The effects of rumen nitrogen balance on intake, nutrient digestibility, chewing activity, and milk yield and composition in dairy cows vary with dietary protein sources.

The objective was to study the interaction effects of rumen nitrogen balance (RNB) and dietary protein source on feed intake, apparent total-tract digestibility (ATTD), eating and ruminating activity, milk yield (MY), and milk composition in lactating dairy cows. Twenty-four lactating Holstein cows were divided in 4 groups, which were randomly assigned to the dietary treatments included in a replicated 4 × 4 Latin square experimental design that consisted of four 20-d periods, each with 12 d of adaptation to the experimental diets and 8 d of sampling. The dietary treatments followed a 2 × 2 factorial arrangement with 2 main protein sources, faba bean grain (FB) and SoyPass (SP; Beweka Kraftfutterwerk GmbH), offered at 2 dietary RNB levels: RNB0 (RNB of 0 g/kg of dry matter) and RNB- (RNB of -3.2 g/kg of dry matter; i.e., 4 treatments). The composition of concentrate mixtures was adjusted to create diets with the desired RNB levels. Each of the protein sources supplied ≥35% of the total dietary crude protein (CP). Both diets within a protein source had similar forage sources and forage to concentrate ratios and were iso-energetic, but differed in CP concentrations. The main effects of RNB, protein source, and their interactions were tested by PROC MIXED in SAS 9.4 (SAS Institute Inc.). Interaction effects were observed for daily dry matter intake and energy-corrected MY, which were lower for RNB- than RNB0 in diets containing FB (23.5 vs. 24.4 kg dry matter/d; 28.6 vs. 30.6 kg milk/d), but similar in diets containing SP (24.2 vs. 24.3 kg dry matter/d; 31.3 vs. 31.7 kg milk/d). The ATTD of NDF was lower for RNB- compared with RNB0 in the FB (44.9 vs. 49.1 g/100 g) and SP (48.5 vs. 51.9 g/100 g) diets, and greater for the SP than for FB diets. There were interaction effects for ATTD of CP and concentrations of milk urea nitrogen, which were lower for RNB- compared with RNB0 in both, FB (55 vs. 63.1 g/100 g of CP; 5.65 vs. 11.3 mg/dL milk) and SP diets (60 vs. 64.4 g/100 g of CP; 8.74 vs. 13.4 mg/dL milk). However, differences between RNB levels were greater for FB than for SP diets. Furthermore, proportions of milk fatty acids followed the same pattern as that of dietary fatty acids, but biohydrogenation appeared to be greater for RNB- than RNB0 for both protein sources and in FB than in SP diets for both RNB levels. There was an interaction effect on total number of chews per unit of NDF intake, which was greater for RNB- compared with RNB0 for both protein sources. However, the differences between RNB levels were greater in FB than in SP diets. Overall, differences in the animal responses to negative RNB between FB and SP diets suggest a need to better understand the effect of negative RNB levels with different dietary ingredients at similar utilizable CP supply.

The effects of rumen nitrogen balance on nutrient intake, nitrogen partitioning, and microbial protein synthesis in lactating dairy cows offered different dietary protein sources.

The aim was to study the effects of rumen N balance (RNB), dietary protein source, and their interaction on feed intake, N partitioning, and rumen microbial crude protein (MCP) synthesis in lactating dairy cows. Twenty-four lactating Holstein cows were included in a replicated 4 × 4 Latin square experimental design comprising four 20-d periods, each with 12 d of adaptation to the experimental diets and 8 d of sampling. The dietary treatments followed a 2 × 2 factorial arrangement (i.e., 4 treatments) with 2 main protein sources [faba bean grain (FB) and SoyPass (SP; Beweka Kraftfutterwerk GmbH, Heilbronn, Germany)] offered at 2 dietary RNB levels each [0 g/kg of dry matter, DM (RNB0) and -3.2 g/kg of DM (RNB-)]. The RNB was calculated as the difference between dietary crude protein (CP) intake and the rumen outflow of undegraded feed CP and MCP and divided by 6.25. Composition of concentrate mixtures was adjusted to create diets with desired RNB levels. Each of these protein sources supplied ≥35% of total dietary CP. Both diets for each protein source were isoenergetic but differed in CP concentrations. The DM intake (kg/d) was lower for RNB- than for RNB0 in diets containing FB, whereas no differences were seen between the RNB levels for SP diets. The RNB- decreased N intake and urinary N excretion but increased milk N use efficiency in both FB and SP diets, with greater differences between the RNB levels for FB diets than for SP diets. Similarly, duodenal MCP synthesis (g/kg of digestible organic matter intake) estimated from purine derivatives in the urine was lower for RNB- than for RNB0 in FB diets but similar between the RNB levels in diets containing SP. Low RNB of approximately -65 g/d (approximately -3.2 g/kg of DM) in diets reduced feed intake, N balance, and performance in high-yielding dairy cows with possibly more pronounced effects in diets containing rapidly degradable protein sources.