Generation and characterization of GATA6-specific EGFP expressing human induced pluripotent stem cell line, KSCBi017-A-1, using CRISPR/Cas9.

GATA6 is expressed during early embryogenesis and localizes to endoderm- and mesoderm-derived tissues during later embryogenesis. Here, we established a human induced pluripotent stem cell (hiPSC) line expressing EGFP under GATA6 gene. EGFP coding sequence was introduced into the C-terminus of GATA6 in KSCBi017-A hiPSCs through homologous recombination using CRISPR/Cas9 system. The successfully edited line, KSCBi017-A-1, was selected and confirmed by sequencing. The line had a normal karyotype and exhibited potential to differentiate into three germ layers while it expressed EGFP upon endoderm induction. KSCBi017-A-1 cells can be used to monitor the expression of GATA6 during differentiation. This cell line is available from Korea National Stem Cell Bank.

Changes in energy consumption according to building use type under COVID-19 pandemic in South Korea.

An unprecedented global lockdown has been implemented for controlling the spread of COVID-19 in many countries. These actions are reducing the number of coronics, but with the prolonged COVID-19 outbreak, the restrictions on the activities of people are having a significant impact on all industries. Accordingly, this study aimed to statistically analyze changes in building energy consumption under the COVID-19 pandemic in South Korea, as well as identify the relationship between COVID-19 and building energy consumption according to the building use type. As a result, the average rate of changes in electricity and gas energy consumption decreased -4.46% and -10.35%, respectively, compared to the previous year. The energy consumption in most facilities has tended to decrease while energy consumption in residential facilities increased during COVID-19. The rate of change in building energy consumption had a significantly positive correlation with COVID-19 related factors in various facilities (e.g., neighborhood, religious, educational, and research facilities). Significant findings of this study that social distancing by the COVID-19 outbreak, has changed energy consumption according to building use type indicates the need for new energy systems to effectively manage the energy demand at the community level in the Post COVID-19 era.

Towards environmental sustainability in the local community: Future insights for managing the hazardous pollutants at construction sites.

Although various technologies are being developed in the construction industry, management technologies for achieving environmental sustainability in the local community are still lacking. As such, this study suggests future insights for the development of an automated intelligent environment management system for the promotion of environmental sustainability in the local community, through a systematic review of 1,707 relevant literature. The systematic review was conducted in two steps: (i) quantitative review: keyword co-occurrence and trend analysis; and (ii) qualitative review: a review on monitoring, evaluation, and improvement technologies. As a result, the research level related to the local-level pollutants (noise, vibration, and dust) was found to be quantitatively insufficient, and the limitations of the existing technologies for these pollutants were presented. Eventually, to overcome these limitations, new technologies and application strategies that can be applied to construction sites as future research roadmap to effectively manage the hazardous pollutants were proposed. Furthermore, an intelligent management system should be developed, and the management of environmental complaints is also necessary for environmental sustainability at the local level in the construction industry. As a fundamental study, this study could become a benchmark for future researches dealing with environmental sustainability and hazardous pollutants in the construction industry.

Development of a real-time automated monitoring system for managing the hazardous environmental pollutants at the construction site.

The management of noise, vibration, and dust, which are hazardous pollutants from construction sites, is essential to minimize the health damage of the nearby residents and the economic damage of construction companies due to pollutants from construction sites. For the effective management of hazardous pollutants, their emissions from construction sites must be identified immediately and accurately. Therefore, this study developed a real-time automated monitoring system named "MOnitoring for Noise, Vibration, and Dust (MONVID)" for comprehensively measuring the hazardous environmental pollutants and managing them in real-time. Toward this end, the optimal design of MONVID was planned and customized considering mobility, usability, and economy. Also, for the field application of the developed MONVID, its feasibility was verified by comparing its techno-economic performance with that of the conventional measurement system through experiments. Based on the results of the experiment and performance evaluation, it was concluded that MONVID is a feasible and economical construction pollutant measurement system with reliable technical performance and improved mobility and usability compared to the conventional measurement system. This study has significant contributions to the development of the first platform (including hardware, sensor network, and software) for the integrated real-time automated monitoring of the environmental performance of construction sites.

