Assuntos
Angiografia Coronária/estatística & dados numéricos , Medicare , Infarto do Miocárdio/diagnóstico por imagem , Idoso , Canadá/epidemiologia , Planos de Pagamento por Serviço Prestado/estatística & dados numéricos , Guias como Assunto , Humanos , Programas de Assistência Gerenciada/estatística & dados numéricos , Infarto do Miocárdio/classificação , Infarto do Miocárdio/mortalidade , Estados Unidos/epidemiologia , Revisão da Utilização de Recursos de SaúdeRESUMO
We have determined the identity of the orphan G-protein coupled receptor cDNA, 6H1, present in activated chicken T cells, as a subtype of P2Y purinoceptor. This identification is based on first on the degree of sequence identity shared with recently cloned members of the P2Y receptor family and second on the pharmacological profile. Upon transient expression in COS-7 cells the 6H1 receptor bound the radiolabel [35S]dATP alpha S specifically and with high affinity (Kd, 10 nM). This specific binding could be competitively displaced by a range of ligands active at P2 purinoceptors, with ATP being the most active (K (i)), 116 nM). Such competition studies have established the following rank order of activity: ATP ADP 2-methylthioATP alpha, beta-methylene ATP, UTP, thus confirming 6H1 as a member of the growing family of P2Y purinoceptors. As the fifth receptor of this type to be identified we suggest that it be named P2Y5.
Assuntos
Receptores de Superfície Celular/metabolismo , Receptores Acoplados a Proteínas G , Receptores Purinérgicos P2/metabolismo , Sequência de Aminoácidos , Animais , Ligação Competitiva , Linhagem Celular , Membrana Celular/metabolismo , Galinhas , Chlorocebus aethiops , Sequência Conservada , Nucleotídeos de Desoxiadenina/metabolismo , Humanos , Cinética , Ativação Linfocitária , Dados de Sequência Molecular , Filogenia , Ensaio Radioligante , Receptores de Superfície Celular/biossíntese , Receptores de Superfície Celular/genética , Receptores Purinérgicos P2/biossíntese , Receptores Purinérgicos P2/genética , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/metabolismo , Homologia de Sequência de Aminoácidos , Radioisótopos de Enxofre , Linfócitos T/imunologia , Linfócitos T/metabolismo , Tionucleotídeos/metabolismo , TransfecçãoRESUMO
Alcohol, cocaine, and marijuana use during pregnancy was reported antenatally and at 13 months postpartum by 361 black inner city mothers. The two reports were moderately related for all three substances, but levels reported retrospectively were substantially higher. MAST scores did not differ for the two interviews. Most of the correlations of the antenatal and retrospective reports with maternal and infant characteristics were similar in magnitude; those that differed were somewhat stronger for the antenatal measures. Although the retrospective reports may provide a better indication of mean levels of fetal exposure, they may be less precise in rank ordering among individuals for purposes of correlational analysis. Women with higher MAST scores were particularly prone to report higher levels of both alcohol and cocaine when interviewed retrospectively, and more severely depressed mothers were more likely to report higher levels retrospectively for all three substances. These data suggest that women reporting more than 1.3 drinks/week antenatally (AA/day greater than 0.1) may actually be drinking at levels at risk for alcohol-related birth defects.
