Acetaminophen Disrupts the Development of Pharyngeal Arch-Derived Cartilage and Muscle in Zebrafish.

Acetaminophen is a common analgesic, but its potential effects on early embryonic development are not well understood. Previous studies using zebrafish (Danio rerio) have described the effects of acetaminophen on liver development and physiology, and a few have described gross physiological and morphological defects. Using a high but non-embryonic lethal dose of acetaminophen, we probed for defects in zebrafish craniofacial cartilage development. Strikingly, acetaminophen treatment caused severe craniofacial cartilage defects, primarily affecting both the presence and morphology of pharyngeal arch-derived cartilages of the viscerocranium. Delaying acetaminophen treatment restored developing cartilages in an order correlated with their corresponding pharyngeal arches, suggesting that acetaminophen may target pharyngeal arch development. Craniofacial cartilages are derived from cranial neural crest cells; however, many neural crest cells were still seen along their expected migration paths, and most remaining cartilage precursors expressed the neural crest markers sox9a and sox10, then eventually col2a1 (type II collagen). Therefore, the defects are not primarily due to an early breakdown of neural crest or cartilage differentiation. Instead, apoptosis is increased around the developing pharyngeal arches prior to chondrogenesis, further suggesting that acetaminophen may target pharyngeal arch development. Many craniofacial muscles, which develop in close proximity to the affected cartilages, were also absent in treated larvae. Taken together, these results suggest that high amounts of acetaminophen can disrupt multiple aspects of craniofacial development in zebrafish.

Age Differences in Psychological Antecedents and Behavioral Consequences of Stigmatization Associated with COVID-19 among Koreans.

The first goal of this study is to develop a conceptual model of the causal relationship between psychological antecedents (internal attribution, anger, dangerousness, fear) of stigmatization, stigmatization (public stigma, anticipated stigma), and the behavioral consequences (compliance with COVID-19 prevention guidelines, COVID-19 testing intention) of stigmatization associated with COVID-19. The second goal of the study is to investigate the age differences in the conceptual model between younger and older adults unconfirmed with COVID-19 in Korea. After building the model based on previous studies, an online survey was conducted with Koreans in their 20s (n = 300, females: 50%) and 60s (n = 300, females: 50%) who had not been confirmed with COVID-19. The results revealed that for participants in their 20s and 60s, their internal attribution of COVID-19 infection to individuals confirmed with COVID-19 enhanced their anger at the individuals. Afterward, their anger increased their anticipated stigma of being confirmed with COVID-19 through enhancing the public stigma of the individuals confirmed with COVID-19. Unexpectedly, the fear of individuals confirmed with COVID-19 elicited by the dangerousness of the individuals had no effect on the public stigma of the individuals among participants in their 20s and 60s. The fear directly enhanced their compliance with the COVID-19 prevention guidelines. Next, for participants in their 20s, their anticipated stigma increased their compliance with COVID-19 prevention guidelines, but not their COVID-19 testing intention. However, the anticipated stigma did not affect both the compliance with the COVID-19 prevention guidelines and COVID-19 testing intention among participants in their 60s. The implications and limitations of these findings are discussed.

Age and Cultural Differences in Recognitions of Emotions from Masked Faces among Koreans and Americans.

This study investigates age and cultural differences in the negative effects of senders' wearing masks on receivers' readabilities of senders' facially expressed emotions in interpersonal interactions. An online experiment was thus conducted with Koreans and Americans aged over 20 years. Based on sampling quotas by nationality, age group and gender, Korean (n = 240) and American (n = 273) participants were recruited from panel members of a Korean research company and Amazon's Mechanical Turk via email and the website, respectively. The participants played receiver roles to infer senders' facially expressed emotions presented in photos in the experiment. They judged emotions facially expressed by the senders without masks and with masks are shown in photos. The results revealed that the senders' wearing masks reduced the readabilities of the senders' facially expressed anger among participants aged 30-49 years more than among participants aged 20-29 years. The senders' wearing masks decreased the readabilities of the senders' facially expressed fear for participants in their 50's more than for participants in their 20's. When the senders wore masks, the readabilities of the senders' facially expressed happiness dropped among participants aged over 60 years more than among participants aged 20-49 years. When senders wore masks, American participants' readabilities of disgust, fear, sadness and happiness expressed in the senders' faces declined more than Korean participants' readabilities of those emotions. The implications and limitations of these findings are discussed.

Fabrication of Three-Dimensional Multilayer Structures of Single-Walled Carbon Nanotubes Based on the Plasmonic Carbonization.

We developed a complex three-dimensional (3D) multilayer deposition method for the fabrication of single-walled carbon nanotubes (SWCNTs) using vacuum filtration and plasmonic carbonization without lithography and etching processes. Using this fabrication method, SWCNTs can be stacked to form complex 3D structures that have a large surface area relative to the unit volume compared to the single-plane structure of conventional SWCNTs. We characterized 3D multilayer SWCNT patterns using a surface optical profiler, Raman spectroscopy, sheet resistance, scanning electron microscopy, and contact angle measurements. Additionally, these carbon nanotube (CNT) patterns showed excellent mechanical stability even after elastic bending tests more than 1000 times at a radius of 2 mm.