RESUMO
Stable water-soluble complexes of Cd-free ZnSe/ZnS quantum dots (QDs) and chlorin e6 complexes have been prepared. These complexes have shown approximately 50% intracomplex fluorescence resonance energy transfer from QDs to chlorin e6. The photodynamic therapy (PDT) test of the complexes against the Erlich acsite carcinoma cell culture demonstrated a two-fold enhancement of the cancer cell photodynamic destruction as compared to that of free chlorin e6 molecules. It was shown that the PDT effect was significantly increased due to two factors: the efficient QD-chlorin e6 photoexcitation energy transfer and the improvement of cellular uptake of the photosensitizer in the presence of ZnSe/ZnS QDs.
Assuntos
Neoplasias/tratamento farmacológico , Fotoquimioterapia , Fármacos Fotossensibilizantes/uso terapêutico , Porfirinas/uso terapêutico , Pontos Quânticos , Animais , Clorofilídeos , Feminino , Transferência Ressonante de Energia de Fluorescência , Masculino , Camundongos , Pontos Quânticos/química , Células Tumorais CultivadasAssuntos
Muramidase/metabolismo , Derivados de Benzeno/química , Derivados de Benzeno/metabolismo , Derivados de Benzeno/farmacologia , Catálise , Quitina/metabolismo , Meios de Cultura , Relação Dose-Resposta a Droga , Ativação Enzimática , Estabilidade Enzimática , Hidrólise , Micrococcus luteus/química , Micrococcus luteus/metabolismo , Muramidase/antagonistas & inibidores , Peptidoglicano/metabolismo , Saccharomyces cerevisiae/química , Saccharomyces cerevisiae/metabolismoRESUMO
In modeling acute diphtheric toxemia in guinea pigs, the authors propose to perform hemosorption using selective sorbents under continuous introduction of antitoxic immune sera into extracorporeal contour in front of sorption column. Combination of hemosorption with serotherapy in the same time interval may be denoted as serosorption. As specific sorbents, affine preparations imasorb A-700 and Imasorb G-700 were used. They selectively eliminate from the blood flow CIC produced by diphtheric toxin (anatoxin) and antitoxic rabbit and horse antibodies. Changes in the titers of the diphtheric toxin (anatoxin) and CIC in blood evidence for efficiency of selective sorbents were confirmed by immunohistochemical analysis of the preparations' granules after hemoperfusion.
Assuntos
Difteria/terapia , Hemoperfusão/métodos , Sefarose/análogos & derivados , Toxemia/terapia , Animais , Proteínas de Bactérias , Difteria/imunologia , Toxoide Diftérico/sangue , Feminino , Cobaias , Soros Imunes , Ligantes , Masculino , Proteínas Recombinantes , Staphylococcus aureus , Streptococcus , Toxemia/imunologiaRESUMO
The antitoxic antidiphtheric immunosorbent DATs was tested in experiments on mice and guinea-pigs using the model of acute diphtheric toxemia. In extracorporeal connecting with the circulation system of the animals the sorbent effectively bound and eliminated from the circulation molecules of diphtheric toxin and antitoxin, reduced significantly their levels in the blood after hemoperfusion. The effectiveness of the immunosorbent DATs was confirmed when it saved the ginea-pigs after injection of lethal toxin doses.
