RESUMO
A sample of patient's faeces containing virus-like particles (VLP) of 27-34 nm was obtained during an outbreak of hepatitis E in the Kirghiz SSR. The identity of the VLP to hepatitis E virus and etiological association with the disease were demonstrated by immune electron microscopy and infection of Macaca fascicularis monkeys. This isolate of hepatitis E virus is able to induce experimental infection in domestic piglets which was very similar to experimental hepatitis E in primates when infected orally, intravenously and by the combined routes. The clinical manifestations included acute biochemical and histological hepatitis, excretion of hepatitis E virus in faeces, icteric sclerae and skin, hepatitis virus presence in the material from mesenteric lymph nodes. Immunosuppression aggravated hepatitis E infection in piglets. Piglet-to-piglet transmission of hepatitis E virus was demonstrated. During passages of the virus in piglets a shortening of the incubation period and the absence of jaundice was observed.
Assuntos
Hepatite Viral Animal/etiologia , Hepatite Viral Humana/microbiologia , Doenças dos Suínos/etiologia , Animais , Fezes/microbiologia , Anticorpos Anti-Hepatite/sangue , Vírus de Hepatite/imunologia , Vírus de Hepatite/isolamento & purificação , Vírus de Hepatite/patogenicidade , Hepatite Viral Animal/imunologia , Hepatite Viral Animal/microbiologia , Hepatite Viral Animal/patologia , Humanos , Fígado/patologia , Macaca fascicularis , Suínos , Doenças dos Suínos/imunologia , Doenças dos Suínos/microbiologia , Doenças dos Suínos/patologiaRESUMO
It was shown, that in castrated rats dianabol (10 mg/kg of body mass daily for 7 days) inhibits the protein synthesis in thymus. The binding analysis of 3H-dianabol showed the specific binding sites in thymic cytosol, which are identical to those for androgens. Isolated thymocytes didn't bind dianabol.