Incidence and severity of Visually Induced motion Sickness during 3D laparoscopy In Operators who had No experience with it (VISION).

AIM: The aim of the study was to evaluate the incidence and severity of visually induced motion sickness (VIMS) during 3D laparoscopy, in operators without prior experience. MATERIAL AND METHODS: Design: A retrospective comparative study (Canadian Task Force classification II-2). Setting: A university hospital. Intervention: Gynecologic surgery. Main outcome measure: This is a prospective observational study, which enrolled 9 surgeons as participants. None of these surgeons had any prior experience with 3D laparoscopy. Each participant performed 10 consecutive cases of 3D laparoscopy in patients with benign or premalignant gynecological diseases. The primary outcome measure was the incidence and severity of VIMS, which was evaluated using the validated Simulator Sickness Questionnaire. Personal preferences, discomfort, and ease of 3D laparoscopy were also evaluated. RESULTS: Sixty-seven percent of surgeons experienced VIMS during their first 3D laparoscopy case. The incidence and severity of VIMS dramatically decreased from the second case onward. However, in some surgeons (22-44%), VIMS did not completely disappear until the tenth case. With respect to the discomfort using 3D laparoscopy, 84 self-reported responses after each surgery were "favor 3D laparoscopy," and "no" in 61 (72.6%) and 47 (55.9%) participants, respectively. Most participants found it easier to perform 3D laparoscopy than 2D laparoscopy. CONCLUSIONS: The occurrence of visually induced symptoms in susceptible individuals during 3D laparoscopy is high, particularly during their first case. This suggests the need for increasing surgeons' awareness regarding the possibility of discomfort.

Computed terahertz near-field mapping of molecular resonances of lactose stereo-isomer impurities with sub-attomole sensitivity.

Terahertz near-field microscopy (THz-NFM) could locally probe low-energy molecular vibration dynamics below diffraction limits, showing promise to decipher intermolecular interactions of biomolecules and quantum matters with unique THz vibrational fingerprints. However, its realization has been impeded by low spatial and spectral resolutions and lack of theoretical models to quantitatively analyze near-field imaging. Here, we show that THz scattering-type scanning near-field optical microscopy (THz s-SNOM) with a theoretical model can quantitatively measure and image such low-energy molecular interactions, permitting computed spectroscopic near-field mapping of THz molecular resonance spectra. Using crystalline-lactose stereo-isomer (anomer) mixtures (i.e., α-lactose (≥95%, w/w) and ß-lactose (≤4%, w/w)), THz s-SNOM resolved local intermolecular vibrations of both anomers with enhanced spatial and spectral resolutions, yielding strong resonances to decipher conformational fingerprint of the trace ß-anomer impurity. Its estimated sensitivity was ~0.147 attomoles in ~8 × 10-4 µm3 interaction volume. Our THz s-SNOM platform offers a new path for ultrasensitive molecular fingerprinting of complex mixtures of biomolecules or organic crystals with markedly enhanced spatio-spectral resolutions. This could open up significant possibilities of THz technology in many fields, including biology, chemistry and condensed matter physics as well as semiconductor industries where accurate quantitative mappings of trace isomer impurities are critical but still challenging.

Influence of Fatigue, Depression and Anxiety on Quality of Life in Pregnant Women with Preterm Labor.

PURPOSE: The purpose of this study was to identify effects of fatigue, depression and anxiety on quality of life in pregnant women with preterm labor. METHODS: With a survey design, data were collected from 138 mothers who were admitted at a hospital in Seoul, between June 2014 and September 2015. Instruments used to collect the data for the study were: Fatigue Continuum Form, Depression anxiety stress scale (DASS-21) and maternal postpartum quality of life (MAPP-QOL). RESULTS: The mean fatigue score was 68.30 with 50.7% of women being depressed and 79.7% of the 138 women being anxious. The mean quality of life was 18.92 with quality of life being associated with fatigue, depression and anxiety. Depression and fatigue explained 26% of the variance in quality of life. CONCLUSION: Depression and fatigue adversely affected women's quality of life. It is important to address appropriate management of depression and fatigue in order to improve quality of life in pregnant women with preterm labor.