A, functional state and expression of TLR-2 and TLR-4 by mucosa cells were studied in the given human biotope. The residing in conventionally contaminated districts of the industrial city tells on the increase of ecologic role of Candida albicans in the composition of microbiocenosis of the nose mucosa. The authors have shown high sensitivity of the number of TLR-4-positive epithelial cells to the type of the basic taxon of a microorganism in the composition of the nose mucosa microbiota and also sensitivity of the number of TLR-2 and TLR-4-positive epithelial cells and the density of expression by TLR-4 epithelial cells to the man-made pollution, they have also established the expressed unbalance of the system of inherent immunity of the nose mucosa of practically healthy young persons in conditions of man-made pollution.

[Searching for ways to correct thymic dysfunction in rats with experimental diabetes mellitus].

We investigated the influence of multiple introductions of NO precursor L-arginine and NOS non-selective blocker N-nitroL-arginine (NNLA) on thymic morpho-functional status in Wistar male rats with experimental diabetes mellitus (EDM). To reveal insulin-expressing, proliferating, Treg-cells, iNOS(+)-cells and Bcl-2(+)-cells, the immunohistochemical methods of direct and indirect immunofluorescence with monoclonal antibodies to insulin, PCNA, CD-25 antigen. Bcl-2 and iNOS of rat were used. It was established that NNLA administration to rats with EDM has more pronounced effect in comparison with L-arginine administration, demonstrating an increase in the number of Treg-cells, insulin-expressing and proliferating thymocytes and a decrease in the density of iNOS(+)- and Bcl-2(+)-cells population.