Simultaneous determination of three Aconitum alkaloids in six herbal medicines by high-performance liquid chromatography.

To simultaneously determine three components of aconitine, mesaconitine, and hypaconitine in six species of Aconitum genus, an extraction condition for the total alkaloids was specifically optimized and a simple analytical method of reversed-phased highperformance liquid chromatography (HPLC) was developed. The extraction rate of total alkaloids in A. szechenyianum Gay was 98.3% for repeated extracting three times with an acidic alcohol solution (alcohol: pH 3.0 HAc = 85:15, v/v). The chromatography was carried out on a Phenomenex Luna C(18) column by gradient elution with a mobile phase of 0.03 mol/mL ammonium bicarbonate (pH = 9.50) -acetonitrile at a flow rate of 1.0 mL/min. The method for all three alkaloids had good linear relationships (r > 0.999) in the concentration range of 1.0-200.0 µg/mL. The average recoveries were 96.6-103.1%, and the LOQ and LOD were in the range of 25-37 ng/mL and 9-12 ng/mL, respectively. The quantitative results indicated that contents of the three alkaloids varied significantly among crude aconite roots, so quality control of traditional Chinese medicines containing aconite roots should be taken into account.

LC-MS analysis for the components captured by ECV304 cell from extract of Aconitum szechenyianum Gay.

A novel method of cell affinity screening (CAS), cell affinity capture coupled with LC-MS analysis, was developed for screening the bioactive compounds related to cardiovascular diseases from the natural product libraries. One of the major characteristics lies in its function in affinity-capturing and separating the bioactive components from the natural product libraries in vitro. Another characteristic is its use in analyzing and identifying the target compounds, by employing high-performance liquid chromatography and mass spectrometry. CAS was used for screening the bioactive components from the alkaloid extract derived from Aconitum szechenyianum Gay. Of the five components found to be bound to the oxidative-damaged endothelial cells, the two compounds identified, mesaconitine and aconitine, were recognized in the literature as being related to cardiovascular diseases.

[Study on the optimum extracting conditions of total alkaloids and aconitine in Aconitum szechenyianum Gay].

OBJECTIVE: To optimize the conditions for extractions of total alkaloids and aconitine from Aconitum szechenyianum Gay. METHODS: Using L16 (4(5)) orthogonal test and univariant methods, the exrtaction conditions of total alkaloids aconitine in Aconitum szechenyianum Gay. were optimized under the guidance of the content determination of total alkaloids with bromophemol blue colorimetry and aconitine with HPLC method, respectively. RESULTS: The obtained optimum condition of total alkaloids was that the material was refluxed in 6 times (m/v) acidic alcohol solution( alcohol: pH 3.0 HAc = 85:15, v/v) for 1 h with 3 times. The condition of aconitine was that the material was refluxed in 4 times (m/v) acidic alcohol solution (alcohol: pH 3. p HAc = 15:85, v/v) for 0.5 h with 3 times. Contents of total alkaloids and aconitine in Aconitum szechenyianum Gay. were 0.980% and 0.109%, respectively. CONCLUSION: Through the test and verify, the optimum extraction methods of total alkaloids and aconitine are rational.