RESUMO
During immunoelectrophoresis in the presence of tween-80, triton X-100 and ammonium sulfate blood serum beta-glycoprotein of pregnant rats migrated along with beta-globulins as a main single band; its minor components in zones of alpha- and gamma-globulins were not detected. beta-glycoprotein was completely absorbed by phenyl sepharose in the absence of ligand as well as when the spacer arm for phenyl group was short. When the phenyl group was linked with the template through a long spacer arm, three froms of beta-glycoprotein with different immunoelectrophoretic mobility were detected after absorbtion with phenyl sepharose. Hence, beta-glycoprotein is hydrophobic and is represented by alpha-, beta- and gamma-forms in blood plasma of pregnant rats.
Assuntos
Proteínas da Gravidez/farmacologia , Prenhez/sangue , Glicoproteínas beta 1 Específicas da Gravidez/farmacologia , alfa-Globulinas/análise , Animais , beta-Globulinas/análise , Cromatografia de Afinidade/métodos , Feminino , Imunoeletroforese Bidimensional/métodos , Gravidez , Glicoproteínas beta 1 Específicas da Gravidez/análise , Glicoproteínas beta 1 Específicas da Gravidez/isolamento & purificação , Ratos , gama-Globulinas/análiseRESUMO
It has been demonstrated that pregnancy-specific beta1-globulin is synthesized by the rat placenta. Other organs of pregnant animals (liver, kidneys, lungs, heart, spleen) were incapable of synthesizing this antigen. The greatest amount of pregnancy-specific beta1-globulin is observed in the blood of intact animals on the 11th day after the introduction of ground placental tissue.
Assuntos
Placenta/metabolismo , Proteínas da Gravidez/sangue , Glicoproteínas beta 1 Específicas da Gravidez/sangue , Animais , Feminino , Placenta/imunologia , Gravidez , RatosRESUMO
It has been demonstrated that placenta extract of rats contains up to 14 antigens. Moreover, 11 of them are interorgan proteins of wide and limited specificity, two antigens (alpha 1- and alpha 2-globulins) are attributed to acute-phase proteins typical for pregnancy. beta 1-Globulin is a specific protein of rat placenta. The content of these antigens in blood serum increases with pregnancy and reaches a maximum toward the delivery; 3-4 days after delivery beta 1-globulin disappears completely from maternal blood, whereas the concentration of acute-phase proteins drops to the initial level.