Systemic Amyloidosis Model on Young Mice.

Subcutaneous daily injection (with neglect of aseptics) of 0.5 ml solution of soybean cream substitute (10% volume in distilled water) during 30 days caused systemic amyloidosis in 30-day-old mice. All the known methods for induction of systemic amyloidosis are based on the use of old animals, as senile tissue bradytrophy allows effective simulation of amyloidosis.

[The alcohol effect on natural chronobiological rhythms of suicidal activity in patients supervised by addiction treatment specialists and psychiatrists]. / Vliyanie priema alkogolya na khronobiologicheskie ritmy suitsidal'noi aktivnosti u patsientov, nakhodivshikhsya pod nablyudeniem narkologa i psikhiatra.

AIM: To study the alcohol effect on chronological rhythms of suicidal activity in patients supervised by addiction treatment specialists and psychiatrists. MATERIAL AND METHODS: We have analyzed 1094 cases of suicides committed by men who lived in Cheboksary and Novocheboksarsk. The suicide committers were divided into two groups by presence or absence of blood and/or urine alcohol; each group was subdivided into three subgroups: a) mentally healthy suicide committers (MHSC), b) those who were under the supervision of the psychiatrist (PS), c) those who were supervised by addiction treatment specialists (ATS). Chronobiological rhythms were assessed using z-scores, frequency biological rhythms were evaluated with Kolmogorov-Smirnov's test with the Lilliefors correction and rank correlation. The trends of each chronobiological number were computed, the differences of obliquing angle of the trend were evaluated using 2×2 contingency tables (c2 with Mac-Namara's amendment). RESULTS: All suicide committers of the 1st group showed daily rising suicide trend, which had highs in the active wakefulness period. In the 2nd group, the trend did not change in MHSC and ATS, but in PS it significantly changed to descending with two peaks at 6-8:59 and 18-20:59. Upward weekly trend in PS in both groups was almost the same (in the 1st group the obliquing angle of the trend was 19° and in the 2nd group - 21°), the maximum was observed on Saturday and Sunday. In MHSC of the 1st group, the trend was moving downward, and in the 2nd group it was low. Both MHSC and ATS had the maximum on Monday. Annual trend in the 1st group in all samples was low. In the 2nd group, the trend remained low at MHSC and ATS and changed up in PS (16°) with the maximum in November-December. CONCLUSION: Chronobiological characteristics of deaths confirmed by peaks exceeding the average proportion of suicides from 1.5 to 2.5 times were noted in all groups of suicide committers. Alcohol intake significantly changed the suicidal chronobiological rhythm in PS.

[The influence of alcohol consumption on the chronological patterns of sudden cardiac death].

The objective of the present study was the retrospective analysis of the materials collected by the Republican Bureau of Forensic Medical Expertise, Ministry of Health and Social Development of Chuvash Republic, during the period from 1997 till 2002 for the elucidation of the chronological patterns of sudden cardiac death (SCD) associated with alcohol consumption depending on the sex and age of the victims, days of the week and months of the year as well as weather conditions. It was shown that the peak of mortality among men and women taking no alcohol fell on Monday. It was highest in May and decreased by February. The alcohol consumption significantly changes the chronological patterns of mortality from cardiovascular pathology. It was highest among the women abusing alcohol in August and October. The study has demonstrated a weak negative correlation between the frequency of sudden cardiac death among non-consumers of alcohol and dew-point temperature among the persons having alcohol in blood.

Response of glandular organs to experimental macroglossia.

Response of glandular organs to tongue enlargement was studied in 16 outbred male rats; each of them received injections of hydrophobic polyacrylamide gel (0.05 ml) in midline of the tongue. Changes in the studied glandular organs of external secretion (salivary glands) and internal secretion (thyroid and adrenal glands) were morphometrically detected.

[Morpho-functional changes of splenic autotransplants at the different time intervals after the operation]. / Morfofunktsional'nye izmeneniia autotransplantov selezenki v raznye sroki poske operatsii.

This investigation was aimed at the study of splenic autotransplant cytoarchitectonics and cellular structures functional activity 3-60 days after the operation. The studies were carried out in 70 albino male rats including intact animals (n-10) and rats subjected to splenectomy and subsequent spleen heterotopic autotransplantation (n-60). Cryostat sections of transplants were processed for Falc and Cross luminescent-histochemical methods and were stained with hematoxylin and eosin. Biogenic amines content was measured using LUMAM-4 microscope with FMEL-1A spectrofluorimetric device. The number of luminescent cells in periarterial T-dependent zone was found to increase significantly after 7 days, while the unchanged lymphoid nodules were found in the zone of unaltered tissues. At day 15 "intrafollicular" luminescent granulocytes appeared in the newly formed lymphoid nodules. At days 15 to 45 the increased content of biogenic amines was detected in the "intrafollicular" cells, while the significant rise in the amount of amines was seen in the lymphocytes of nodule T-independent zone. It is suggested that the presence of intact nodules in transplant peripheral zone together with an increase in cell numbers in T-dependent zone, are the key events in the development of immune-correction effect of the transplanted spleen during the first two weeks after the operation.

[The effect of reaferon on the level of catecholamines and mitochondrial enzymes in the ischemic kidney]. / Vliianie reaferona na uroven' katekholaminov i fermentov mitokhondrii v ishemicheskoi pochke.

The effect of reaferon (introduced at a dose of 1 x 10(6) IU/kg over six days prior to ischemia induction) on the levels of catecholamines and the activity of succinate dehydrogenase and NADH-dehydrogenase in various structures of kidney was studied in experiments on white rats. The ischemia was modeled by 90-min ligation of renal vessels. Reaferon retained the luminescence of catecholamines in all renal structures 24 h after circulation was restored on the level of intact kidney, except for the nerve trunks where the luminescence intensity decreased by 40%. Preliminary introduction of reaferon stimulated restoration of the enzymatic activity in the Krebs cycle and mitochondrial respiratory chain after 24- and 48-h revascularization, respectively.