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1.
RSC Adv ; 9(42): 24241-24247, 2019 Aug 02.
Artigo em Inglês | MEDLINE | ID: mdl-35527906

RESUMO

Zwitterionic hydrogels have promising potential as a result of their anti-fouling and biocompatible properties, but they have recently also gained further attention due to their controllable stimuli responses. We successfully synthesized two zwitterionic polymers, poly(2-methacryloyloxyethyl phosphorylcholine) (poly-MPC) and poly(2-(methacryloyloxy)ethyl dimethyl-(3-sulfopropyl)ammonium hydroxide) (poly-DMAPS), which have complementary ionic sequences in their respective zwitterionic side groups and likely form an interpenetrating double network to improve their mechanical strength. The synthesized poly-MPC was blended in a poly-DMAPS matrix (MD gel) and showed high viscosity, while poly-DMAPS was blended in a poly-MPC hydrogel (DM gel) and revealed UCST behavior as the temperature increased. In addition, cross-section images of the MD hydrogel exhibited its compact and uniform structure, while the DM gel was found to exhibit a porous micro-structure with clear boundaries. The results explained the low viscosity of the DM gel, which was also confirmed via 3D Raman mapping. To sum up, the preliminary data demonstrated that binary zwitterionic hydrogels have thermosensitive mechanical properties, promoting further bio-applications in the future, such as in wound healing.

2.
Adv Sci (Weinh) ; 5(2): 1700339, 2018 02.
Artigo em Inglês | MEDLINE | ID: mdl-29610720

RESUMO

Combination of chemotherapy and radiotherapy is used to enhance local drug delivery while reducing off-target tissue effects. Anticancer drug doxorubicin (DOX) is loaded into l-cysteine modified G4.5 dendrimer (GC/DOX) and released at different pH values in the presence and absence of γ-radiation. Presence of γ-radiation significantly improves DOX release from the GC/DOX under acidic pH conditions, suggesting that GC dendrimer is a radiation-sensitive drug delivery system. GC/DOX is further evaluated by determining cytotoxicity in uterine cervical carcinoma HeLa cells. GC/DOX shows high affinity for cancer cells and effective drug release following an external stimulus (radiation exposure), whereas an in vivo zebrafish study confirms that l-cysteine acts as a radiosensitizer. GC/DOX treatment combined with radiotherapy synergistically and successfully inhibits cancer cell growth.

3.
Int J Mol Sci ; 18(5)2017 May 19.
Artigo em Inglês | MEDLINE | ID: mdl-28534813

RESUMO

Here, dual-sensitive fluorescent branched alginate-polyethyleneimine copolymer (bAPSC) nanogels were synthesized from thiolated alginate and stearoyl-derivatized branched polyethyleneimine. The formation of bAPSC conjugates was confirmed through proton nuclear magnetic resonance and Fourier transform infrared spectroscopy, whereas dynamic light scattering was used to measure the particle size and ζ potential of the nanogels. The fluorescent properties of the nanogels were confirmed through fluorescent spectroscopy and microscopy. In addition to the excitation-dependent fluorescence behavior, the fluorescence emission intensity of bAPSC was altered by both pH and γ-irradiation. This intensity was higher at a lower pH than at a higher pH, and it slightly decreased after γ-irradiation. The drug loading and encapsulation efficiency of bAPSC were 25.9% and 11.2%, respectively. An in vitro drug release study revealed that the synthesized nanogels release their doxorubicin (Dox) contents in a time-dependent manner, and the drug release was higher after 96 h of incubation. Approximately 43.74% and 88.36% of Dox was released after 96 h of incubation at pH 5.5 in the absence and presence of glutathione (GSH), respectively. However, relatively lower drug release, approximately 21.6% and 16%, was observed in the presence and absence of GSH at pH 7.4, respectively. Fluorescence microscopy confirmed that Dox-loaded bAPSC nanogels were internalized by HeLa cells, and drug distribution was easily tracked using fluorescent materials without additional probing agents. Moreover, cellular cytotoxicity and hemolysis results revealed less cytotoxicity and hemocompatibility of the synthesized nanogels, confirming that they are the most favorable alternative drug carriers for drug delivery systems.


Assuntos
Alginatos/química , Antibióticos Antineoplásicos/administração & dosagem , Preparações de Ação Retardada/química , Doxorrubicina/administração & dosagem , Corantes Fluorescentes/química , Polietilenoimina/química , Antibióticos Antineoplásicos/farmacocinética , Doxorrubicina/farmacocinética , Sistemas de Liberação de Medicamentos , Liberação Controlada de Fármacos , Géis/química , Ácido Glucurônico/química , Glutationa/química , Células HeLa , Ácidos Hexurônicos/química , Humanos , Concentração de Íons de Hidrogênio , Microscopia de Fluorescência , Nanopartículas/química , Oxirredução , Tamanho da Partícula
4.
Nanotechnology ; 22(18): 185303, 2011 May 06.
Artigo em Inglês | MEDLINE | ID: mdl-21415468

