Transcriptomic analysis of salt stress induced chlorophyll biosynthesis-related genes in photoheterotrophic Arabidopsis thaliana calli.

In order to investigate the salt stress induced chlorophyll biosynthesis-related genes in photoheterotrophic cultures, we performed RNA-Seq analysis on A. thaliana calli exposed to 100 mM NaCl on MS medium containing 0.5 mg/L 2,4-D 30 days. Four different conditions of samples were sequenced on Illumina HiSeq Platform in total and generated about 4.49 Gb per sample. The average genome and gene mapping rates were 93.52% and 90.78%, respectively. According to expression profile analysis, some DEGs demonstrated altered related to chlorophyll pigment metabolism. According to analysis, green callus color of photoheterotrophic calli were mainly connected with the induction of LHCB4.3 light harvesting complex photosystem II (Gene ID:818599), AT1G49975 photosystem I reaction center subunit N (Gene ID: 841421), PAM68 PAM68-like protein (DUF3464) (Gene ID: 2745715) and AT3G63540 thylakoid lumenal protein (Mog1/PsbP/DUF1795-like photosystem II reaction center PsbP family protein)(Gene ID: 7922413) genes. Furthermore, 8 DEGs were randomly selected to validate the transcriptome profiles via qPCR. These results will provide a foundation for further studies aimed at giving photosynthetic properties to in vitro plant cultures.

Molecular Effects of Silicon on Arabidopsis thaliana Seedlings under UV-B Stress.

Silicon-plant interaction studies have shown that silicon reduces the harmful effects of stress in plants. Ultraviolet-B (UV-B) radiation, one of the abiotic stress affecting plants, poses a severe problem due to global warming. In this context, it is crucial to examine silicon's effects on UV-B radiation stress at the molecular level. The experiments were carried out on 17 days old Arabidopsis seedlings that were treated with 800 µWatt cm-2 doses of UV-B for 60 min and harvested on the 28th day. 1 mM orthosilicic acid was applied to the in vitro plant tissue culture for experimental groups. According to the results of the osmolyte accumulation analyses, silicon has been shown to play a role in the osmotic stress response. Gene expression levels of DGK2, CHS, FLC, RAD51, and UVR8 were measured via qPCR, and it has been shown that silicon interacts with these genes under UV-B radiation stress. The result of genomic DNA methylation analysis demonstrated that silicon might affect DNA methylation levels by increasing the 5-mC percentage compared with the control group. This study focused on the molecular effects of silicon application. It supports silicon-plant interaction research by demonstrating that silicon might affect UV-B response at the molecular level.