Synthesis and in vitro characteristics of biogenic-derived hydroxyapatite for bone remodeling applications.

The inorganic component of bone matrix, hydroxyapatite (HAp) (with formula Ca10(PO4)6(OH)2), can be obtained from inexpensive waste resources that serve as excellent calcium precursors. In the present study, HAp nano-powder was synthesized from eggshells (ES) and crab shells (CS) by wet chemical precipitation method. Also, a hybrid sample was considered which is a mixture of HAp nano-powder synthesized from eggshells (25%) and crab shells (75%) (EC). The presence of phosphate, carbonate, and hydroxyl groups in the synthesized powder was confirmed through FTIR analysis. The phase composition was determined using XRD, and elemental analysis revealed a Ca/P ratio ranging from 1.5 to 1.8, confirming the HAp nature of the nano-powder, which ranged in size from 73 to 375 nm. Importantly, preliminary in vitro tests were conducted using mouse preosteoblast cell line MC3T3-E1 to evaluate the cytotoxic effects of the synthesized HAp. The results indicated excellent biocompatibility. Moreover, sample EC exhibited a significantly higher proliferation on days 3, 6, 9, and 12. EC demonstrated promising antimicrobial properties by exhibiting a significantly higher inhibitory effect against the bacteria Streptococcus mutans and Escherichia coli, and the fungi Candida albicans and Aspergillus niger. Additionally, EC displayed notable antioxidant activity, with IC50 values of 271.543 µg/ml and 407.764 µg/ml in DPPH and H2O2 assays, respectively. Furthermore, it showed strong anti-inflammatory properties, with a dose-dependent inhibition against protein denaturation. Given these findings, the synthesized HAp holds promise as a potential bone filler and could be beneficial for bone remodeling applications.

The synergic impact of lignin and Lactobacillus plantarum on DSS-induced colitis model via regulating CD44 and miR 199a alliance.

Chronic or recurrent immune system activation and inflammation inside the gastrointestinal tract is characterized by inflammatory bowel disease (IBD). Due to the lack of safety and efficacy of traditional medications, the use of food supplements for IBD management is on the rise. Numerous studies reported that, certain food supplements have a variety of therapeutic benefits for IBD. In the present study, a mouse model of IBD was used to the anti-colitis effects of lignin supplementation with Lactobacillus plantarum (L. plantarum) on intestinal inflammation. The animal model was treated with dextran sodium sulphate (DSS), the illness index increased, and colon length and body weight declined, but these effects were reversed when lignin and L. plantarum treated groups. In addition, lignin and L. plantarum supplementation inhibited the DSS induced increase in levels of cytokines TNF-α (250 pg/mL), INF-γ (180 pg/mL), IL-1ß (70 pg/mL) and TGF- ß (72 pg/mL). Gene and protein expression study revealed that Lignin and L. plantarum supplementation restored the expression of E-cad and suppressed the expression of STAT3 in DSS induced colitis model. Lignin and L. plantarum supplementation also suppressed CD44 expression (1.2 fold) by up regulating the expression of miR199a (1 fold) over DSS induced colitis. Our study suggests that Lactobacillus, lignin, and their synergistic treatments have protective roles against inflammatory bowel disease through changes in inflammatory cytokines, and miR 199a expression in DSS-induced colitis.

Evaluation of probiotic properties of Lysinibacillus macroides under in vitro conditions and culture of Cyprinus carpio on growth parameters.

Cyprinus carpio is an important freshwater fish in aquaculture. It was used for the isolation of potential probiotic strain for aquaculture applications. The most dominant strain was isolated on MRS agar from the gastrointestinal (GI) of C. carpio and identified as Lysinibacillus macroides using molecular marker 16S rRNA gene. Various probiotic properties such as acid and bile tolerance and antibiotic susceptibility were analysed under in vitro conditions. Further, formulate pelletized feed using probiotic (L. macroides) in different concentrations (2, 4, 6 and 8%). Rearing of C. carpio was carried out 45 days and fed with formulated feed. The highest length (5.14 ± 0.07 cm) and weight (3.56 ± 0.07 g) of C. carpio fingerlings was recorded in the 8% LM probiotic pelletized feed, while in fingerlings fed with control showed lower in the length (3.02 ± 0.13 cm) and the weight (0.92 ± 0.04 g) on the 45th day of the experiment. Both percentage of weight gain (PWG) and specific growth rate (SGR) were significantly increased (P < 0.05) of C. carpio fingerlings fed with probiotic feed compared to control feed. Hence, the use of probiotic bacteria could be an encouraging alternative feed for future endeavours in the field of aquaculture. In conclusion, L. macroides can serve as probiotic for sustainable, competitive and promising beneficial bacteria to aquaculture industry.

Pharmacological analysis of hydroethanolic extract of Senna alata (L.) for in vitro free radical scavenging and cytotoxic activities against HepG2 cancer cell line.

The main objective of this study was to evaluate the hydroethanolic extract of Senna alata for the possible free radical scavenging and cytotoxic properties. Using such hydroethanolic extract, various in vitro antioxidant assays at different concentrations were performed and analyzed. In all the assays, plant extract has good inhibitory effect. Ethanolic extract of Senna alata was further subjected into cytotoxicity against HepG2 cell line. Accordingly, it was also found that the plant extract has appreciable potency against cancer cell lines.