RESUMO
Brassinosteroid-induced phosphorylation of tyrosine residues in proteins was studied. Proteins of crude extract of pea leaves were analyzed by one- and two-dimensional electrophoresis followed by Western blotting with monoclonal antibodies PY20 to phosphotyrosine proteins. One- and two-dimensional electrophoresis revealed 7 and 13 tyrosine-phosphorylated proteins, respectively. Brassinolide increased the phosphorylation level of most of these proteins. With inhibitors of tyrosine protein phosphatases, such as phenylarsine oxide and orthovanadate, the level of tyrosine phosphorylation of these proteins increased.
Assuntos
Colestanóis/farmacologia , Pisum sativum/metabolismo , Reguladores de Crescimento de Plantas/farmacologia , Proteínas de Plantas/metabolismo , Esteroides Heterocíclicos/farmacologia , Tirosina/metabolismo , Brassinosteroides , Colestanóis/metabolismo , Eletroforese em Gel Bidimensional , Peso Molecular , Pisum sativum/citologia , Fosforilação , Reguladores de Crescimento de Plantas/metabolismo , Folhas de Planta/citologia , Folhas de Planta/efeitos dos fármacos , Folhas de Planta/metabolismo , Esteroides Heterocíclicos/metabolismoAssuntos
Lipoxigenase/metabolismo , Reguladores de Crescimento de Plantas/farmacologia , Proteínas de Plantas/metabolismo , Transdução de Sinais , Esteroides Heterocíclicos/farmacologia , Colestadienóis/farmacologia , Ácidos Linoleicos/química , Pisum sativum/química , Fosfolipases A/antagonistas & inibidores , Fosforilação/efeitos dos fármacos , FitosteróisRESUMO
We have studied the effect of Mannich base acetate (MBA), an antioxidant, on growth of the yeast, as well as on the level of cAMP and Ca2+ uptake by them. We demonstrate that the antioxidant induced an increase in the level of cAMP in yeast cells and a decrease in Ca2+ uptake by the cells. The effect of the antioxidant depends on the level of Ca2+ in the growth medium.
Assuntos
Antioxidantes/farmacologia , Cálcio/metabolismo , AMP Cíclico/metabolismo , Bases de Mannich/farmacologia , Saccharomyces cerevisiae/efeitos dos fármacos , Saccharomyces cerevisiae/crescimento & desenvolvimento , Saccharomyces cerevisiae/metabolismoRESUMO
The products of the lipoxygenase pathway, methyl jasmonic acid (MeJA) and (9Z)-12-hydroxy-9-dodecenoic acid (HDA), hardly changed the relative level of phosphorylated polypeptides (RLPPs) during 2 h of incubation: 15 and 17 kDa RLPPs were enhanced by HDA, but decreased by MeJA. RLPPs of 73 and 82 kDa were increased by both compounds. MeJA and HDA treatment induced specific and unspecific effects in some RLPPs. It was shown that HDA and MeJA increased protein kinase activity in the presence of 1 microM cAMP.
Assuntos
Acetatos/farmacologia , Ciclopentanos/farmacologia , Ácidos Graxos Monoinsaturados/farmacologia , Fosfatos/metabolismo , Pisum sativum/metabolismo , Reguladores de Crescimento de Plantas/farmacologia , Proteínas de Plantas/metabolismo , Oxilipinas , Pisum sativum/efeitos dos fármacos , Fosfopeptídeos/metabolismo , Fosforilação , Folhas de Planta/metabolismoAssuntos
ATPases Transportadoras de Cálcio/metabolismo , Raios gama , Pirimidinas/farmacologia , Protetores contra Radiação/farmacologia , Linfócitos T/efeitos dos fármacos , Linfócitos T/efeitos da radiação , Animais , DNA Topoisomerases Tipo I/metabolismo , Feminino , Hipersensibilidade Tardia/imunologia , Hipersensibilidade Tardia/prevenção & controle , Técnicas In Vitro , Camundongos , Linfócitos T/enzimologia , Linfócitos T/imunologiaAssuntos
Inibidores de Adenilil Ciclases , Linfócitos B/efeitos dos fármacos , Inibidores Enzimáticos/farmacologia , Pirimidinas/farmacologia , Sulfonas/farmacologia , Linfócitos T/efeitos dos fármacos , Uracila/análogos & derivados , Adenilil Ciclases/sangue , Animais , Linfócitos B/enzimologia , Linfócitos B/imunologia , Combinação de Medicamentos , Cobaias , Humanos , Técnicas In Vitro , Linfonodos/citologia , Linfonodos/efeitos dos fármacos , Linfonodos/enzimologia , Linfonodos/imunologia , Especificidade de Órgãos , Especificidade da Espécie , Linfócitos T/enzimologia , Linfócitos T/imunologia , Timo/citologia , Timo/efeitos dos fármacos , Timo/enzimologia , Timo/imunologia , Uracila/farmacologiaRESUMO
It was demonstrated in experiments on guinea pigs that a 30 mg/kg dose of ximedon changes the content of SH-groups in immunocompetent cells depending on the duration of drug administration and the organ to which the cells under test belong. Maximum increase of the SH-groups was recorded in the thymocytes. The dynamics of changes in Ca(2+)-ATPase activity repeated the fluctuations in the SH-group content in the splenic and bone marrow cells. In the bone marrow cells of CBA x C57B1 mice 3 mg/kg ximedon suppressed the induction of chromosome aberrations caused by cyclophosphane. The interrelationship of changes in the cell sulfhydryl status with Ca(2+)-ATPase activity and the antimutagenic effect of ximedon is discussed.
Assuntos
Antimutagênicos/farmacologia , ATPases Transportadoras de Cálcio/efeitos dos fármacos , Imunocompetência/efeitos dos fármacos , Pirimidinas/farmacologia , Compostos de Sulfidrila/metabolismo , Animais , Células da Medula Óssea/efeitos dos fármacos , Células da Medula Óssea/imunologia , Células da Medula Óssea/metabolismo , ATPases Transportadoras de Cálcio/metabolismo , Aberrações Cromossômicas , Cobaias , Linfonodos/citologia , Linfonodos/efeitos dos fármacos , Linfonodos/imunologia , Linfonodos/metabolismo , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos CBA , Baço/citologia , Baço/efeitos dos fármacos , Baço/imunologia , Baço/metabolismo , Linfócitos T/efeitos dos fármacos , Linfócitos T/imunologia , Linfócitos T/metabolismo , Fatores de TempoRESUMO
Experiments with guinea pig immunocompetent cells and nonadherent and adherent mononuclear fractions of the peripheral blood of healthy donors, and patients with rheumatoid arthritis and purulent surgical infection disclosed different levels of Ca(2+)-ATPase activity. Thymectomy in adult guinea pigs led to diminution of activity of the enzyme in all lymphoid organs. The effect of ximedon (30 mg/kg) on Ca(2+)-ATPase activity depended on the thymus and duration of treatment with the drug, and was of a two-phase character.