[Microdensitometric research on DNA in the cells of a primary kidney tumor after Spongostan embolization of the renal artery]. / Mikrodensitometrische Untersuchungen der DNS in den Zellen des primären Nierentumors nach der Embolisation der Nierenarterie mit Spongostan.

In klatsch preparations of renal tumours (light-cellular carcinomas with malignity degree 2 and 3) microdensitometric investigations of the DNA-content were performed. In the 1st group the material came from 10 patients in whom a unique nephrectomy, in the 2nd group from 10 patients in whom 2 days before nephrectomy an embolisation of the renal artery with Spongostan, in the 3rd group from 10 patients in whom 14 days after embolisation the nephrectomy had been performed. From the analysis of the histograms follows that in the cytoblasts of the tumours of the 3rd group the percentage of diploid and paradiploid nuclei is higher than in the groups I and II: deviation to the left of the histogram. However, the index of the mean DNA-content is smaller in the 3rd group than in the 1st and 2nd group, correspondingly: 8.79, 10.52 and 11.8; the difference is statistically significant. The results obtained show that the embolisation of the renal artery may not only lead to a necrosis of the neoplasmatic tissue, but also to an inhibition of the DNA-synthesis in the persisting cancer cells.

[Effect of embolization of the renal artery on histologic changes in primary kidney cancer]. / Der Einfluss der Embolisierung der Nierenarterie auf die histologischen Veränderungen beim primären Nierenkrebs.

The authors compared the histologic changes in 12 kidneys removed due to renal carcinoma with the changes in 12 other kidneys in which before nephrectomy an embolization of the renal artery with Spongostan had been performed. The results achieved show that the embolization of the renal artery may intensify the lymphocytic macrophagal and fibroplastic interstice of the tumour and according to this may represent a factor which is able to activate the cellular effector mechanism of the antineoplastic immunity of the organism.

Histological and histochemical changes in the dog kidney after renal artery embolization with Spongostan.

Histological and histochemical examinations of canine kidneys 24 hours, 7, 14 and 24 days after embolization of the renal artery with Spongostan showed that during 24 days the resorption and organization of the infarcts in the embolized kidneys and the resorption of Spongostan in the arteries were not yet completed. The intensified reactions to phosphatases, dehydrogenases, and non-specific esterase indicate a prolonged activation of cells playing a role in the resorption and organization of infarcts which may be of significance in the stimulation of immune responses. There were no inflammatory reactions in the embolized arteries.

Morphometric and histological examinations of dog kidneys after embolization of the renal artery with the cyanoacrylic glue "Chirurcoll-Polfa".

The renal artery in 20 mongrel dogs was embolized with cyanoacrylic glue "Chirurcoll-Polfa", introduced through a catheter. The extent of the resulting renal infarction of the kidneys was evaluated morphometrically and was found to be 71.35 to 91.43% of the volume of the embolized kidneys (mean 77.61%). Histological examinations revealed no resorption of the glue after 3 months. In the embolized vessels, after 7 days tumefaction and degeneration of the reticulin, elastic, and collagen fibres were observed, after 24 days inflammatory changes, and after 2 and 3 months respectively, secondary collagenization and hyalinization. Inflammatory changes were also found in the perivascular tissue. In the sections of the kidneys not involved in the infarction a progressive atrophy of the nephrons and fibrosis of the parenchyma were observed 2 and 3 months after embolization.

Morphometric investigations of dog kidney infarcts after embolization of the renal artery with Spongostan.

A macroscopic morphometric method for evaluating the volume of infarcts in the kidneys of dogs 24 hours, 7, 14, and 24 days after renal artery embolization by means of Spongostan is described. The whole kidney was not infarcted, the volume of the infarcts being 60.15-81.87% (mean 71.1%) of the volume of the embolized kidney. In order to evaluate the distant morphological changes in the parts of the embolized kidney not involved in the infarcts, it would be necessary to carry out microscopic examinations and to have a longer observation period.