RESUMO
In experiments of curative vaccination, carried out with the use of an experimental model similar to the current practice of treatment with antirabies preparations, the advantages of using tissue-culture rabies vaccine with immunogenic potency equal to 1.3 international units (I. U.) were shown. In these experiments the vaccine was introduced into guinea pigs infected with fixed rabies virus, the course of vaccination consisting of 14 daily injections. No correlation between the induction of virus-neutralizing antibodies and the immunogenic potency of tissue-culture rabies vaccine was established: the use of the vaccine with immunogenic potency equal to 0,3 and 1,3 I.U. had no essential influence on the level of antibody formation in the animals.
Assuntos
Vacina Antirrábica/imunologia , Raiva/prevenção & controle , Animais , Anticorpos Antivirais/sangue , Avaliação Pré-Clínica de Medicamentos , Cobaias , Camundongos , Testes de Neutralização , Raiva/imunologia , Vacina Antirrábica/administração & dosagem , Vírus da Raiva/imunologia , Vírus da Raiva/patogenicidade , Fatores de TempoRESUMO
Cells from anti-HIV-positive persons were used in experiments for virus isolation. The RT-activity, viral antigen, nucleic acids, electron microscopic morphology, and infectivity were studied. The data presented allow a conclusion that the virus was isolated. These data confirmed the previous diagnosis of HIV infection.
Assuntos
Antígenos HIV/sangue , Soropositividade para HIV/imunologia , HIV-1/imunologia , Linfócitos/imunologia , Northern Blotting , Southern Blotting , Linhagem Celular , Células Cultivadas/imunologia , Células Cultivadas/microbiologia , DNA Viral/genética , Soropositividade para HIV/microbiologia , HIV-1/genética , HIV-1/ultraestrutura , Humanos , Linfócitos/microbiologia , Microscopia Eletrônica , Hibridização de Ácido Nucleico , RNA Viral/genética , DNA Polimerase Dirigida por RNA/análise , Vírion/ultraestruturaRESUMO
In this work materials on the development of an experimental model for the study of rabies vaccines are presented. The comparative study of different immunization schedules for vaccines with different protective potency has been carried out. Guinea pigs infected with street rabies virus, strain k, were used as an experimental model. As shown in this investigation the optimum method of infecting the animals with strain k was intramuscular injection causing 50% mortality among the animals, the incubation period lasting 10-24 days. Only those tissue-culture rabies vaccines which had activity equal to 1.0-1.3 I. U. and, when injected into the animals, ensured survival rate ranging from 57% to 76%, depending on the immunization schedule, were shown to possess protective potency. It should be pointed out that survival rate among the animals receiving the preparation according to the reduced schedules recommended by WHO was higher than among those immunized daily for 14 days. In all groups immune response was observed. Still in the animals receiving the preparation according to the reduced schedules a higher level of virus-neutralizing antibodies was registered. Thus, an experimental model capable of being used for the evaluation of the quality of existing and newly developed antirabies preparations was obtained. Besides, we believe it to be expedient to carry out the field trial of rabies vaccines with activity equal to 1.0-1.3 I. U., using the reduced immunization schedules.