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Dev Comp Immunol ; 38(2): 224-31, 2012 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-22469658

RESUMO

Teleost IL-6 is upregulated after antigen stimulation; therefore, we hypothesized that fish IL-6 contributes to antibody production during immune responses against infections. To verify this hypothesis, we first cloned IL-6R and gp130 in fugu (Takifugu rubripes) in the present study. The membrane and soluble forms of IL-6R were identified by the identification of cDNA clones of IL-6R homologues. Three STAT3-docking sites were found in the intracellular region of fugu gp130. Expression analysis showed that fugu IL-6R and gp130 were expressed in mIgM(+) B cells, suggesting that fugu B cells are stimulated by IL-6. Recombinant fugu IL-6 (rfIL-6) increased the gene expression of secretory antibodies by mIgM(+) B cells in vitro. The rfIL-6 and soluble form of rfIL-6R activated STAT3 phosphorylation in the B cells and a cultured cell line transfected with fugu gp130. These results indicate that fugu IL-6 enhances antibody production in the B-cell lineage via gp130 and STAT3 signaling.


Assuntos
Formação de Anticorpos , Receptor gp130 de Citocina/metabolismo , Proteínas de Peixes/metabolismo , Interleucina-6/imunologia , Receptores de Interleucina-6/metabolismo , Fator de Transcrição STAT3/metabolismo , Transdução de Sinais , Takifugu/imunologia , Sequência de Aminoácidos , Animais , Linfócitos B/metabolismo , Clonagem Molecular , Humanos , Dados de Sequência Molecular , Filogenia , Alinhamento de Sequência
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