RESUMO

In this study, we have used an agarose stamp to conduct direct printing of silver nanoparticles, nanowires and nanoplates on both planar and structured substrates. Nanoparticle solution could be first coated on an agarose stamp, and then transferred to a planar substrate. Micro-patterns comprising metal nanoparticles could be printed on planar substrates without the formation of residual layers. Thus a three-dimensional metal microstructure could be easily fabricated. The patterning of electrodes by printing Ag nanowires directly on TiO(2) was also demonstrated to fabricate resistive random access memory (RRAM) devices by all-solution-processing methods. By using a flat agarose stamp, the patterns printed on the microstructured substrates were quite different from those on the nanostructured substrates. On the microstructured substrates, direct printing could print silver nanoparticles onto the protrusion surface, and could print silver layers as thick as several microns, useful for high conductivity electrodes. On the substrates with nanostructures such as photonic crystals or nano-gratings, direct printing could transfer nanoparticles into the grooves or cavities only due to the contact of the agarose stamp with the groove or concavity surface. A new approach to fabricate metal wire grid polarizers was further demonstrated. A nanoporous agarose stamp has a good potential for printing using nanoparticle suspension.

5.
Macromol Biosci ; 5(3): 246-53, 2005 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-15768444

RESUMO

Nanophase separation has been suggested to influence the biological performance of polyurethane. In a previous work, six different 4,4'-diphenylmethane diisocyanate (MDI)-based poly(carbonate urethane)s (PCUs) that exhibited various degrees of nanophase separation were synthesized and characterized. In the present work, these PCUs were used as a model system to study the effect of nanometric structures on the biocompatibility of polyurethane. Human blood platelet activation, monocyte activation, protein adsorption, and bacterial adhesion on PCU were investigated in vitro. It was found that human blood platelets as well as monocytes were less activated on the PCU surfaces with a greater degree of nanophase separation in general. This phenomenon was closely associated with the lower ratio of human fibrinogen/albumin competitively adsorbed on these surfaces. Bacterial adhesion was also inhibited in some nanophase-separated PCUs. [diagram in text].


Assuntos
Materiais Biocompatíveis/farmacologia , Fenômenos Fisiológicos Sanguíneos/efeitos dos fármacos , Poliuretanos/farmacologia , Bacillus subtilis/efeitos dos fármacos , Materiais Biocompatíveis/isolamento & purificação , Células Sanguíneas/efeitos dos fármacos , Humanos , Nanotecnologia , Poliuretanos/isolamento & purificação
6.
Macromol Biosci ; 4(4): 464-70, 2004 Apr 19.
Artigo em Inglês | MEDLINE | ID: mdl-15468239

RESUMO

In this work, we synthesized two MDI-based polyurethanes, including a poly(ether)urethane (PEU) and a poly(carbonate)urethane (PCU), by using different soft segments, poly(tetramethylene oxide) and poly(hexyl, ethyl)carbonate diol (M approximately 2,000). We demonstrated that, in addition to the enhanced biostability of PCU over PEU, the biological performances of PCU in vitro were also improved in general. These included, better cellular attachment and proliferation, less platelet activation, as well as reduced monocyte activation. The unusual wide-ranging enhancement in biocompatibility for PCU was believed to be related to the larger micro-phase separation in PCU (approximately 25 nm) that caused distinct protein adsorption on the surface. The total number of adherent monocytes (nonactivated and activated) on the bare sample surfaces, albumin pre-adsorbed sample surfaces, and fibrinogen pre-adsorbed sample surfaces.


Assuntos
Materiais Biocompatíveis/química , Polímeros/química , Poliuretanos/química , Adsorção , Materiais Biocompatíveis/síntese química , Fibrinogênio , Teste de Materiais , Microscopia de Força Atômica , Microscopia Eletrônica de Varredura , Peso Molecular , Polímeros/síntese química , Poliuretanos/síntese química , Albumina Sérica , Propriedades de Superfície , Termodinâmica
7.
Macromol Biosci ; 4(9): 891-900, 2004 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-15468298

RESUMO

In this work, we synthesized six 4,4'-diphenylmethane diisocyanate (MDI)-based poly(carbonate urethanes) (PCU) by using the macrodiol poly(1,6-hexyl, 1,2-ethyl carbonate) diol (MW = 2,017) in different molar ratios to MDI. The bulk and surface properties of cast PCU films were analyzed. The glass transition temperatures measured by dynamic mechanical analysis (DMA) and surface images obtained from atomic force microscopy (AFM) indicated that these PCU had various degrees of nanophase separation that changed with the time and film casting temperatures. The degree of nanophase separation correlated very well with endothelial cell attachment and proliferation on PCU.


Assuntos
Polímeros/química , Poliuretanos/química , Materiais Biocompatíveis/química , Adesão Celular , Divisão Celular , Células Cultivadas , Endotélio Vascular/citologia , Vidro , Humanos , Luz , Teste de Materiais , Nanotecnologia , Estresse Mecânico , Temperatura , Fatores de Tempo , Veias Umbilicais/citologia , Água/química
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