RESUMO
Porous blocks of carbonate apatite (CA) were prepared by holding together CA particles ranging in size from 300 to 500 microm through sintering at 750 degrees C for 2 hours. Bone marrow cells taken from Fischer rats were seeded onto and inside the CA blocks and cultured for 14 days to allow stem cells to proliferate to osteoblasts capable of inducing bone formation. Hybrids made of CA blocks and cultured bone marrow cells were then implanted into the back of syngeneic rats. Microfocus x-ray computed tomographic images of tissues containing CA blocks before decalcification suggested that new bone was formed in this extraosseous site 4 and 8 weeks after implantation. These data indicate that the hybrid made of CA and bone marrow cells is capable of inducing heterotopic bone formation in vivo.
Assuntos
Apatitas/química , Materiais Biocompatíveis/química , Células da Medula Óssea/citologia , Engenharia Tecidual , Alicerces Teciduais/química , Animais , Células da Medula Óssea/fisiologia , Cálcio/análise , Adesão Celular , Técnicas de Cultura de Células , Microanálise por Sonda Eletrônica , Processamento de Imagem Assistida por Computador , Masculino , Microscopia Eletrônica de Varredura , Músculo Esquelético/cirurgia , Nitrogênio/análise , Osteogênese/fisiologia , Fósforo/análise , Porosidade , Ratos , Ratos Endogâmicos F344 , Temperatura , Tempo , Transplante Isogênico , Difração de Raios X , Microtomografia por Raio-XRESUMO
The success rate of dental implants placed in female patients taking oral bisphosphonates, before the risks became known in 2003, were compared with a control group of females receiving implants and not taking bisphosphonates. The bisphosphonate group had an overall success rate of 86 percent versus a success rate of 95 percent in the control group. This suggests that the failure rate of implants placed in patients taking oral bisphosphonates may be higher unless suggested safeguards are taken.
